Standardization and control of meningococcal vaccines, group A and group C polysaccharides

Wong, K. H.; Barrera, O; Sutton, Ann; May, Joan C.; Hochstein, D; Robbins, J B; Robbins, John B.; Parkman, Paul D.; Seligmann, Edward B.

doi:10.1016/s0092-1157(77)80005-x

Cited by 87 publications

(45 citation statements)

References 19 publications

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“…In the 20 immunized human volunteers, no systemic reactions were observed and local reactions were mild, transient, and infrequent (15%). 19 (95%) responded with >1.0 g/ml increases in specific serum antibody. The response rate to immunization with type II polysaccharide in those individuals who had low preimmunization antibody levels (92%) was greater than that reported in adults with low levels of antibody to type III polysaccharide before immunization with that vaccine (73%) (25).…”

Section: Discussionmentioning

confidence: 99%

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Immunochemical analysis and immunogenicity of the type II group B streptococcal capsular polysaccharide.

Kasper¹,

Baker²,

Galdes³

et al. 1983

J. Clin. Invest.

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A B S T R A C T The relationship between group B streptococcal (GBS) type-specific antisera and the type II-specific polysaccharide is evaluated from a structural and immunologic viewpoint. Although all GBS type-specific polysaccharides are composed of the same monosaccharides, the type II antigen is more complex structurally and contains these sugars in a molar ratio different from the other antigens. Type II polysaccharide has two side chains. One contains only sialic acid and is less susceptible to acid cleavage than sialic acid residues found on types III, Ia, and Ib polysaccharides. The other side chain is composed of galactose as the only sugar. Immunochemical studies demonstrate that the type II polysaccharide has several immunodeterminants. One of these determinants is likely to be the side-chain galactose, while sialic acid appears to comprise part of another immunodeterminant, more complex than sialic acid alone. A series of cross-reactions is demonstrated between the type II native antigen and antisera to serotypes Ia, III, and Ib by a sensitive radioactive antigen-binding assay, which account for additional, complex immunodeterminants. The strongest of these cross-reactions is with type Ia antiserum and the weakest with lb antiserum. Since Ia and lb polysaccharides differ in only one linkage, these findings suggest that the trisaccharide BD-

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Section: Discussionmentioning

confidence: 99%

“…1 (18). Therefore, this constant can be used for comparative estimates of molecular size of polysaccharides (19).…”

Section: Introductionmentioning

confidence: 99%

Immunochemical analysis and immunogenicity of the type II group B streptococcal capsular polysaccharide.

Kasper¹,

Baker²,

Galdes³

et al. 1983

J. Clin. Invest.

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“…Purified serogroup A N. meningitidis capsular PS was obtained from Aventis Pasteur-USA, Swiftwater, Pa. Molecular size was estimated using a Sepharose CL-4B gel filtration column eluted with a sodium acetate buffer (31). The degree of O acetylation was assessed by proton nuclear magnetic resonance (NMR) (14).…”

Section: Methodsmentioning

confidence: 99%

Effect of O Acetylation of Neisseria meningitidis Serogroup A Capsular Polysaccharide on Development of Functional Immune Responses

et al. 2002

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“…In the latter instance, the sample was first equilibrated in 3% DOC, 0.01 M EDTA, and 0.05 M glycine, pH 8.8. Determination of the partition coefficient, Kd, was done as described by Wong et al (25).…”

Section: D)mentioning

confidence: 99%

Complex of Meningococcal Group B Polysaccharide and Type 2 Outer Membrane Protein Immunogenic in Man

Zollinger¹,

Mandrell²,

Griffiss³

et al. 1979

J. Clin. Invest.

194

118

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A B S T R A C T A noncovalent complex of meningococcal group B polysaccharide and type 2 outer membrane protein has been characterized and its potential as a vaccine against group B meningococcal disease investigated. The polysaccharide component was found to have a partition coefficient, Kd, of 0.34 on Sepharose CL-4B in the presence of sodium deoxycholate. The protein consisted of four to five major proteins including the principal outer membrane protein. Hydrophobic binding between the protein and polysaccharide was demonstrated by gel filtration and isopycnic CsCl density gradient centrifugation and found to involve all of the proteins. After demonstrating safety and immunogenicity in animals, two lots of vaccine were tested in a total of eight volunteers. Two 120-,ug doses were given subcutaneously at 0 and 5 wk. Mild local reactions occurred in all eight volunteers, but no systemic reactions were observed. 2 wk after the first dose, six of the volunteers had increased levels of bactericidal antibodies against both the group B polysaccharide and the outer membrane proteins. Antibody rises to the group B polysaccharide (mean 6-fold) were confirmed by passive hemagglutination assays and rises to the proteins (mean 10-fold) by a solid phase radioimmunoassay. The second dose resulted in little or no increase in antibody titers. Antibody titers declined over a period of 14 wk but mostly remained above preimmunization levels. Bactericidal antibodies with specificity for the group B polysaccharide were mostly of the immunoglobulin (Ig)M class, and were directed against a determinant associated only with high molecular weight polysaccharides. We conclude that both the group B polysaccharide and the outer membrane protein are immunogenic in man when presented as a complex and that the complex warrants further testing and development as a vaccine against group B meningococcal disease.

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Standardization and control of meningococcal vaccines, group A and group C polysaccharides

Cited by 87 publications

References 19 publications

Immunochemical analysis and immunogenicity of the type II group B streptococcal capsular polysaccharide.

Immunochemical analysis and immunogenicity of the type II group B streptococcal capsular polysaccharide.

Effect of O Acetylation of Neisseria meningitidis Serogroup A Capsular Polysaccharide on Development of Functional Immune Responses

Complex of Meningococcal Group B Polysaccharide and Type 2 Outer Membrane Protein Immunogenic in Man

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