Staining Techniques and Microscopy

Dettmeyer, R.

doi:10.1007/978-3-319-77997-3_2

Cited by 4 publications

(5 citation statements)

References 65 publications

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“…Over the decades, histological and immunohistochemical analyses of different post mortem tissues have become increasingly important, as they might provide additional hints that may be useful in determining the time interval after death (Thaik‐Oo et al. ; Dettmeyer, ). For gingival tissue, PMI has been related to different histological changes (Pradeep et al.…”

Section: Introductionmentioning

confidence: 99%

“…Many different approaches have been explored in recent years, and the literature is constantly expanded by newer strategies that are being considered as additional support so that a more accurate value can be provided by using a combination of different methods (Henssege et al 2000a,b). Over the decades, histological and immunohistochemical analyses of different post mortem tissues have become increasingly important, as they might provide additional hints that may be useful in determining the time interval after death (Thaik-Oo et al 2002;Dettmeyer, 2011). For gingival tissue, PMI has been related to different histological changes (Pradeep et al 2009;Yadav et al 2015;Mahalakshmi et al 2016), but the associated post mortem immunohistochemical changes seem to be overlooked.…”

Section: Introductionmentioning

confidence: 99%

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HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

et al. 2018

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Estimating the post mortem interval (PMI) is still a crucial step in Forensic Pathology. Although several methods are available for assessing the PMI, a precise estimation is still quite unreliable and can be inaccurate. The present study aimed to investigate the immunohistochemical distribution and mRNA expression of hypoxia inducible factor (HIF‐1α) in post mortem gingival tissues to establish a correlation between the presence of HIF‐1α and the time since death, with the final goal of achieving a more accurate PMI estimation. Samples of gingival tissues were obtained from 10 cadavers at different PMIs (1–3 days, 4–5 days and 8–9 days), and were processed for immunohistochemistry and quantitative reverse transcription‐polymerase chain reaction. The results showed a time‐dependent correlation of HIF‐1α protein and its mRNA with different times since death, which suggests that HIF‐1α is a potential marker for PMI estimation. The results showed a high HIF‐1α protein signal that was mainly localized in the stratum basale of the oral mucosa in samples collected at a short PMI (1–3 days). It gradually decreased in samples collected at a medium PMI (4–5 days), but it was not detected in samples collected at a long PMI (8–9 days). These results are in agreement with the mRNA data. These data indicate an interesting potential utility of Forensic Anatomy‐based techniques, such as immunohistochemistry, as important complementary tools to be used in forensic investigations.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

et al. 2018

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“…The bone tissues were then dehydrated through a series of ethanol solutions using (TP 1020 semi-closed benchtop tissue processor (Leica, Singapore). The tissues were then embedded in paraffin wax using the standard procedure described by Dettmeyer, [ 37 ]. The specimens were then cut into longitudinal 5 µm serial sections using Reichert-Jung 2045 (MulticutRotary Microtome, Leica Biosystems Nussloch GmbH, Nussloch, Germany).…”

Section: Methodsmentioning

confidence: 99%

Roles of Sodium Hydrogen Exchanger (NHE1) and Anion Exchanger (AE2) across Chondrocytes Plasma Membrane during Longitudinal Bone Growth

Abubakar

Ali²,

Ibrahim³

et al. 2022

Membranes

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Mammalian long bone growth occurs through endochondral ossification, majorly regulated by the controlled enlargement of chondrocytes at the growth plate (GP). This study aimed to investigate the roles of Na+/H+ (sodium hydrogen exchanger (NHE1)) and HCO3− (anion exchanger [AE2]) during longitudinal bone growth in mammals. Bones from P10 SpragueDawley rat pups were cultured exvivo in the presence or absence of NHE1 and AE2 inhibitors to determine their effect on long bone growth. Gross morphometry, histomorphometry, and immunohistochemistry were used to assess the bone growth. The results revealed that the culture of the bones in the presence of NHE1 and AE2 inhibitors reduces bone growth significantly (p < 0.05) by approximately 11%. The inhibitor significantly (p < 0.05) reduces bone growth velocity and the length of the hypertrophic chondrocyte zone without any effect on the total GP length. The total GP chondrocyte density was significantly (p < 0.05) reduced, but hypertrophic chondrocyte densities remained constant. NHE1 fluorescence signaling across the GP length was higher than AE2, and their localization was significantly (p < 0.05) inhibited at the hypertrophic chondrocytes zone. The GP lengthening was majorly driven by an increase in the overall GP chondrocyte and hypertrophic chondrocyte densities apart from the regulatory volume phenomenon. This may suggest that NHE1 and AE2 could have a regulatory role in long bone growth.

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“…The bones were then dehydrated in a series of ethanol solutions using a TP 1020 semi‐closed bench top tissue processor (Leica, Singapore). The bones were then embedded in paraffin wax using standard procedures . Samples were then serially sectioned longitudinally into 4‐5 μm thick sections using a Reichert‐Jung 2045 multicut rotary microtome (USA).…”

Section: Methodsmentioning

confidence: 99%

Postnatal ex vivo rat model for longitudinal bone growth investigations

Abubakar

Ibrahim

Ali

et al. 2019

Anim Models and Exp Med

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Background Chondrocytes in the growth plate ( GP ) undergo increases in volume during different cascades of cell differentiation during longitudinal bone growth. The volume increase is reported to be the most significant variable in understanding the mechanism of long bone growth. Methods Forty‐five postnatal Sprague‐Dawley rat pups, 7‐15 days old were divided into nine age groups (P7‐P15). Five pups were allocated to each group. The rats were sacrificed and tibia and metatarsal bones were harvested. Bone lengths were measured after 0, 24, 48, and 72 hours of ex vivo incubation. Histology of bones was carried out, and GP lengths and chondrocyte densities were determined. Results There were significant differences in bone length among the age groups after 0 and 72 hours of incubation. Histological sectioning was possible in metatarsal bone from all age groups, and in tibia from 7‐ to 13‐day‐old rats. No significant differences in tibia and metatarsal GP lengths were seen among different age groups at 0 and 72 hours of incubation. Significant differences in chondrocyte densities along the epiphyseal GP of the bones between 0 and 72 hours of incubation were observed in most of the age groups. Conclusion Ex vivo growth of tibia and metatarsal bones of rats aged 7‐15 days old is possible, with percentage growth rates of 23.87 ± 0.80% and 40.38 ± 0.95% measured in tibia and metatarsal bone, respectively. Histological sectioning of bones was carried out without the need for decalcification in P7‐P13 tibia and P7‐P15 metatarsal bone. Increases in chondrocyte density along the GP influence overall bone elongation.

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Staining Techniques and Microscopy

Cited by 4 publications

References 65 publications

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

Roles of Sodium Hydrogen Exchanger (NHE1) and Anion Exchanger (AE2) across Chondrocytes Plasma Membrane during Longitudinal Bone Growth

Postnatal ex vivo rat model for longitudinal bone growth investigations

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