Stage-specific protein regulation during somatic embryo development of Carica papaya L. ‘Golden’

Botini, Nadia; Almeida, Felipe Astolpho; Cruz, Kaliane Zaira Camacho Maximiano; Reis, Robert L.; Vale, Ellen Moura; Garcia, Anice; Santa–Catarina, Claudete; Silveira, Vanildo

doi:10.1016/j.bbapap.2020.140561

Cited by 15 publications

(11 citation statements)

References 64 publications

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“…PAs are extremely important for plants and are related to abiotic and biotic stress tolerance (Zou et al, 2021). Also, the Pas vary between species and the developmental stages of SE (Minocha et al, 1999;Silveira et al, 2004;Kuznetsov and Shevyakova, 2007;de Oliveira et al, 2020;Botini et al, 2021). In our work, the amount of PAs did not change with different treatments assayed in P. radiata.…”

Section: Discussionsupporting

confidence: 43%

The Chemical Environment at Maturation Stage in Pinus spp. Somatic Embryogenesis: Implications in the Polyamine Profile of Somatic Embryos and Morphological Characteristics of the Developed Plantlets

Nascimento¹,

Polesi

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et al. 2021

Front. Plant Sci.

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Changes in the chemical environment at the maturation stage in Pinus spp. somatic embryogenesis will be a determinant factor in the conversion of somatic embryos to plantlets. Furthermore, the study of biochemical and morphological aspects of the somatic embryos could enable the improvement of somatic embryogenesis in Pinus spp. In the present work, the influence of different amino acid combinations, carbohydrate sources, and concentrations at the maturation stage of Pinus radiata D. Don and Pinus halepensis Mill. was analyzed. In P. radiata, the maturation medium supplemented with 175 mM of sucrose and an increase in the amino acid mixture (1,100 mgL–1 of L-glutamine, 1,050 mgL–1 of L-asparagine, 350 mgL–1 of L-arginine, and 35 mgL–1 of L-proline) promoted bigger embryos, with a larger stem diameter and an increase in the number of roots in the germinated somatic embryos, improving the acclimatization success of this species. In P. halepensis, the maturation medium supplemented with 175 mM of maltose improved the germination of somatic embryos. The increase in the amount of amino acids in the maturation medium increased the levels of putrescine in the germinated somatic embryos of P. halepensis. We detected significant differences in the amounts of polyamines between somatic plantlets of P. radiata and P. halepensis; putrescine was less abundant in both species. For the first time, in P. radiata and P. halepensis somatic embryogenesis, we detected the presence of cadaverine, and its concentration changed according to the species.

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Section: Discussionsupporting

confidence: 43%

The Chemical Environment at Maturation Stage in Pinus spp. Somatic Embryogenesis: Implications in the Polyamine Profile of Somatic Embryos and Morphological Characteristics of the Developed Plantlets

Nascimento¹,

Polesi

Back

et al. 2021

Front. Plant Sci.

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“…PGRs act synergistically at different stages of somatic embryos [24]. Auxin and cytokinin combinations work together to promote the initiation of somatic embryos [25]. Our study observed that embryogenic callus budding initiated within 22 days of inoculation and that seedlings formed at 45 days after culture.…”

Section: Discussionmentioning

confidence: 55%

Somatic Embryogenesis and Indirect In Vitro Plant Regeneration in Amorphophallus konjac K. Koch by One-Step Seedling Formation

Mohammadi

Qin

et al. 2021

Horticulturae

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Konjac (Amorphophallus konjac K. Koch) is a well-known tuberous vegetable belonging to the important medicinal family Araceae, and the plant grows from an underground tuber. Here, we used a “one-step seedling regeneration” tissue culture system to improve the plantlet regeneration efficiency of konjac using young leaves as an explant source. In the current study, we used several sterilization methods for tuber sterilization. Moreover, various plant growth regulator combinations were applied to achieve efficient somatic embryogenesis and plantlet regeneration. Our results showed that the optimal tuber sterilization was method C (75% alcohol for 15 s + 0.1% HgCl2 for 15 min + washing by double-sterilized water three times). Three types of embryogenic calli were induced on full-strength Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L of 6-benzylaminopurine (6-BA), 0.5 mg/L of naphthaleneacetic acid (NAA), 1.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D), and 30 g/L of sucrose. Of the three types of embryogenic calli, only type Ⅲ further regenerated plantlets, with a callus induction rate of 55.73% and a seedling induction rate of 92.73%. This suggests that the addition of the above hormones gives the optimal callus induction. The proliferation rate achieved was 38% on the MS basal medium containing 1.0 mg/L of 6-BA, 1.0 mg/L of indolebutyric acid (IBA), 0.2 mg/L of kinetin (KT), and 50 g/L of sucrose. The one-step seedling formation achieved in MS medium contained 2.0 mg/L of 6-BA, 0.5 mg/L of NAA, 0.1 mg/L of gibberellic acid (GA3), and 30 g/L of sucrose, and the number of regenerated shoots per explants was 6 ± 2. Therefore, we establish a one-step seedling regeneration system through indirect plant regeneration, which shortens the time for konjac in vitro regeneration, significantly increased the micropropagation efficiency, and decreased the cost of the konjac tissue culture.

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“…Proteomics analysis was performed using a nanoACQUITY ultraperformance liquid chromatograph (UPLC) coupled to a Q-TOF SYNAPT G2-Si instrument (Waters, Manchester, UK) with the parameters described by Botini et al (2021). Briefly, the runs consisted of five biological replicates; in each run, 1 μg of the digested proteins were loaded onto a nanoACQUITY UPLC M-Class Symmetry C18 5 μm trap column (180 μm × 20 mm) at 5 μL min -1 for 3 min and then onto a nanoACQUITY M-Class HSS T3 1.8 μm analytical reversed-phase column (75 μm × 150 mm) at 400 nL min -1 .…”

Section: Proteomics Analysismentioning

confidence: 99%

Integrative proteomics and phosphoproteomics reveals phosphorylation networks involved in the maintenance and expression of embryogenic competence in sugarcane callus

Xavier

Almeida

Pinto

et al. 2022

Journal of Plant Physiology

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Stage-specific protein regulation during somatic embryo development of Carica papaya L. ‘Golden’

Cited by 15 publications

References 64 publications

The Chemical Environment at Maturation Stage in Pinus spp. Somatic Embryogenesis: Implications in the Polyamine Profile of Somatic Embryos and Morphological Characteristics of the Developed Plantlets

The Chemical Environment at Maturation Stage in Pinus spp. Somatic Embryogenesis: Implications in the Polyamine Profile of Somatic Embryos and Morphological Characteristics of the Developed Plantlets

Somatic Embryogenesis and Indirect In Vitro Plant Regeneration in Amorphophallus konjac K. Koch by One-Step Seedling Formation

Integrative proteomics and phosphoproteomics reveals phosphorylation networks involved in the maintenance and expression of embryogenic competence in sugarcane callus

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