2023
DOI: 10.1016/j.actbio.2022.08.021
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Standard in vitro evaluations of engineered bone substitutes are not sufficient to predict in vivo preclinical model outcomes

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Cited by 7 publications
(4 citation statements)
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“…When studying new biomaterials/surfaces for bone regeneration, promising in vitro results do not often correlate with the outcome obtained from the in vivo experiments. 17 Nevertheless, new regenerative materials and approaches need to be assessed through reliable and comparable methods for rapid translation to the clinic. There is a considerable need for proven in vitro assays that can reduce the burden on animal testing by allowing assessment of biomaterial utility predictive of the results currently obtained through in vivo studies.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…When studying new biomaterials/surfaces for bone regeneration, promising in vitro results do not often correlate with the outcome obtained from the in vivo experiments. 17 Nevertheless, new regenerative materials and approaches need to be assessed through reliable and comparable methods for rapid translation to the clinic. There is a considerable need for proven in vitro assays that can reduce the burden on animal testing by allowing assessment of biomaterial utility predictive of the results currently obtained through in vivo studies.…”
Section: Introductionmentioning
confidence: 99%
“…There is a considerable need for proven in vitro assays that can reduce the burden on animal testing by allowing assessment of biomaterial utility predictive of the results currently obtained through in vivo studies. 17 Our group has been working in developing new in vitro test to characterize the biocompatibility 18,19 and regenerative potential 20 of different types of biomaterials based on proteomic evaluations. This technique was previously employed to evaluate the effect of Ca 2+ on protein adsorption onto dental implant surfaces.…”
Section: Introductionmentioning
confidence: 99%
“…In vitro evaluation of scaffolds is subjected to several limitations arising from the reduced complexity of the in vitro culture models, the absence of immune or inflammatory response as well as the impossibility to reproduce the complex cascade of events occurring after in vivo implantation [ 11 , 12 ]. These include the interaction with body fluids (e.g.…”
Section: Introductionmentioning
confidence: 99%
“…In vitro osteogenesis of MSCs serves as initial step in this validation process ( Bongio et al, 2015 ). The traditional in vitro induction of osteogenesis requires medium supplementation with a differentiation cocktail that includes dexamethasone, ascorbic acid and organic β-glycerophosphate (BGP) ( Hatt et al, 2022b ). This osteogenic cocktail induces cell morphology changes, increases alkaline phosphatase (ALP) activity, the expression of osteogenic gene markers and secretion of matrix minerals ( Langenbach and Handschel, 2013 ; Zha et al, 2022 ).…”
Section: Introductionmentioning
confidence: 99%