Stage‐dependent teratogenic and lethal effects exerted by ultraviolet B radiation on <i>Rhinella (Bufo</i>) arenarum embryos

Castañaga, Luis A.; Asorey, Cynthia M.; Sandoval, María Teresa; Pérez-Coll, Cristina S.; Argibay, Teresa I.; Herkovits, Jorge

doi:10.1897/08-144.1

Cited by 14 publications

(19 citation statements)

References 35 publications

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“…By considering ontogenic features such as stage-dependent biochemical or physiological features as well as stage-dependent susceptibility to noxious agents as biomarkers of the coevolution between the ancestors of living forms and environmental signatures during Earth's history [39], a new synthesis of the evolutionary process has been provided [13,39]. Does the stagedependent susceptibility to Ni reported in the present study reflect environmental changes during the evolutionary process?…”

Section: Discussionmentioning

confidence: 88%

“…The unexpected rate of decline in the amphibian population as well as the large number of teratogenic effects recorded worldwide [9,10] has led us to assess the potential toxicity of a large number of physical and chemical agents on amphibian species. Two standardized test methods, FETAX [11], used worldwide, and AMPHITOX (amphibian embryo-larval toxicity test) [12], a more recent test, have shown comparable adverse effects on amphibians for physical and chemical pollutants [13][14][15] as well as environmental samples [16].…”

Section: Introductionmentioning

confidence: 99%

“…In this context, understanding the stage-dependent susceptibility to noxious agents is of interest from an environmental perspective. Several contributions point out a remarkable stage-dependent susceptibility to chemicals [14,15,17,18] and physical agents [13]. By expanding the stage-dependent susceptibility studies to larval and metamorphic stages [19], a complete profile of susceptibility during the ontogenic period to each environmental agent could be reported.…”

Section: Introductionmentioning

confidence: 99%

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Nickel toxicity in embryos and larvae of the South American toad: Effects on cell differentiation, morphogenesis, and oxygen consumption

Sztrum

D'Eramo

Herkovits

2011

Enviro Toxic and Chemistry

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Nickel, a widely distributed heavy metal in the biosphere, produces systemic, carcinogenic, and teratogenic effects. The objectives of the present study are to report the acute, short-term chronic, and chronic toxicity of Ni in Rhinella arenarum embryos as well as the stage-dependent susceptibility to this heavy metal, including oxygen consumption, teratogenesis, and adverse effects on cell differentiation processes. The stages evaluated were blastula (S.7), gastrula (S.11), tail bud (S.17), fin circulation (S.22), and complete operculum (S.25), in this last case by means of toxicity profile curves. Nickel increases its adverse effects gradually, with a maximum value after 96 h. The 50% lethal concentrations (LC50s) for 96, 168, and 240 h at S.25 were 1.14, 0.60, and 0.48 mg Ni²(+) /L, respectively; S.11 and S.22 were the least and most susceptible to Ni with, LC50s 96 h of 6.12 and 0.19 mg Ni²(+) /L, respectively. A reduction of approximately 25% in oxygen consumption anticipates lethal effects from S.17 onward. The main teratogenic effects were retarded growth and development, extremely severe axis incurvations, persistent yolk plug, asymmetry, microcephaly and mouth and gill agenesia, and limited neuromuscular activity. Ciliated cells were not functional. The possibility of associating the remarkable stage-dependent susceptibility to Ni with environmental changes during the evolutionary process is also considered.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nickel toxicity in embryos and larvae of the South American toad: Effects on cell differentiation, morphogenesis, and oxygen consumption

Sztrum

D'Eramo

Herkovits

2011

Enviro Toxic and Chemistry

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“…Spinal curvature Ambystoma macrodactylum Ambient sunlight [191] Anaxyrus boreas Artificial lamps [194,195] Hypsiboas pulchellus Artificial lamps [69] Limnodynastes peronii Artificial lamps [65] Lithobates clamitans Artificial lamps [93] Lithobates pipiens Artificial lamps [58] Lithobates sylvaticus Ambient sunlight [188] Artificial lamps [93] Pleurodema bufoninum Ambient sunlight [92] Artificial lamps [92] Pseudacris regilla Artificial lamps [102] Rana temporaria Artificial lamps [63] Rhinella arenarum Artificial lamps [187] Xenopus laevis Artificial lamps [196] Edema Ambystoma macrodactylum Ambient sunlight [191] Lithobates pipiens Artificial lamps [58] Lithobates sylvaticus Ambient sunlight [188] Pleurodema bufoninum Ambient sunlight [92] Artificial lamps [92] Pseudacris regilla Artificial lamps [102] Rana cascadae Artificial lamps [102] Rhinella arenarum Artificial lamps [187] Underdeveloped gills Rhinella arenarum Artificial lamps [187] Eye abnormalities Anaxyrus boreas Artificial lamps [194,195] Lithobates pipiens Ambient sunlight [185] Rana aurora Ambient sunlight plus artificial lamps [198] Rana cascadae Artificial lamps [199] Pseudacris regilla Ambient sunlight plus artificial lamps [198] several amphibian species in North America, Europe, Australia and South America. However, UVR sensitivity varies among life stages, populations and species for many response variables [11].…”

