Staining for viability testing, germination and maturation of <i>Sambucus nigra</i> L. pollen in vitro

Kosel, Janez; Vižintin, Liliana; Majer, Aljaz; Bohanec, B.

doi:10.1080/10520295.2018.1425912

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…Sucrose plays an extremely important role and is a significant nutrient for pollen germination (Fei & Nelson 2003, Liu et al 2013. It helps to protect membranes and preserve protein structure from desiccation, because pollen grains lose water rapidly after release (Kosel et al 2018). Huang et al (2011) reported that pollen germination of C. oleifera was best when sucrose concentration was 150 g•L -1 and began to decrease at 200 g•L -1 .…”

Section: Discussionmentioning

confidence: 99%

Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Zhao

Yuan

et al. 2021

An. Acad. Bras. Ciênc.

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Oil Tea (Camellia oleifera) is an important woody edible oil plant in China. Oil Tea suffers from low rate of fruit set during production, which is related to poor pollination and fertilization. Pollen vigor is directly related to pollination and fertilization. Using the interspecifi c hybrid Y3 (C. grijsii × C. oleifera) as plant material, we studied the effects of sucrose, H 3 BO 3 , MgSO 4 , and IAA on pollen germination using an orthogonal design to determine the best culture medium. Results indicated that pollen germination rates were signifi cantly affected by medium components and ranged from 29.13% to 56.84%. Pollen tube length was the longest in the T5 medium surpassing the control group by 489.36 μm. MgSO 4 turned out to be the most important germination medium component having great effect on the pollen germination rate. The optimal culture medium to promote pollen tube growth of Oil Tea Y3 was: 1% agar, 150 g•L-1 sucrose, 0.15 g•L-1 H 3 BO 3 , 0.07 g•L-1 MgSO 4 , and 0.01 g•L-1 IAA. The results of this paper may provide information for foliar application of Mg and IAA, which can improve pollen tube growth of Oil Tea in practice.

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Section: Discussionmentioning

confidence: 99%

Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Zhao

Yuan

et al. 2021

An. Acad. Bras. Ciênc.

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“…Microscopic examination of their florets discovered that both MOMT4 and MOMT9 transgenic lines developed aberrant white anthers that failed in producing viable mature pollen grains, as indicated by much less or no staining with iodine potassium iodide (IKI) (Figure 1f–n ). The IKI stain is widely used for monitoring starch; accumulation of starch in pollen grains is an indicator for pollen maturation (Kosel et al ., 2018 ). Consequently, the impaired (or the lack of) pollen grains caused sterility of the transgenic plants.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous suppression of lignin, tricin and wall‐bound phenolic biosynthesis via the expression of monolignol 4‐O‐methyltransferases in rice

Dwivedi,

Yamamoto,

Zhao

et al. 2023

Plant Biotechnology Journal

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SummaryGrass lignocelluloses feature complex compositions and structures. In addition to the presence of conventional lignin units from monolignols, acylated monolignols and flavonoid tricin also incorporate into lignin polymer; moreover, hydroxycinnamates, particularly ferulate, cross‐link arabinoxylan chains with each other and/or with lignin polymers. These structural complexities make grass lignocellulosics difficult to optimize for effective agro‐industrial applications. In the present study, we assess the applications of two engineered monolignol 4‐O‐methyltransferases (MOMTs) in modifying rice lignocellulosic properties. Two MOMTs confer regiospecific para‐methylation of monolignols but with different catalytic preferences. The expression of MOMTs in rice resulted in differential but drastic suppression of lignin deposition, showing more than 50% decrease in guaiacyl lignin and up to an 90% reduction in syringyl lignin in transgenic lines. Moreover, the levels of arabinoxylan‐bound ferulate were reduced by up to 50%, and the levels of tricin in lignin fraction were also substantially reduced. Concomitantly, up to 11 μmol/g of the methanol‐extractable 4‐O‐methylated ferulic acid and 5–7 μmol/g 4‐O‐methylated sinapic acid were accumulated in MOMT transgenic lines. Both MOMTs in vitro displayed discernible substrate promiscuity towards a range of phenolics in addition to the dominant substrate monolignols, which partially explains their broad effects on grass phenolic biosynthesis. The cell wall structural and compositional changes resulted in up to 30% increase in saccharification yield of the de‐starched rice straw biomass after diluted acid‐pretreatment. These results demonstrate an effective strategy to tailor complex grass cell walls to generate improved cellulosic feedstocks for the fermentable sugar‐based production of biofuel and bio‐chemicals.

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“…These methods are characterized by intuitiveness and rapidity, and depend mainly on cell integrity, enzyme activity, and nutritional components [35]. Pollen viability of different plants can be assessed with specific staining methods [42]. Currently, there are very limited comparisons of staining methods for pollen, especially in the assessment of P. ostii pollen.…”

Section: Discussionmentioning

confidence: 99%

Optimization of In Vitro Germination, Viability Tests and Storage of Paeonia ostii Pollen

Jiang

et al. 2023

Plants

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Paeonia ostii is an important woody oil crop mainly cross-pollinated. However, the low yield has become an important factor restricting the industrial development of P. ostii. Cross-pollination has become one of the important measures to increase the seed yield. Therefore, conservation of pollen with high vitality is crucial to ensure successful pollination of P. ostii. In this study, we found an effective methodological system to assess the viability, ability to germinate, and optimal storage conditions of P. ostii pollen grains. The optimal medium in vitro was 50 g/L sucrose, 100 mg/L boric acid, 50 g/L PEG6000, 100 mg/L potassium nitrate, 300 mg/L calcium nitrate, and 200 mg/L magnesium sulfate at pH 5.4. Optimal germination condition in vitro was achieved at 25 °C for 120 min, allowing easy observation of the germination percentage and length of the pollen tubes. In addition, the viability of pollen grains was assessed by comparing nine staining methods. Among them, MTT, TTC, benzidine-H2O2, and FDA were effective to distinguish between viable and non-viable pollen, and the results of the FDA staining method were similar to the pollen germination percentage in vitro. After evaluation of pollen storage, thawing and rehydration experiments showed that thawing at 4 °C for 30 min and rehydration at 25 °C for 30 min increased the germination percentage of pollen grains stored at low temperatures. The low-temperature storage experiments showed that 4 °C was suitable for short-term storage of P. ostii pollen grains, while −80 °C was suitable for long-term storage. This is the first report on the in vitro germination, viability tests, and storage of P. ostii pollen grains, which will provide useful information for P. ostii germplasm conservation and artificial pollination.

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Staining for viability testing, germination and maturation of Sambucus nigra L. pollen in vitro

Cited by 6 publications

References 23 publications

Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Simultaneous suppression of lignin, tricin and wall‐bound phenolic biosynthesis via the expression of monolignol 4‐O‐methyltransferases in rice

Optimization of In Vitro Germination, Viability Tests and Storage of Paeonia ostii Pollen

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