Stage-dependent modulation of Sertoli cell steroid production in dogfish (Scyliorhinus canicula)

Sourdaine, Pascal; Garnier, Danielle Hélène

doi:10.1530/jrf.0.0970133

Cited by 27 publications

(16 citation statements)

References 20 publications

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“…After the development of a transillumination technique allowing the collection of freshly isolated spermatocytes of S. canicula (Sourdaine and Garnier, 1993; M. LOIR E T AL. Sourdaine and JBgou, 19891, it was possible to demonstrate that total protein secretion was highest from the start of spermiogenesis.…”

Section: Sertoli Cell-germ Cell Interactionsmentioning

confidence: 99%

Cell‐cell interactions in the testis of teleosts and elasmobranchs

Loir¹,

Sourdaine²,

Mendis‐Handagama³

et al. 1995

Microscopy Res & Technique

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In this paper we present the state of knowledge on cell-cell interactions in the testis of two groups of anamniote vertebrates--teleosts and elasmobranchs--which include most fish. In these fish, the structural organization of the testis differs fundamentally from that which characterizes amniotes in which the germinal tissue is located in tubules open at both ends and consists of a permanent population of Sertoli cells associated with successive stages of germ cell development. In fish, the spermatogenic unit of testis is the spermatocyst, which corresponds to one germ cell or to a clone of isogenetic germ cells, enclosed by one or several Sertoli cells, which form the wall of the cyst. In fish testis, the Sertoli cells do not represent a permanent population of cells. Although both are of the cystic type, the teleost and elasmobranch testes are differently organized. In elasmobranchs, primary spermatogonia and Sertoli cells lie initially free within the interstitial tissue, before becoming sequestered by a basement membrane; the testis is then composed of a mass of spermatocysts which contain many Sertoli cells, each being associated with a clone of germ cells. In contrast, in teleosts, the cysts are confined to large elongated structures limited by a basement membrane. These structures are either lobules originating under the albuginea or tubules which, in contrast to those of mammals, are anastomosed. In the lobules, the spermatocysts start to develop at the blind end of the lobules and migrate towards the efferent system, whereas in the tubules, the spermatocysts are located against the basement membrane, all along the tubules and do not migrate. In elasmobranchs, unlike teleosts, Leydig cells are either absent from the interstitial tissue or rare and undifferentiated and their role in steroid production is at best marginal. While many studies have focused on topographical and functional interactions between the diverse cell types present in mammalian testis, only a few studies have brought particular attention to these aspects in fish. In fish, like in mammals, testicular cell-cell interactions are based on structural elements and chemical factors. Occasionally, various adhering junctions have been observed, essentially in teleosts, between Sertoli cells, between Sertoli cells and germ cells, between germ cells themselves, and interstitial cells. Furthermore, in some teleost species, using horseradish peroxidase or lanthanum salts, the presence of tight junctions between Sertoli cells has been correlated to the occurrence of a Sertoli barrier. In these species, the barrier develops after meiosis so that only haploid germ cells are shielded from the vascular system. In fish, recent development of techniques which enable the preparation and in vitro culture of enriched populations of testicular cells and of spermatocysts, has allowed investigations on functional aspects of cell-cell interactions. In particular, data have been obtained, in the trout, on the control of spermatogonia proliferation by Sertoli cel...

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Section: Sertoli Cell-germ Cell Interactionsmentioning

confidence: 99%

Cell‐cell interactions in the testis of teleosts and elasmobranchs

Loir¹,

Sourdaine²,

Mendis‐Handagama³

et al. 1995

Microscopy Res & Technique

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show abstract

“…With regard to our previous results showing an increase of the testosterone content in testicular zones and in isolated spermatocysts containing late spermatids (Sourdaine et al 1990;Sourdaine and Garnier 1992), it may be possible that this steroid play a role in the synthesis of some polypeptides in cysts with late spermatids as observed in mammals. In fact, Sharpe et al (1992) have recently shown that the synthesis or the secretion of several proteins by rat seminiferous tubules (stages VI-VII) was regulated by testosterone.…”

