Standardised herbal extract of chlorogenic acid from leaves of Etlingera elatior (Zingiberaceae)

Abstract

Background:Chlorogenic acid (CGA) or 5-caffeoylquinic acid, was found to be the dominant phenolic compound in leaves of Etlingera elatior (Zingiberaceae). The CGA content of E. elatior leaves was significantly higher than flowers of Lonicera japonica (honeysuckle), the commercial source. In this study, a protocol to produce a standardised herbal CGA extract from leaves of E. elatior using column chromatography was developed.Materials and Methods:Freeze-dried leaves of E. elatior were extracted with 30% ethanol, and sequentially fractionated using Diaion HP-20 and Sephadex LH-20.Results:The CGA fractions, which yielded extracts of 10% and 40% w/w purity, possessed antioxidant, tyrosinase inhibition, and antibacterial properties. The entire fractionation process took only 6.5 hours, using gravity flow. From 50 g of leaves, the final yield of CGA extract was 0.2 g (0.4%). The CGA content of the standardised herbal extract from leaves of E. elatior (40%) is 1.6 times that of commercial extracts from honeysuckle flowers (25%).Conclusion:With high CGA content, the standardised herbal extract has a great potential to be developed into functional food and other health products. Leaves of E. elatior, which currently have no economic value, could serve as an alternative source of CGA. Leaves are large, available in abundance, and harvesting is non-destructive to the plants.

Mammillaria Species—Polyphenols Studies and Anti-Cancer, Anti-Oxidant, and Anti-Bacterial Activities

“…Chlorogenic acid is the ester of caffeic acid and is much more common than gentisic acid. Chlorogenic acid was isolated from different plants species such as Etlingera elatior leaves, coffee, and fruit [27] as well as medicinal plants [17]. The chlorogenic acid is known for strong its anti-oxidant, anti-cancer, anti-inflammatory, and antiviral properties, and modulates the anti-oxidant enzyme activities [28,29].…”

Antioxidant properties of herbs with enhancement effects of drying treatments: A synopsis

“…A protocol to produce a standardised extract of CGA with 40% w/w purity from freeze-dried leaves of E. elatior has been developed. 38 Freeze-drying of E. elatior leaves followed by extraction with 30% ethanol, and sequential fractionation using Diaion HP-20 and Sephadex LH-20 yielded a CGA extract with 40% w/w purity. CGA fractions had antioxidant, antibacterial and antityrosinase properties.…”

Aldose reductase inhibitor form Cassia glauca: A comparative study of cytotoxic activity with Ag nanoparticles (NPs) and molecular docking evaluation

“…All fractions, sub-fractions, and pure compounds were screened by TLC using ethyl acetate (100): formic acid (11): acetic acid (11): water (20) on pre-coated silica gel 60 on aluminum sheets (E-Merck, Darmstadt, Germany). NMR spectra ( 1 H and 13 C APT) were measured using JEOL JNM-ECA (400 MHz, DMSO- d 6 ) (Faculty of Pharmacy, Ain Shams University, Egypt) while, ESI-MS was performed using Micromass Quattro II (Ruprecht-Karls-Universität, Heidelberg, Germany) [ 26 – 28 ]. The samples were dissolved in DMSO, run at room temperature, and chemical shifts were given as δ ppm relative to tetramethylsilane (TMS) as an internal standard.…”