Stage-specific expression of microRNAs during development

Watanabe, Takeru; Takeda, Atsushi; Mise, Kazuyuki; Okuno, Tetsuro; Suzuki, Tomoo; Minami, Naojiro; Imai, Hisanori

doi:10.1016/j.febslet.2004.11.089

Cited by 58 publications

(89 citation statements)

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“…The amount detected for all but one analyzed miRNA increased significantly within this period and several miRNAs were not detectable at 1.5 and 2 days even after 4 days exposure. This general pattern is very similar to that observed in Xenopus (Watanabe et al, 2005) and recently in zebrafish embryos (Chen et al, 2005). The exception was mir-302, which was very abundant at 1.5 and 2 days but was not detectable after 3 days.…”

Section: Characterization Of Chicken Mirnassupporting

confidence: 88%

FGF‐4 signaling is involved in mir‐206 expression in developing somites of chicken embryos

Sweetman

Rathjen

Jefferson

et al. 2006

Developmental Dynamics

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The microRNAs (miRNAs) are recently discovered short, noncoding RNAs, that regulate gene expression in metazoans. We have cloned short RNAs from chicken embryos and identified five new chicken miRNA genes. Genome analysis identified 17 new chicken miRNA genes based on sequence homology to previously characterized mouse miRNAs. Developmental Northern blots of chick embryos showed increased accumulation of most miRNAs analyzed from 1.5 days to 5 days except, the stem cell-specific mir-302, which was expressed at high levels at early stages and then declined. In situ analysis of mature miRNAs revealed the restricted expression of mir-124 in the central nervous system and of mir-206 in developing somites, in particular the developing myotome. In addition, we investigated how miR-206 expression is controlled during somite development using bead implants. These experiments demonstrate that fibroblast growth factor (FGF) -mediated signaling negatively regulates the initiation of mir-206 gene expression. This may be mediated through the effects of FGF on somite differentiation. These data provide the first demonstration that developmental signaling pathways affect miRNA expression. Thus far, miRNAs have not been studied extensively in chicken embryos, and our results show that this system can complement other model organisms to investigate the regulation of many other miRNAs. Developmental Dynamics 235:2185-2191, 2006.

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Section: Characterization Of Chicken Mirnassupporting

confidence: 88%

FGF‐4 signaling is involved in mir‐206 expression in developing somites of chicken embryos

Sweetman

Rathjen

Jefferson

et al. 2006

Developmental Dynamics

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“…15 This study, unveiled the presence of three type of miRNA expression profiled. In the first category, for example, miR-15 and miR-16 are expressed maternally in the oocyte, and maintained zygotically throughout embryonic developmental window.…”

Section: Xenopusmentioning

confidence: 99%

“…As in the case of miR-430, 7 miR-427 expression starts at the onset of zygotic transcription and is highly abundant in early stages of development. 14,15 A crucial step during vertebrate development corresponds to the establishment of organizing centers that induce and pattern the surrounding tissue. In amphibians, one of the earliest organizing center of the embryo, called "Spemann's organizer" is formed in the dorsal-equatorial part of the embryo.…”

Section: Xenopusmentioning

confidence: 99%

MicroRNAs in early vertebrate development

Rosa

Brivanlou

2009

Cell Cycle

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“…While previous work in Xenopus has identified a number of miRNAs through cloning and comparative genomic approaches, little is know about small RNAs population during Xenopus oogenesis. Microarrays, Northern blotting, and in situ hybridization have been used to determine miRNA expression during embryogenesis and adult frog (Watanabe et al 2005;Hikosaka et al 2007;Michalak and Malone 2008;Tang and Maxwell 2008;Walker and Harland 2008). However, recent advances in sequencing technology have allowed the more complete assessment of small RNA species in animals, plants, and fungi.…”

mentioning

confidence: 99%

“…The lin-4 and let-7 miRNAs were the first type of endogenous short regulatory RNAs to be identified in eukaryotes (Lee et al 1993;Reinhart et al 2000); since then many functional small RNAs have been identified in organisms as diverse as roundworms, flies, fish, frogs, mammals, flowering plants, mosses, anemones, sponges, and even viruses, using genetics, molecular cloning, and predictions from bioinformatics (Lagos-Quintana et al 2001;Lau et al 2001;Lee and Ambros 2001;Llave et al 2002;Reinhart et al 2002;Lim et al 2003;Pfeffer et al 2004;Arazi et al 2005;Axtell and Bartel 2005;Watanabe et al 2005;Grimson et al 2008). In cells, miRNAs are tightly bound by proteins of the Ago clade of the Argonaute superfamily of RNA-binding proteins (Cerutti et al 2000).…”

mentioning

confidence: 99%

Abundant and dynamically expressed miRNAs, piRNAs, and other small RNAs in the vertebrate Xenopus tropicalis

Armisen¹,

Gilchrist²,

Wilczynska³

et al. 2009

Genome Res.

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Small regulatory RNAs have recently emerged as key regulators of eukaryotic gene expression. Here we used highthroughput sequencing to determine small RNA populations in the germline and soma of the African clawed frog Xenopus tropicalis. We identified a number of miRNAs that were expressed in the female germline. miRNA expression profiling revealed that miR-202-5p is an oocyte-enriched miRNA. We identified two novel miRNAs that were expressed in the soma. In addition, we sequenced large numbers of Piwi-associated RNAs (piRNAs) and other endogenous small RNAs, likely representing endogenous siRNAs (endo-siRNAs). Of these, only piRNAs were restricted to the germline, suggesting that endo-siRNAs are an abundant class of small RNAs in the vertebrate soma. In the germline, both endogenous small RNAs and piRNAs mapped to many high copy number loci. Furthermore, endogenous small RNAs mapped to the same specific subsets of repetitive elements in both the soma and the germline, suggesting that these RNAs might act to silence repetitive elements in both compartments. Data presented here suggest a conserved role for miRNAs in the vertebrate germline. Furthermore, this study provides a basis for the functional analysis of small regulatory RNAs in an important vertebrate model system.

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Stage-specific expression of microRNAs during development

Cited by 58 publications

References 0 publications

FGF‐4 signaling is involved in mir‐206 expression in developing somites of chicken embryos

FGF‐4 signaling is involved in mir‐206 expression in developing somites of chicken embryos

MicroRNAs in early vertebrate development

Abundant and dynamically expressed miRNAs, piRNAs, and other small RNAs in the vertebrate Xenopus tropicalis

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