Section: Effects Of Uvr On Amphibiansmentioning

confidence: 99%

Drivers of amphibian declines: effects of ultraviolet radiation and interactions with other environmental factors

Alton

Franklin

2017

Clim Chang Responses

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As a consequence of anthropogenic environmental change, the world is facing a possible sixth mass extinction event. The severity of this biodiversity crisis is exemplified by the rapid collapse of hundreds of amphibian populations around the world. Amphibian declines are associated with a range of factors including habitat loss/ modification, human utilisation, exotic/invasive species, environmental acidification and contamination, infectious disease, climate change, and increased ultraviolet-B radiation (UVBR) due to stratospheric ozone depletion. However, it is recognised that these factors rarely act in isolation and that amphibian declines are likely to be the result of complex interactions between multiple anthropogenic and natural factors. Here we present a synthesis of the effects of ultraviolet radiation (UVR) in isolation and in combination with a range of naturally occurring abiotic (temperature, aquatic pH, and aquatic hypoxia) and biotic (infectious disease, conspecific density, and predation) factors on amphibians. We highlight that examining the effects of UVR in the absence of other ecologically relevant environmental factors can greatly oversimplify and underestimate the effects of UVR on amphibians. We propose that the pathways that give rise to interactive effects between multiple environmental factors are likely to be mediated by the behavioural and physiological responses of amphibians to each of the factors in isolation. A sound understanding of these pathways can therefore be gained from the continued use of multi-factorial experimental studies in both the laboratory and the field. Such an understanding will provide the foundation for a strong theoretical framework that will allow researchers to predict the combinations of abiotic and biotic conditions that are likely to influence the persistence of amphibian populations under future environmental change.

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“…The decline of amphibian populations and the large number of malformations found in many geographic regions has caused increasing concern [4]. Some studies indicate that this could be related to their high susceptibility to contaminants, particularly during early life stages [5], in that this susceptibility has been reported with diverse physicochemical agents such as metals [6,7], pesticides [8,9], industrial [10] and pharmaceutical [11] chemicals, and ultraviolet B radiation [12,13]. In addition, the risk for adverse effects might be enhanced by the preference to breed in shallow, lentic, or ephemeral water bodies in which pollutants might be concentrated.…”

Section: Introductionmentioning

confidence: 99%

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

Aronzon

Sandoval

Herkovits

et al. 2011

Enviro Toxic and Chemistry

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Copper toxicity in different embryonic and larval stages of the common South American toad Rhinella arenarum was evaluated by means of continuous and 24-h pulse treatments in 12 different developmental stages. Lethal concentrations (LC) of 10, 50, and 90% of continuous treatment with Cu from early blastula (S.4), complete operculum (S.25), and hind limb bud (S.28) stages were plotted from 24 to 168 h, resulting from S.4 in a 24-h LC50 of 137 µg Cu(2+) /L and a 168-h LC50 of 19.5 µg Cu(2+) /L. This result was in agreement with pulse treatments that showed a high resistance to Cu at blastula and gastrula stages, whereas the organogenic period, between muscular response (S.18) and open mouth (S.21), was very susceptible to this metal. Continuous treatments from S.25 showed no significant differences along exposure time (168-h LC50 = 51 µg Cu(2+) /L), but in the case of S.28 toxicity increased slightly from a 24-h LC50 of 138.6 µg Cu(2+) /L to a 168-h LC50 of 104 µg Cu(2+) /L, pointing out that, although the larval period was significantly more resistant to Cu, there was also a remarkable stage-dependent susceptibility to this metal. Copper teratogenic potential was approximately two, and main adverse effects were reduced body size, axial flexure, microcephaly, acephaly, mouth malformations, agenesis of or underdeveloped gills, agenesis of or underdeveloped tail, and hydropsy. The results are discussed considering Cu toxicity mechanisms, an evolutionary perspective, and environmental protection.

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Stage‐dependent teratogenic and lethal effects exerted by ultraviolet B radiation on Rhinella (Bufo) arenarum embryos

Cited by 14 publications

References 35 publications

Nickel toxicity in embryos and larvae of the South American toad: Effects on cell differentiation, morphogenesis, and oxygen consumption

Nickel toxicity in embryos and larvae of the South American toad: Effects on cell differentiation, morphogenesis, and oxygen consumption

Drivers of amphibian declines: effects of ultraviolet radiation and interactions with other environmental factors

Stage‐dependent susceptibility to copper in Rhinella arenarum embryos and larvae

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