Staining internal limiting membrane with a mixture of BBG and sodium hyaluronate

Abstract

Visco-BBG can be a useful method to assist macular surgery and can overcome some of the disadvantages of conventional BBG solutions dissolved in BSS.

The Staining Pattern of Brilliant Blue G During Macular Hole Surgery: A Clinicopathologic Study

“…It is possible that differing concentrations and formulations of BBG could alter the staining patterns observed. 25 Importantly, this study has provided a direct clinicopathologic correlation between the staining patterns of the ILMspecific dye BBG and ILM vitreous surface tissue. Inner limiting membrane peeling has been shown to improve closure rate in MH surgery.…”

Clinical and histological evaluation of large macular hole surgery using the inverted internal limiting membrane flap technique

“…These cells were positive for GFAP, suggesting glial cell infiltration. Uemoto et al10 reported that BBG-positive ILM isolated from MH and epiretinal membrane revealed a membrane with few cell infiltration based on pathological analysis. Therefore, the BBG-negative case shown in this report may represent more glial cell infiltration within the ILM tissue than ILM stained with BBG.…”

Brilliant blue G assisted macular surgery: the effect of air infusion on contrast recognisability in internal limiting membrane peeling

“…While BBG selectively stains the ILM, staining intensity has been reported to be significantly inferior to that of ICG 9 14 15. A number of methods to promote intraoperative BBG sedimentation, such as dye cooling16 and a solution in 4% polyethylene glycol,4 and 5% glucose17 and visco-BBG18 and heavy-water BBG (BBG-D 2 O) have been suggested. However, the only objective measurement of BBG's staining characteristics with CIELAB scores was evaluated for BBG-D 2 O; objective chromaticity scores suggested that ILM staining is not significantly improved with the new formula 9…”