Staining Similarity of Foot's and Hio<sub>4</sub>-Schiff Technics

Lhotka, John F.; Davenport, H.

doi:10.3109/10520295009110974

Cited by 23 publications

(2 citation statements)

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“…A comparison of tissue sections stained with silver, or by the PAS method, shows a close similarity in the location of the stained features recognized as reticulin (Lhotka & Davenport, 1950;Lhotka & Myhre, 1953), and it has been suggested that a similar binding mechanism operates in each case.…”

Section: Introductionmentioning

confidence: 96%

Staining reticulin in beef dorsal lymph nodes for electron microscopy

Voyle¹

1977

Journal of Microscopy

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Reticulin has been classically identified by virtue of its argyrophilia, as observed by light microscopy. The similiarity between reticulin and collagen has been a matter for considerable discussion. This paper describes the application of a silver-staining procedure which demonstrates the fine structure of these fibres when studied by electron microscopy, giving a direct indication of the morphological identity of the argyrophilic structures, i.e. reticulin, with collagen. Beef lymph node tissue was used for this study.

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Section: Introductionmentioning

confidence: 96%

Staining reticulin in beef dorsal lymph nodes for electron microscopy

Voyle¹

1977

Journal of Microscopy

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“…This would appear to indicate that this particular radical does not play an important part in neurofibrillar silver-tissue affinity. Lhotka and Davenport (1950) suggested that the aldehyde radical might well play an important role in the argyrophilic phenomena since they found that the histological picture obtained with the periodic-acid-Schiff procedure, based on a known aldehyde mechanism, appears identical to that obtained with the Foot ammoniated silver carbonate technic. However, later studies (Lhotka and Myhre, 1953) on the effect of organic oxidants on neurofibrillar argyrophilia did not support this premise and simultaneously a report by Peters (1 953) cast further doubt on an aldehyde-argyfophilic relationship.…”

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confidence: 91%

The Role of the Aldehyde Radical in Neurofibrillar Argyrophilia

Lhotka

1954

Stain Technology

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If tissue aldehydes are blocked prior to silvering in the CajaI and Protargol technics, subsequent neurofibrillar argyrophilia is not appreciably affected. This would appear to indicate that this particular radical does not play an important part in neurofibrillar silver-tissue affinity. Lhotka and Davenport (1950) suggested that the aldehyde radical might well play an important role in the argyrophilic phenomena since they found that the histological picture obtained with the periodic-acid-Schiff procedure, based on a known aldehyde mechanism, appears identical to that obtained with the Foot ammoniated silver carbonate technic. However, later studies (Lhotka and Myhre, 1953) on the effect of organic oxidants on neurofibrillar argyrophilia did not support this premise and simultaneously a report by Peters (1 953) cast further doubt on an aldehyde-argyfophilic relationship.Since the experiments in the oxidation studies were essentially of the positive type, it was felt that it would be of value to continue the study of aldehyde-silver mechanisms in neurofibrillar staining with a series of blocking or negative experiments. It was reasoned that if all tissue aldehydes were blocked prior to silvering in any of the neurofibrillar methods, the relevance of this radical in the staining would be obvious.Two standard types of technics, the Nonidez variation of the Cajal silver nitrate block method (1939) and the silver proteinate (Protargol) method for tissue sections (1947), were selected for investigation, with human, rat and rabbit cerebrum and cerebellum as the experimental tissues. Four aldehyde-blocking reagents, p-nitrophenylhydrazine, 2 4 dinitrophenylhydrazine, hydroxylamine, and 5,5-dimethyl-l,3-cyclohexanedione or dimedone were selected, using the basic reaction specifications of Pearce (1953) (pnitrophenylhydrazine was used the same as 2 4 dinitrophenylhydrazine) but with increased reaction time for the staining in bulk by the Cajal technic. Following the aldehyde-blocking reaction, which was interposed immediately prior to the immersion of the tissue in its respective silver bath, the tissues were thoroughly washed to remove all traces of the blocking reagent. They were then processed according to the appropriate staining technic. Finally, the finished slides of the alde-STAIN TECHNOLOGY, I'OL. 29, NO. 6, NOVExfBER 1954 293 Biotech Histochem Downloaded from informahealthcare.com by McMaster University on 01/05/15 For personal use only. 294 STAIN TECHNOLOGYhyde-blocked tissues were compared microscopically with identical unblocked control tissues as to the intensity of neurofibrillar staining.Regardless of technic or blocking reagent, the results were uniform. In all cases it was impossible to note any discernible difference between the neurofibrillar demonstration in the control sections and in the sections in which all tissue aldehydes had been blocked prior to silvering. It would thus appear that at least as far as neurofibrillar staining is concerned the aldehyde radical does not play an important part in ...

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