Standard addition method applied to the urinary quantification of nicotine in the presence of cotinine and anabasine using surface enhanced Raman spectroscopy and multivariate curve resolution

Biological and Medical Applications of Multivariate Curve Resolution Assisted Raman Spectroscopy

“…Poppi lab has utilized this strategy to identify and possibly quantify different metabolites. [94][95][96][97] A quantitative method for an on-site determination of uric acid in urine using gold nanoparticle coated paper substrate for SERS detection has been suggested. 96 Also, by using a similar nanoparticle coated substrate, a procedure for the determination of the antibiotic moxifloxacin in urine has been demonstrated.…”

Bioanalytical applications of surface-enhanced Raman spectroscopy: de novo molecular identification

“…139 With the use of standard addition, the nicotine metabolites were detected in pure solutions, mixtures, and human urine. They used multivariate curve resolution-alternating least squares (MCR-ALS) to compensate for the matrix affects from the urine to be able to quantify the nicotine without separation.…”

Methodologies for assessment of limit of detection and limit of identification using surface-enhanced Raman spectroscopy

“…As the number of analytes increases, it is appropriate to introduce selectivity in the SERS methodology, which commonly is supported by powerful algorithms and chemometric based analyses. A remaining challenge is to introduce sufficient selectivity to resolve signals from an interfering matrix, as for example for drugs in saliva [16][17][18] or antibiotic substances [19] and nicotine [20] in urine. The first step towards this goal is to obtain SERS substrates that are giving rise to reliable, reproducible and satisfying detection limits in the analytical set-up.…”