Staining methods applied to glycol methacrylate embedded tissue sections

Cerri, Paulo Sérgio; Sasso-Cerri, Estela

doi:10.1016/s0968-4328(03)00098-2

Cited by 98 publications

(74 citation statements)

References 18 publications

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“…However, it was reported that optimal morphological integrity and good results with H&E may be obtained also in the GMA-embedded tissues by using modified staining procedures [16]. details, like e.g.…”

Section: Resultsmentioning

confidence: 99%

“…For such structures, hard embedding materials such as glycol methacrylate (GMA; (2-hydroxyethyl)-methacrylate, [HEMA]) or methyl methacrylate (MMA) are required [15]. Although using GMA-based embedding medium for soft tissues is thought to provide a better morphological analysis as it prevents some technical artefacts observed in paraffin-embedded sections, it has been reported that staining may be poor in resin sections [16].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of three different techniques for histological tooth preparation

Kekli̇koğlu

Akinci²

2014

Folia Histochem Cytobiol.

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Abstract:The histological processing of teeth is highly complicated because of containing both mineralized hard tissues and soft tissues. Depending on the type of decalcification agents used in processing, mild-to-severe deterioration in the tissue structure and inadequacies on clear staining of details by the histological stain may be observed. This study aims to compare the histological staining differences in the preparations from decalcified and undecalcified tooth roots by three different embedding materials and techniques. Following extraction, human single-rooted teeth crowns were cut off and roots were placed in 10% buffered neutral formalin. After fixation, roots were divided into two groups. One part of samples was decalcified in formic acid solution and the other was remained undecalcified. Decalcified roots were embedded in paraffin and glycol methacrylate (GMA)-based resin and undecalcified roots were embedded in methyl methacrylate (MMA)-based resin. Sections from all groups were stained with hematoxylin and eosin. The groups were compared in terms of general staining, brightness, density, density of the base stain, general morphology of cells, nuclear/cytoplasmic contrast, distinguishability of pulp, odontoblast layer, predentin and dentin, preservation and traceability of dentinal tubule. In the preparations which were embedded into the MMA-based embedding material, an output lower than the paraffin group but higher than the GMA-embedded group was provided. As a result, the best histological detail was obtained from the decalcified, paraffin-embedded sections.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of three different techniques for histological tooth preparation

Kekli̇koğlu

Akinci²

2014

Folia Histochem Cytobiol.

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“…Eightmicroliter-thick sections were stained in toluidine blue solution (0.1% toluidine blue and 0.1 M sodium phosphate buffer, pH 5.0) for 1 min. After destaining in running water, sections were dried and mounted (Cerri and Sasso-Cerri, 2003). Images were taken on a Zeiss Stemi SV11 microscope with a Nikon COOLPIX 4500 digital camera.…”

Section: Histology and Microscopymentioning

confidence: 99%

Identification of Tyrosyl-DNA Phosphodiesterase as a Novel DNA Damage Repair Enzyme in Arabidopsis

et al. 2010

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Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is a key enzyme that hydrolyzes the phosphodiester bond between tyrosine of topoisomerase and 3#-phosphate of DNA and repairs topoisomerase-mediated DNA damage during chromosome metabolism. However, functional Tdp1 has only been described in yeast and human to date. In human, mutations of the Tdp1 gene are involved in the disease spinocerebellar ataxia with axonal neuropathy. In plants, we have identified the functional nuclear protein AtTDP, homolog to human Tdp1 from Arabidopsis (Arabidopsis thaliana). The recombinant AtTDP protein certainly hydrolyzes the 3#-phosphotyrosyl DNA substrates related to repairing in vivo topoisomerase I-DNA-induced damage. The loss-of-function AtTDP mutation displays developmental defects and dwarf phenotype in Arabidopsis. This phenotype is substantially caused by decreased cell numbers without any change of individual cell sizes. The tdp plants exhibit hypersensitivities to camptothecin, a potent topoisomerase I inhibitor, and show rigorous cell death in cotyledons and rosette leaves, suggesting the failure of DNA damage repair in tdp mutants. These results indicate that AtTDP plays a clear role in the repair of topoisomerase I-DNA complexes in Arabidopsis.

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“…11,12 The advantages that plastic embedding have towards conventional frozen and paraffin embedding media include good morphological preservation, thinner section cutting, high-quality section and it avoids technical artifacts. 7,13,14 In this study, the use of plastic embedding allowed thin sections with adequate resolution of structural details for morphometric analysis.…”

Section: Discussionmentioning

confidence: 99%

Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations

et al. 2008

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PURPOSE: This study sought to evaluate the efficiency of glycol methacrylate-embedding medium to detect morphological alterations of human saphenous vein submitted to brief and crescent pressurizations. METHODS: Saphenous veins of 20 CABG patients were randomly distributed into four experimental groups (control, 100, 200 and 300 mmHg pressures during 15 seconds). To quantify the percentage of endothelium spread over vein surface a microscope magnification of 100x was used for measurements. Morphometric analysis was performed using videomicroscopy with the Leica Qwin software in conjunction with a Leica microscope, videocamera, and an on-line computer. RESULTS: A slight tendency of quantitative increase was observed in all parameters including percentage of endothelium spread over vein surface and thickness of saphenous vein walls (intima and media layers). CONCLUSIONS: The glycol methacrylate-embedding allowed sections with adequate resolution of structural details and revealed to be an extremely useful method to study pressurized human saphenous veins.

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Staining methods applied to glycol methacrylate embedded tissue sections

Cited by 98 publications

References 18 publications

Comparison of three different techniques for histological tooth preparation

Comparison of three different techniques for histological tooth preparation

Identification of Tyrosyl-DNA Phosphodiesterase as a Novel DNA Damage Repair Enzyme in Arabidopsis

Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations

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