Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis<i>ZINC FINGER PROTEIN2</i>

Cai, Suqin; Lashbrook, Coralie C.

doi:10.1104/pp.107.110908

Cited by 204 publications

(257 citation statements)

References 137 publications

(162 reference statements)

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“…There is a large dynamic range of HAE expression through the process of abscission because HAE progressively increases 27-fold from stage-12 to -15 abscission zones (Fig. 1F) (16). This study also helps explain the large dynamic range of gene expression that is observed in the process of abscission.…”

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confidence: 61%

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Floral organ abscission is regulated by a positive feedback loop

Patharkar

Walker

2015

Proc. Natl. Acad. Sci. U.S.A.

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Abscission is the process by which plants shed unwanted organs, either as part of a natural developmental program or in response to environmental stimuli. Studies in Arabidopsis thaliana have elucidated a number of the genetic components that regulate abscission of floral organs, including a pair of related receptor-like protein kinases, HAESA and HAESA-like 2 (HAE/HSL2) that regulate a MAP kinase cascade that is required for abscission. HAE is transcriptionally up-regulated in the floral abscission zone just before cell separation. Here, we identify AGAMOUS-like 15 (AGL15; a MADS-domain transcription factor) as a putative regulator of HAE expression. Overexpression of AGL15 results in decreased expression of HAE as well as a delayed abscission phenotype. Chromatin immunoprecipitation experiments indicate that AGL15 binds the HAE promoter in floral receptacles. AGL15 is then differentially phosphorylated through development in floral receptacles in a MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4/5-dependent manner. MAP kinase phosphorylation of AGL15 is necessary for full HAE expression, thus completing a positive feedback loop controlling HAE expression. Together, the network components in this positive feedback loop constitute an emergent property that regulates the large dynamic range of gene expression (27-fold increase in HAE) observed in flowers when the abscission program is initiated. This study helps define the mechanisms and regulatory networks involved in a receptor-mediated signaling pathway that controls floral organ abscission.A bscission is the process that plants use to shed unwanted organs. Various plants can abscise leaves, fruits, and flowers. One of the most noticeable abscission events occurs when deciduous trees and shrubs shed their leaves in the fall. Plants can abscise organs as part of a developmental program or in an inducible manner in response to stimuli like abiotic or biotic stress. For example, tomatoes can abscise leaves and flowers in response to drought or insect feeding (1-3). In order for abscission to occur, a layer of small and cytoplasmically dense cells, known as an abscission zone, must be laid down during development at the boundary between the organ to be abscised and the body of the plant (4).Arabidopsis thaliana has been used to elucidate a number of the molecular and genetic components that regulate abscission of floral organs. In Arabidopsis, floral organs abscise shortly after pollination, which corresponds to stage-16 flowers or approximately floral position 4-6, where anthesis is defined as position 1 and older flowers are defined as increasing numerical positions ( Fig. 1 A and B) (5). A number of genetic components regulating this abscission process have been described thoroughly in recent reviews (6, 7). In brief, a pair of related receptor-like protein kinases, HAESA and HAESA-like 2 (HAE/HSL2), are required for floral abscission (Fig. 1C) and are thought to be triggered by a peptide derived from INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) (8, 9). A mitogen-act...

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confidence: 61%

“…HAE is transcriptionally up-regulated in the floral abscission zone just before cell separation ( Fig. 1 D-F) (15,16). The initial goal of this study was to extend the known floral abscission pathway by identifying upstream components that regulate HAE expression.…”

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confidence: 99%

Floral organ abscission is regulated by a positive feedback loop

Patharkar

Walker

2015

Proc. Natl. Acad. Sci. U.S.A.