Section: Id-sds-pa Gementioning

confidence: 74%

“…Thus, three (Callard et al 1985) or four (Sourdaine et al 1990) distinct zones, corresponding to the spermatocysts with spermatogonia (zone A: stages I-VI of Mellinger 1965), with spermatocytes (zone B, stages VII-X), with early spermatids (zone C, stages XI-XII) and with late spermatids (zone D, stages XIII-XVIII), can be microdissected. The testicular tissues can then be directly used for measuring biochemical parameters (Callard et al 1985;Sourdaine et al 1990) or dissociated to obtain isolated spermatocysts (Sourdaine and Jgou 1989;Dubois and Callard 1989;Sourdaine and Garnier 1992).…”

Section: Introductionmentioning

confidence: 99%

Cell-cell interactions in the testis of the dogfish: stage-related changes in protein synthesis

Sourdaine

Jégou

1993

Fish Physiol Biochem

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Previous studies have shown that the testis of Selachians is a very suited model to study stage-dependent changes in Sertoli cells during spermatogenesis (Dubois and Callard 1989; Sourdaine et al. 1990). In the dogfish testis (here: Scyliorhinus canicula), germ cells, at an identical stage of spermatogenesis, are associated with Sertoli cells to form spermatocysts, which are arranged in zones corresponding to the different stages of spermatogenesis. Using previously described methods for the isolation and culture of spermatocysts from four spermatogenic stages (spermatogonia, spermatocytes, early spermatids and late spermatids; Sourdaine and Jégou 1989; Sourdaine and Garnier 1992) and electrophoresis techniques (1D and 2D-SDS-PAGE) we have investigated the [(35)S] methionine incorporation into proteins in the dogfish testis. Our results indicate that protein synthesis reaches a maximum in spermatocysts with spermatocytes. Marked stage-related changes of protein synthesis and secretion were also observed on the autoradiograms of 1D and 2D-SDS-PAGE. Further investigations of the paracrine control of germ cells on Sertoli cell protein synthesis requires the identification of specific Sertoli cell proteins in the dogfish.

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“…Immature Sertoli cells do have a limited capacity to metabolize cholesterol and progesterone into androgens, as demonstrated in rats [Hall et al, 1969;Tcholakian and Steinberger, 1978] and in dogfish Scyliorhinus canicula [Sourdaine and Garnier, 1993]. In the immature spotted ray Torpedo marmorata , Sertoli cells already displayed the typical organization of a steroidogenic cell when Leydig cells were still fibroblastic [Prisco et al, 2002].…”

Section: Discussionmentioning

confidence: 99%

Early Expression of the Androgen Receptor in the Sertoli Cells of a Marsupial Coincides with Downregulation of Anti-Müllerian Hormone at the Time of Urogenital Virilization

Racine

Pask

Wijayanti

et al. 2009

Sex Dev

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Anti-Müllerian hormone (AMH), responsible for the regression of Müllerian ducts, is strongly expressed by eutherian fetal and postnatal Sertoli cells. Both AMH and testosterone levels are high during the period of fetal reproductive tract virilization which occurs largely in utero in eutherian mammals. Taking advantage of the fact that differentiation of the urogenital tract occurs after birth in marsupials, we studied the ontogeny and regulation of AMH in the tammar wallaby testis and related it to the expression of the androgen receptor in Sertoli cells. Testicular AMH expression was high between days 10–30 post partum, then fell to basal levels by day 60 and remained low until day 90, the oldest age examined. AMH expression was repressed by treatment of male pouch young with the potent androgen androstanediol. Thus, in the tammar, AMH expression decreases in response to androgen at the time of initial urogenital masculinization, in contrast to the situation in humans in which AMH is repressed by testosterone only at the time of puberty. The difference might be explained by the timing of androgen receptor expression which appears in tammar Sertoli cells at around day 40 of pouch life but only at a later developmental stage in eutherians.

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Stage-dependent modulation of Sertoli cell steroid production in dogfish (Scyliorhinus canicula)

Abstract: Seminiferous lobules of dogfish (Scyliorhinus canicula)

Cited by 27 publications

References 20 publications

Cell‐cell interactions in the testis of teleosts and elasmobranchs

Cell‐cell interactions in the testis of teleosts and elasmobranchs

Cell-cell interactions in the testis of the dogfish: stage-related changes in protein synthesis

Early Expression of the Androgen Receptor in the Sertoli Cells of a Marsupial Coincides with Downregulation of Anti-Müllerian Hormone at the Time of Urogenital Virilization

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