Stage‐specific changes in the levels of granulocyte‐macrophage colony‐stimulating factor and its receptor in the biological fluid and organ of mouse fetuses

Matsumoto, Akihiro; Hatta, Toshihisa; Ono, Atsuki; Hashimoto, Ryuju; Otani, Hiroki

doi:10.1111/j.1741-4520.2011.00337.x

Cited by 6 publications

(3 citation statements)

References 27 publications

(57 reference statements)

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“…FGF-basic, has been reported to stimulate the growth of cultured amniotic fluid cells, without harmful effects on chromosomes [ 22 ]. GM-CSF is a hematopoietic cytokine provided with neurotrophic and neuroprotective functions for which specific pregnancy-stage and organ modulation have been reported in mice foetuses [ 23 ]. Finally, G-CSF has been reported to improve pregnancy rate, reducing recurrent miscarriage in assisted reproduction [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of vaginal lactoferrin administration on amniotic fluid cytokines and its role against inflammatory complications of pregnancy

et al. 2017

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BackgroundAn altered amniotic cytokine profile has been reported in inflammatory pregnancy complications with a leading role for IL-6, a marker of the foetal systemic inflammatory response. Up to this date there is no exhaustive information neither on the foetal cytokine balance nor on the best method for its modulation. We aimed to evaluate the influence of vaginal lactoferrin administration on amniotic fluid concentration of 47 cytokines, chemokines and growth factors.MethodsSixty women undergoing genetic amniocentesis were enrolled in an open-label clinical trial. 300 mg of vaginal lactoferrin (Florence, Italy) were randomly administered to obtain 3 groups: A, 20 untreated patients; B and C (20 patients in each) respectively treated 4 and 12 h before amniocentesis. Cytokines, chemokines and growth factors concentrations were quantified by a magnetic bead Luminex multiplex immunoassays panel technology. Data analysis was performed with the software Stata (v. 13.1) and GraphPad Prism (v. 5). Group comparisons were performed using Kruskal–Wallis followed by Mann–Whitney U tests, with Bonferroni correction for multiple comparisons. A p < 0.05 was considered significant.ResultsAmong the 47 tested mediators, 24 (51.06%) were influenced by lactoferrin. 11 (23.4%), showed a highly significant difference (p <0.001); among these IL-9, IL-15, IFN-γ, IP-10, TNF-α, IL-1α and MCP-3 underwent a down-regulation, while IL-17 and FGF-basic, G-CSF, GM-CSF an up-regulation. Difference between group C and both B and A was small for IL-15, IP-10, IL-1α, MCP-3, while it was negligible for IL-9, IFN-γ and TNF-α. IL-17 and the 3 growth factors were strongly enhanced in B and C groups. IL-17, FGF-basic and GM-CSF showed increasing concentrations in both B and C groups, while G-CSF resulted up-regulated only in group C. Significance was intermediate (p < 0.01) for the down regulated IL-2RA, IL-12p40 and IFNα2 (6.38%) while it was small for 10 mediators (21.27%) 7 of which (IL-2, IL-4, eotaxin, PDGF-BB, RANTES, IL-18 and MIF) down-regulated and 3 (MCP-1, IL-3, and SDF-1α) up-regulated.ConclusionLactoferrin down-regulates 17 pro-inflammatory amniotic mediators while up-regulating 7 anti-inflammatory amniotic mediators, 5 of which definitively belonging to an anti-inflammatory profile. These findings open to clinical investigation on its use against inflammatory complications of pregnancy.

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Section: Discussionmentioning

confidence: 99%

Influence of vaginal lactoferrin administration on amniotic fluid cytokines and its role against inflammatory complications of pregnancy

et al. 2017

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“…Increased progesterone levels in amniotic fluid stimulate the development of tight intercellular junctions in amniotic epithelium, improving embryonic protection against maternal infections (Lagaye et al 2001;Kobayashi et al 2011). GM-CSF receptors are expressed in mouse embryos during mid-pregnancy (Matsumoto et al 2011) and the depletion of this cytokine delays embryonic growth, leading to metabolic disorders in postnatal stages (Gopichandran et al 2006;Robertson 2007).…”

Section: Discussionmentioning

confidence: 99%

Modulation of embryonic development due to mating with immunised males

Gerlinskaya

Maslennikova

Anisimova

et al. 2017

Reprod. Fertil. Dev.

