Staining of serum glycoproteins after electrophoretic separation in acrylamide gels

Keyser, J. W.

doi:10.1016/0003-2697(64)90111-3

Cited by 69 publications

(20 citation statements)

References 5 publications

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“…For the detection of glycosylated proteins in acrylamide gels, the periodic acid-Schiff stain (PAS) method was first introduced in 1964 and by 1969 had been further refined [203,204]. Other modifications made to the PAS staining method have included the capacity for quick detection [205] and applicability to proteins resolved by native PAGE [206].…”

Section: Glycosylated Proteinsmentioning

confidence: 99%

Quantitative proteomics: assessing the spectrum of in-gel protein detection methods

2010

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Proteomics research relies heavily on visualization methods for detection of proteins separated by polyacrylamide gel electrophoresis. Commonly used staining approaches involve colorimetric dyes such as Coomassie Brilliant Blue, fluorescent dyes including Sypro Ruby, newly developed reactive fluorophores, as well as a plethora of others. The most desired characteristic in selecting one stain over another is sensitivity, but this is far from the only important parameter. This review evaluates protein detection methods in terms of their quantitative attributes, including limit of detection (i.e., sensitivity), linear dynamic range, inter-protein variability, capacity for spot detection after 2D gel electrophoresis, and compatibility with subsequent mass spectrometric analyses. Unfortunately, many of these quantitative criteria are not routinely or consistently addressed by most of the studies published to date. We would urge more rigorous routine characterization of stains and detection methodologies as a critical approach to systematically improving these critically important tools for quantitative proteomics. In addition, substantial improvements in detection technology, particularly over the last decade or so, emphasize the need to consider renewed characterization of existing stains; the quantitative stains we need, or at least the chemistries required for their future development, may well already exist.

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Section: Glycosylated Proteinsmentioning

confidence: 99%

Quantitative proteomics: assessing the spectrum of in-gel protein detection methods

2010

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“…In consequence, the molecular mass of F. oxysporum lipase was 30 kDa, being composed of a single polypeptide. By periodic acid-schiff (PAS) staining for detection of sugar chains 11 ) on SDS-PAGE, the F. oxysporum lipase seems to contain a sugar chain (Fig. 2).…”

Section: Molecular Properties Of Lipasementioning

confidence: 99%

Purification and Some Characteristics of Extracellular Lipase fromFusarium oxysporumf. sp.lini

Hashizume

Sasaki

Watanabe

et al. 1992

Bioscience, Biotechnology, and Biochemistry

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“…The buffer system was tris-Na2 EDTA-boric acid (0-1 m), pH 9-2. At the termination of the initial electrophoretic run, slabs were cut from the gel and stained with amido black for protein or developed with periodic acid-Schiff reagent for glycoprotein (Keyser, 1964).…”

Section: Zone Electrophoresismentioning

confidence: 99%

Characterization and Isolation of a Sperm-Coating Antigen From Rabbit Seminal Plasma With Capacity to Block Fertilization

Hunter¹,

Nornes²

1969

Reproduction

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Summary. The relationship between the sperm-coating antigens of rabbit seminal plasma and the phenomenon of decapacitation was studied using agar-gel diffusion, immuno-electrophoresis, chromatography on Sephadex G-200, and polyacrylamide vertical gel electrophoresis.Interpretation of data obtained with these techniques led to the conclusion that a sperm-coating antigen of seminal plasma origin possessed biological activity for blocking fertilization. The sperm-coating antigen was a glycoprotein of approximately 170,000 molecular weight, migrated in an electric field similar to a serum slow \g=b\-globulin and was still present in the seminal fluid of vasectomized males. This sperm\x=req-\ coating antigen was absent from the inactive upper supernatant fluid fraction of seminal plasma after 4 hr of ultracentrifugation at 105,000 g and was present in the active ultracentrifugal pellet.

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Staining of serum glycoproteins after electrophoretic separation in acrylamide gels

Cited by 69 publications

References 5 publications

Quantitative proteomics: assessing the spectrum of in-gel protein detection methods

Quantitative proteomics: assessing the spectrum of in-gel protein detection methods

Purification and Some Characteristics of Extracellular Lipase fromFusarium oxysporumf. sp.lini

Characterization and Isolation of a Sperm-Coating Antigen From Rabbit Seminal Plasma With Capacity to Block Fertilization

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