Stage-specific activation of the E74B promoter by low ecdysone concentrations in the wing disc of Bombyx mori

Wang, Huabing; Iwanaga, Masako; Kawasaki, Hideki

doi:10.1016/j.gene.2013.12.002

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…During larval development, E74B is induced by a low concentration of 20E and triggers transcription preferentially over E74A. [33][34][35] For instance, in an experiment the D. melanogaster E74A mutant died in the pre-pupal or adult stage, while the E74B mutant had a defect in pupal formation and died in the pre-pupal stage. 36 This suggests that the two isoforms of E74 performed different functions during larval-larval development and larval-adult metamorphosis.…”

Section: Discussionmentioning

confidence: 99%

“…The isoform‐specific regions encode different amino acid residues in the N‐terminal 25–27 so they may play different roles in the growth and development of insects. During larval development, E74B is induced by a low concentration of 20E and triggers transcription preferentially over E74A 33–35 . For instance, in an experiment the D. melanogaster E74A mutant died in the pre‐pupal or adult stage, while the E74B mutant had a defect in pupal formation and died in the pre‐pupal stage 36 .…”

Section: Discussionmentioning

confidence: 99%

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Effect of silencing the E74B gene on the development and metamorphosis of Helicoverpa armigera

Xu,

Li,

Zhang

et al. 2023

Pest Management Science

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BACKGROUNDThe growth and development transition of insects are mainly mediated by ecdysone. As one of the ecdysone‐induced transcription factors, E74 is involved in many physiological processes of insect growth and development. However, E74 and its function in Helicoverpa armigera remains unclear.RESULTSIn this study, E74B, a subtype of the E74, was identified for the first time in H. armigera. Bioinformatics analysis showed that H. armigera E74B shared the highest homology with E74B in Bombyx mori, which belongs to the E26 transformation‐specific (ETS) superfamily. The expression profile showed that the transcription level of HaE74B increased in the late stages of fourth to sixth instars compared with the early stages; it was also high in the pupa and midgut. Moreover, we investigated the function of HaE74B through RNA interference and 20E rescue experiments. The results showed silencing of E74B affected the molting and growth of larvae, resulting in the death of more than 60% of larvae. In addition, it also seriously affected the metamorphosis of H. armigera, which reduced the pupae rate, the eclosion rate of the pupae, and fecundity. Application of 20E partially restored the defects in the molting, development and pupae rate of H. armigera.CONCLUSIONTaken together, these results demonstrated that HaE74B plays a critical role in the growth, development, and metamorphosis of H. armigera, which serves as a molecular target and sets out a theoretical foundation for RNAi‐mediated control of this key pest. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of silencing the E74B gene on the development and metamorphosis of Helicoverpa armigera

Xu,

Li,

Zhang

et al. 2023

Pest Management Science

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Insect cuticular protein; gene expression, genomic structure, transcriptional regulation, speculated cuticular structure, clarified through the genomic analysis of Bombyx mori

Kawasaki

2024

Arch Insect Biochem Physiol

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JH and ecdysone signaling regulate insect metamorphosis through the master transcription factors, Krüppel homolog 1 (kr‐h1), Broad‐Complex (BR‐C), and E93. Ecdysone signaling activates successively expressed ecdysone responsive transcription factors (ERTFs), and the interaction between ERTFs determines the expression profiles of ERTFs themselves. Through the construction of expressed sequence tag (EST) database of Bombyx mori from many tissues, the existence of a large number of cuticular protein (CP) genes was identified in wing disc cDNA library of the 3 days after the start of wandering (W3). From the genomic analysis, 12 types of CP clusters of CP genes were identified. DNA sequences of CP genes revealed the duplication of CP genes, which suggests to reflect the insect evolution. These CP genes responded to ecdysone and ecdysone pulse; therefore, CP genes were applied for the analysis of transcriptional regulation by ERTF. The binding sites of ERTF have been reported to exist upstream of CP genes in several insects, and the activation of CP genes occurred by the binding of ERTFs. Through the analysis, the following were speculated; the successive appearance of ERTFs and the activation of target genes resulted in the successively produced CPs and cuticular layer. The sequence of the ERTF and CP gene expression was the same at larval to pupal and pupal to adult transformation. The involvement of several ERTFs in one CP gene expression was also clarified; BmorCPG12 belongs to group showing expression peak at W3 and was regulated by two ERTFs; BHR3 and ßFTZ‐F1, BmorCPH2 belongs to group showing expression peak at P0 and was regulated by two ERTFs; ßFTZ‐F1 and E74A. The involvement of BHR39 as a negative regulator of CP gene expression was found. Larval, pupal, and adult cuticular layers were supposed to be constructed by the combination of different and similar types of CPs, through the expressed timing of CP genes.

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Bombyx mori transcription factor, E74A, beneficially affects BmNPV infection through direct interaction

Wang

Cheng

et al. 2022

Pest Management Science

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BACKGROUND: Nucleopolyhedrovirus (NPV), one of the baculoviruses, is a promising biopesticide for pest control. Lepidopteran account for 70% of pests, therefore investigation on highly conserved genes associated with viral infections in the lepidopteran model, the silkworm, will serve as a valuable reference for improving the effectiveness of pest management. BmE74A is a member of the erythroblast transformation-specific (ETS) family of transcription factors in Bombyx mori, which we previously found to be highly conserved and closely associated with BmNPV. This study aimed to elucidate the role of BmE74A in viral infection.RESULTS: A significantly high expression of BmE74A in eggs indicated its important role in embryonic development, as did relatively high expressions in the hemolymph and midgut. Significant differences in BmE74A expression in different resistant strains after BmNPV infection suggested its involvement as a response to viral infection. Moreover, RNA interference (RNAi) and overexpression experiments confirmed the important role of BmE74A in promoting viral infection. BmNPV infection was significantly suppressed and enhanced by BmE74A knockdown and overexpression, respectively. Besides, BmE74A was found to regulate the expression of BmMdm2 and Bmp53. Furthermore, the binding of ETS, the functional domain of BmE74A, to occlusion-derived virus proteins was confirmed by far-western blotting, and four viral proteins that may interact with ETS proteins were identified by mass spectrometry. Similarly, a homolog of BmE74A in Spodoptera litura was also found to be involved in larval susceptibility to BmNPV. CONCLUSION: BmE74A promotes BmNPV proliferation by directly interacting with the virus, which may be related to the suppression of the p53 pathway.

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Stage-specific activation of the E74B promoter by low ecdysone concentrations in the wing disc of Bombyx mori

Cited by 5 publications

References 35 publications

Effect of silencing the E74B gene on the development and metamorphosis of Helicoverpa armigera

Effect of silencing the E74B gene on the development and metamorphosis of Helicoverpa armigera

Insect cuticular protein; gene expression, genomic structure, transcriptional regulation, speculated cuticular structure, clarified through the genomic analysis of Bombyx mori

Bombyx mori transcription factor, E74A, beneficially affects BmNPV infection through direct interaction

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