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“…ZFP1 was previously implicated in photomorphogenic responses, although the precise function of this gene was not revealed (Chrispeels et al, 2000). ZFP2 was shown to control floral abscission, as overexpressing plants enhanced the retention of floral organs (Cai and Lashbrook, 2008). ZFP5, ZFP6, and ZFP8 were shown to regulate the differentiation of trichomes through integrating GA and cytokinin signaling (Zhou et al, 2011(Zhou et al, , 2013.…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

et al. 2014

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Seed germination is controlled by environmental signals, including light and endogenous phytohormones. Abscisic acid (ABA) inhibits, whereas gibberellin promotes, germination and early seedling development, respectively. Here, we report that ZFP3, a nuclear C2H2 zinc finger protein, acts as a negative regulator of ABA suppression of seed germination in Arabidopsis (Arabidopsis thaliana). Accordingly, regulated overexpression of ZFP3 and the closely related ZFP1, ZFP4, ZFP6, and ZFP7 zinc finger factors confers ABA insensitivity to seed germination, while the zfp3 zfp4 double mutant displays enhanced ABA susceptibility. Reduced expression of several ABA-induced genes, such as RESPONSIVE TO ABSCISIC ACID18 and transcription factor ABSCISIC ACID-INSENSITIVE4 (ABI4), in ZFP3 overexpression seedlings suggests that ZFP3 negatively regulates ABA signaling. Analysis of ZFP3 overexpression plants revealed multiple phenotypic alterations, such as semidwarf growth habit, defects in fertility, and enhanced sensitivity of hypocotyl elongation to red but not to far-red or blue light. Analysis of genetic interactions with phytochrome and abi mutants indicates that ZFP3 enhances red light signaling by photoreceptors other than phytochrome A and additively increases ABA insensitivity conferred by the abi2, abi4, and abi5 mutations. These data support the conclusion that ZFP3 and the related ZFP subfamily of zinc finger factors regulate light and ABA responses during germination and early seedling development.

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“…About peroxidases with some degree of expression in flower in the phylogenetic tree, the most related ones was AtPrx53 of A. thaliana in sub-group A2 that shared 75% identity and 86% similarity with CavPrx. AtPrx53 was described to be expressed in the stamen abscission zone by microarray analysis [31]. This peroxidase is not flower-specific because it resulted expressed also in vascular bundles [35] and it could be putatively involved in lignification [3].…”

Section: Discussionmentioning

confidence: 99%

“…It showed a Poly A tail, with a T nucleotide upstream, as we expected using H-T 11 A oligo(dT) primer. After alignment with sequences deposited in NCBI database, the fragment showed a high level of identity (85%) and similarity (92%) with the gene AtPrx53 encoding for a class III peroxidase of A. thaliana expressed in flower tissues [31].…”

Section: Isolation Of a Gene Sequence Predominantly Expressed In Matumentioning

confidence: 99%

Isolation of a gene encoding for a class III peroxidase in female flower of Corylus avellana L.

et al. 2012

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Hazelnut is a monoecius species characterized by mid-winter blooming and sporophytic incompatibility. The molecular mechanisms at the basis of the female flower development and of the pollen-stigma interaction are little known, although pollination in this species is a critical factor to ensure good yield. Differential Display technique was used to study genes expressed during the female flower development, comparing styles before emergence from the bud and styles at full bloom. The full-length cDNA clone, designated CavPrx (Corylus avellana peroxidase) and isolated in mature styles, was characterized as a sequence encoding for a 330 amino acids protein, containing all the conserved features of class III peroxidases. CavPrx resulted expressed only in styles, with a peak in mature styles pollinated with compatible pollen. Class III peroxidases are expressed in several different plant tissue types and are involved in a broad spectrum of physiological processes. Until now, four peroxidases expressed in the stigma were identified in Arabidopsis thaliana and Senecio squalidus: they were assumed to be possibly involved in pollen-pistil interaction, pollen tube penetration/growth and/or in defence against pathogens. CavPrx is the first gene for a floral peroxidase isolated in hazelnut and its expression pattern suggests a possible role in the pollination process.

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Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing ArabidopsisZINC FINGER PROTEIN2

Cited by 204 publications

References 137 publications

Floral organ abscission is regulated by a positive feedback loop

Floral organ abscission is regulated by a positive feedback loop

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

Isolation of a gene encoding for a class III peroxidase in female flower of Corylus avellana L.

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