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The modification of pre- and postnatal development conferred by immunogenic stimulation of mothers provides a population-level adaptation mechanism for non-genetic transfer of maternal experiences to progeny. However little is known about the transmission of paternal immune experiences to offspring. Here, we show that immune priming of males 3-9 days before mating affects the growth and humoral environment of developing embryos of outbred (ICR) and inbred (C57BL and BALB/c) mice. Antigenic stimulation of fathers caused a significant increase in embryonic bodyweight as measured on Day 16 of pregnancy and altered other gestation parameters, such as feto-placental ratio. Pregnant females mated with immunised males were also characterised by changes in humoral conditions as shown by measurements of blood and amniotic progesterone, testosterone and granulocyte-macrophage colony-stimulating factor (GM-CSF) cytokine concentrations. These results emphasise the role of paternal effects of immune priming on the in utero environment and fetal growth.

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“…It is well known that infection may cause an increase in maternal serum and amniotic fluid G-CSF values. In a literature search, the existence of G-CSF was described during clinically diagnosed intra-amniotic infection in amniotic fluid (14)(15)(16)(17), and its relationship to labor was reported as either increased serum levels of G-CSF or no difference between term-and preterm-delivered patients (4,5). In our study, any participant with any sign of infection was excluded in order to eliminate a bias in G-CSF levels.…”

Section: Discussionmentioning

confidence: 99%

Comparison of serum granulocyte colony-stimulating factor levels between preterm and term births

Kılıç

Uğur

Ünlü

et al. 2014

J Turkish German Gynecol Assoc

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Material and MethodsThis study was conducted between January 2011 and July 2011 in our perinatology clinic. Ethics approval was obtained from our institutional review board. Informed consent was given by all study participants. The study was designed as a prospective case-control study. PTB was defined as a delivery that occurs before the 37th week of gestation (9,10). Inclusion criteria in the study group were singleton pregnancies with a diagnosis of preterm labor leading to preterm birth at or before 37 weeks of gestation. Women who had preterm premature membrane rupture (P-PROM), clinical signs of intrauterine infection (uterine tenderness, fetal or maternal tachycardia, maternal fever, etc.), or bacterial infection in the cervical/vaginal culture were not included in the study or control groups. Also, women who had a history of maternal cardiac disease, cervical incompetence, cervical cerclage or conization, fetal anomaly, or vaginal bleeding were excluded from the study and control groups. A total of 600 singleton pregnant women were screened at 24-28 weeks of gestation and routinely followed up at the outpatient clinic until delivery. Demographic values of all pregnant women were recorded. Forty pregnant women delivered their infants spontaneously after preterm labor before 37 weeks of gestation. Also, 120 pregnant women were selected as a control group among these 560 women via a simple random sampling method, who delivered their infants after 37 weeks of gestation. All participants had 24-28-week plasma samples available for granulocyte colony-stimulating factor analysis. For both groups, maternal blood samples were collected at 24-28 weeks of gestation during a routine prenatal screening program in the outpatient clinic. Venous blood samples from patients were collected into sterile, silicone-coated tubes, centrifuged, and stored at -70°C. Maternal serum G-CSF levels were measured with the G-CSF Human Enzyme-Linked Immunosorbent Assay (ELISA) kit (Novex®; Invitrogen, Los Angeles, USA). The Statistical Package for the Social Sciences 14.0 (SPSS Inc.; Chicago, IL, USA) statistical package was used for the statistical analysis. Descriptive statistical methods (mean, standard deviation), as well as a comparison of quantitative data, MannWhitney U-test, chi-square test, and Student's t-test, were used for the determination of differences between the two groups. Statistically significant levels for the tests were set at a p value <0.05. Spearman's rank correlation coefficient rho was used for the correlation study. ResultsThe characteristics of 160 women and the comparison of the two groups are shown in Table 1. The difference of the mean age of the women between groups was not statistically significant (p=0.8). There were no significant differences in maternal serum G-CSF levels between groups (p=0.28). Smoking status was different between cases of PTB and fullterm pregnancies, but a statistically significant difference was not found (p=0.284). ROC curve analysis was performed for the prediction of...

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Stage‐specific changes in the levels of granulocyte‐macrophage colony‐stimulating factor and its receptor in the biological fluid and organ of mouse fetuses

Cited by 6 publications

References 27 publications

Influence of vaginal lactoferrin administration on amniotic fluid cytokines and its role against inflammatory complications of pregnancy

Influence of vaginal lactoferrin administration on amniotic fluid cytokines and its role against inflammatory complications of pregnancy

Modulation of embryonic development due to mating with immunised males

Comparison of serum granulocyte colony-stimulating factor levels between preterm and term births

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