Standardised extract of safed musli (Chlorophytum borivilianum) increases aphrodisiac potential besides being safe in male Wistar rats

Abstract

The standardised extract of root of safed musli (Chlorophytum borivilianum) was evaluated for its aphrodisiac potential and safety profile on reproductive system. Wistar albino rats were trained to provide sexual experience under a dim red light (10 W) in a glass tank. Male and female rats were placed periodically in the glass tank in a particular order, that is male followed by introduction of the receptive female. Dosing of extract was carried out for 54 days at 125 and 250 mg kg p.o to male rats. On 14th and 28th days, the animals were observed from the cage side for sexual behaviours. Safed musli at both dose levels enhanced sexual vigour and libido which might be useful for treatment of sexual dysfunction in male till 28th day. Safety profile was assessed after 54 days of drug treatment, where both doses showed an increase in sperm count and increase in sperm motility. Thus, it can be stated that both doses possessed the spermatogenic potential, which would be highly beneficial in treating oligospermia or low sperm count. After 54 days of study, there was increase in sperm abnormality (%) at both doses, but not more than 10%, which indicated that this formulation will not induce infertility.

Chemically characterised extract ofSaraca asocaimproves the sexual function in male Wistar rats

“…Up to 28 days of study, sildenafil showed significant improvement in all sexual functions, that is increase in MF and IF and decrease in ML and IL as was found by Das et al. (). At 42 and 54 days of the study, a significant increase in ML and IL and a significant decrease in MF and IF of the standard/sildenafil‐treated group were observed when compared to normal control group.…”

“…The selection of male and female ratio for the following model is a crucial aspect. Males and females were taken in a ratio of 1:1 (Das et al., ).…”

“…One to two drops of the obtained cell suspension was placed onto the slide and observed under a light microscope. Different phases of oestrous cycle were identified based on the presence of three types of cells; round epithelial cells with nucleus indicating the pro‐oestrus phase, irregular non‐nucleated cornified epithelial cells indicating the presence of oestrus phase and predominant leucocytes (round in shape) indicating the di‐oestrus phase (Das et al., ).…”

“…After allowing the spermatozoa to diffuse into the medium for about 1 min, the tissue was removed, and counting of spermatozoa was carried out. Improved Neubauer chamber was used for counting (Das et al., ). Sperm concentration (expressed as 10 6 spermatozoa/ml) is the number of spermatozoa ( N ) divided by the volume in which they are found, that is the volume of the total number of rows ( n ) examined for the replicates (20 nl each for grids 4, 5, 6) multiplied by the dilution factor used to prepare the sample, that is…”

“…Using 10% formalin solution, the isolated testes of all rats were fixed and embedded in paraffin followed by sectioning and staining using haematoxylin–eosin stain. Microscopic examination of the slides was performed to see any histopathological changes (Das et al., ).…”