In this study, antimicrobial and electromagnetic shielding properties of metal composite single jersey fabrics were investigated. For this purpose, stainless steel wire, copper wire, and metalized silver polyamide filament were commingled with textured polyester. The composite yarns were knitted to single jersey fabrics. Electromagnetic shielding effectiveness of samples was measured according to free space test method between 0.8 and 5.2 GHz frequency range. In addition, antibacterial and antifungal activity tests were performed for the overall technical characterization of composite fabrics. The effects of metal type, filament form on electromagnetic shielding effectiveness, and antimicrobial properties were examined. All composite fabrics showed electromagnetic shielding properties at different levels up to 35 dB in vertical direction. Fabrics including metalized multifilament yarn exhibit higher electromagnetic shielding effectiveness in both vertical and horizontal directions. In addition, antibacterial activity level of metalized silver polyamide and copper composite fabrics reached up to 99% against Klebsiella pneumoniae and Staphylococcus aureus. Furthermore, Aspergillus niger could not grow on these composite fabrics.