Stage-specific regulation of juvenile hormone biosynthesis by ecdysteroid in Bombyx mori

Kaneko, Yu; Kinjoh, Terunori; Kiuchi, Makoto; Hiruma, Kiyoshi

doi:10.1016/j.mce.2011.01.012

Cited by 17 publications

(15 citation statements)

References 34 publications

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“…JH biosynthesis is irreversibly stimulated in cultured Aedes aegypti CA following 20-hydroxyecdysone (20E) addition at a concentration of 10 −6 M 43 . Fourth instar Bombyx mori CC-CA complexes likewise increased JH biosynthesis when cultured with 20E in a narrow concentration range, and this JH synthesis profile was concomitant with the elevation and subsequent decline in the transcript abundance of two JH biosynthetic enzymes 44 . In both A .…”

Section: Discussionmentioning

confidence: 87%

Genetic tools to study juvenile hormone action in Drosophila

Baumann

Texada

Chen

et al. 2017

Sci Rep

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The insect juvenile hormone receptor is a basic helix-loop-helix (bHLH), Per-Arnt-Sim (PAS) domain protein, a novel type of hormone receptor. In higher flies like Drosophila, the ancestral receptor germ cell-expressed (gce) gene has duplicated to yield the paralog Methoprene-tolerant (Met). These paralogous receptors share redundant function during development but play unique roles in adults. Some aspects of JH function apparently require one receptor or the other. To provide a foundation for studying JH receptor function, we have recapitulated endogenous JH receptor expression with single cell resolution. Using Bacteria Artificial Chromosome (BAC) recombineering and a transgenic knock-in, we have generated a spatiotemporal expressional atlas of Met and gce throughout development. We demonstrate JH receptor expression in known JH target tissues, in which temporal expression corresponds with periods of hormone sensitivity. Larval expression largely supports the notion of functional redundancy. Furthermore, we provide the neuroanatomical distribution of JH receptors in both the larval and adult central nervous system, which will serve as a platform for future studies regarding JH action on insect behavior.

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Section: Discussionmentioning

confidence: 87%

Genetic tools to study juvenile hormone action in Drosophila

Baumann

Texada

Chen

et al. 2017

Sci Rep

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“…In all cases, the affinity of the metal cations for the diphosphate was more than 200 kcal/mol higher than for the aspartate residues. Based on these gas phase calculations, the deduced minimum equilibrium constant for formation of the diphosphate metal complex is at least 10 28 (Tables S5 and S6), in both cases, the affinities for Co 2+ or Mn 2+ are lower than the affinity for Mg 2+ in nice agreement with the measured lower K m (GDP) (higher affinity) (Mg 2+ = 1.18 μM, Co 2+ = 24.3 μM) and might be an explanation for the experimental differences in product specificity with different divalent metal ions. Note, one order of magnitude in K m represents 1.4 kcal/mol (ΔG) in interaction energies.…”

Section: Metal Cofactors Identified In P Cochleariae Larvae Have Difmentioning

confidence: 99%

Metal ions control product specificity of isoprenyl diphosphate synthases in the insect terpenoid pathway

Frick¹,

Nagel

Schmidt

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Isoprenyl diphosphate synthases (IDSs) produce the ubiquitous branched-chain diphosphates of different lengths that are precursors of all major classes of terpenes. Typically, individual shortchain IDSs (scIDSs) make the C 10 , C 15 , and C 20 isoprenyl diphosphates separately. Here, we report that the product length synthesized by a single scIDS shifts depending on the divalent metal cofactor present. This previously undescribed mechanism of carbon chainlength determination was discovered for a scIDS from juvenile horseradish leaf beetles, Phaedon cochleariae. The recombinant enzyme P. cochleariae isoprenyl diphosphate synthase 1 (PcIDS1) yields 96% C 10 -geranyl diphosphate (GDP) and only 4% C 15 -farnesyl diphosphate (FDP) in the presence of Co 2+ or Mn 2+ as a cofactor, whereas it yields only 18% C 10 GDP but 82% C 15 FDP in the presence of Mg 2+. In reaction with Co 2+ , PcIDS1 has a K m of 11.6 μM for dimethylallyl diphosphate as a cosubstrate and 24.3 μM for GDP. However, with Mg 2+ , PcIDS1 has a K m of 1.18 μM for GDP, suggesting that this substrate is favored by the enzyme under such conditions. RNAi targeting PcIDS1 revealed the participation of this enzyme in the de novo synthesis of defensive monoterpenoids in the beetle larvae. As an FDP synthase, PcIDS1 could be associated with the formation of sesquiterpenes, such as juvenile hormones. Detection of Co , or Mg 2+ in the beetle larvae suggests flux control into C 10 vs. C 15 isoprenoids could be accomplished by these ions in vivo. The dependence of product chain length of scIDSs on metal cofactor identity introduces an additional regulation for these branch point enzymes of terpene metabolism.cobalt | kinetic | prenyltransferase | secretions | silencing

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“…Aside from the naturally occurring agents regulating JH synthesis such as various neuropeptides (Stay and Tobe, 2007;Audsley et al, 2008;Yamanaka et al, 2008), 20E (Kaneko et al, 2011b), etc., there are possible three types of mode of actions by which synthetic compounds might act to block JH synthesis; allatocidal action, inhibition of JH biosynthetic enzyme activities, and inhibition of biosynthetic enzyme synthesis. The well-known anti-JH, precocene, inhibits JH synthesis by its allatocidal action (Schooneveld, 1979;Bowers and Soderlund, 1981).…”

Section: Discussionmentioning

confidence: 99%