Standardization and formulation of an herbal appetite-stimulating drug from Gentiana olivieri

Azadbakht, Mohammad; Nayebi, Elham; Fard, Reza Enayati; Khaleghi, Fatemeh

doi:10.1016/j.hermed.2019.100306

Cited by 6 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the increase in the body weight in Salix 200 and Salix 300 treatments might be due to the synergistic effect of salicin, caffeic acid, other polyphenols present in S. alba extract which could stimulate increased digestion and metabolism of nutrients, causing greater efficiency in the utilization of feed which results in enhanced growth in chickens. Our results are in agreement with the earlier findings (Hernandez et al 2004;Azadbakht et al 2020) where the plant extract had appetite and digestion stimulating properties. Moreover, due to the antioxidant property of S. alba it may eliminate the production of free radicals which ultimately reduces the oxidative stress in chickens and improves their growth performance (Nahrstedt et al 2007).…”

Section: Discussionsupporting

confidence: 94%

Effect of Salix alba leave extract on growth performance, antioxidant level and immune status of broiler chickens reared at high altitude cold desert

et al. 2021

View full text Add to dashboard Cite

Extremes of climate and hypobaric hypoxia cause poor growth performance in broiler chickens at high altitude and the present study investigates the effect of aqueous extract of Salix alba leaves on growth performance, antioxidant level and immune status of broiler chickens reared at high altitude cold desert (3500 m). High performance liquid chromatography (HPLC) analysis of the extract revealed the presence of salicin and caffeic acid. In vitro efficacy evaluation revealed that the extract had a significant cytoprotective effect on lymphocytes. For in-vivo study, a total of 105 one d old RIR cross-bred broiler chickens were randomly assigned to seven groups: 1) Control fed basal diet and 2) Six treatments fed basal diet + S. alba extract @100, 150, 200, 300, 400, and 800 mg kg −1 body weight of chicken, respectively. Chickens in the Salix 300 group had greater body weight (p < 0.05) and improved feed conversion ratio (FCR) compared to other treatments. Furthermore, chickens had greater (p < 0.05) total antioxidant capacity (TAC), free radical scavenging activity, interleukin-2, and lower malondialdehyde (MDA), interleukin-1, and interleukin-6 concentration as compared to the control group. Net profit was also increased in all the treatments that were fed diet with S. alba extract due to less mortality in chickens. These findings indicated that inclusion S. alba extract at dose concentration of 300 mg kg −1 body weight of chicken, exhibited a beneficial effect on growth performance through immunomodulation and up-regulation of antioxidants, and therefore, it has a potential to be used as a phytogenic feed additive for improving broiler performance at high altitude.

show abstract

Section: Discussionsupporting

confidence: 94%

Effect of Salix alba leave extract on growth performance, antioxidant level and immune status of broiler chickens reared at high altitude cold desert

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The decrease of the surfactant content will reduce the surfactant solubilizing ability and weaken the self-microemulsifying ability, resulting in an increase in particle size. The larger particle size may be also due to the easily aggregation of the particles when dispersed in pH 6.8 PBS, with time increasing [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

Preparation, Characterization, and In Vivo Evaluation of Gentiopicroside-Phospholipid Complex (GTP-PC) and Its Self-Nanoemulsion Drug Delivery System (GTP-PC-SNEDDS)

et al. 2023

View full text Add to dashboard Cite

The objective of the present study was to develop a gentiopicroside-phospholipid complex (GTP-PC) and its self-nanoemulsion drug delivery system (GTP-PC-SNEDDS) to increase the oral bioavailability of gentiopicroside (GTP). The factors affecting the formation of GTP-PC were studied with the complexation efficiency and dissociation rate. The properties of the complex were investigated by means of differential scanning calorimetry (DSC), X-ray diffraction (XRD), Fourier transform infrared spectra (FT-IR), dissolution, etc. Then, GTP-PC was loaded into SNEDDS by investigating the effects of weight ratios of GTP-PC to blank SNEDDS, preparation technology, dilution media, and dilution multi, based on the screening results of oils, surfactants, and cosurfactants. In rats, GTP, GTP-PC, and GTP-PC-SNEDDS were orally administered at different times, and GTP concentrations were determined using RP-HPLC. The optimal GTP-PC was prepared with tetrahydrofuran as the reaction solvent, GTP:phospholipid = 1:2, and stirring for 4 h. The optimal prescription for GTP-PC-SNEDDS was as follows: Maisin 35-1:Miglycol = 30%, Labrasol:Cremophor EL = 1:4 = 40%, Transcutol P = 30%; Maisin 35-1:Miglycol = 12, and the ratio of GTP-PC to blank was 1:10—then the mixture was stirred at 37 °C for 1 d and then placed for 2 d to form stable GTP-PC-SNEDDS. After oral administration of GTP, GTP-PC and GTP-PC-SNEDDS, and mean plasma GTP concentration–time curves were all in accordance with the single-compartment model. The Cmax, AUC0-∞, and Fr of the three formulations were significantly higher than that of GTP, demonstrating that GTP was metabolized rapidly, and its higher bioavailability could be achieved by the formation of GTP-PC and GTP-PC-SNEDDS. Among the three formations, the bioavailability of GTP-PC-SNEDDS was highest, with approximately 2.6-fold and 1.3-fold of Fr value, compared with GTP-PC (suspension) and GTP-PC (oil solution), respectively. Compared with GTP, GTP-PC and GTP-PC-SNEDDS enhanced the bioavailability of GTP significantly. In the future, this study could serve as a reference for clinical trials using GTP-PC and GTP-PC-SNEDDS.

show abstract

“…The effectiveness and safety in using herbal products are closely related to the standardization of the plant extracts. Standardization of the extract is an evaluation parameter used for quality assurance during the development and production of herbal medicines (Azadbakht et al, 2020). The standardization process involves different approaches, ranging from the pre-treatment of plant material, including the extraction operation (choice of solvents, extraction methods, and operational conditions), to the development and validation of analytical methods suitable for the quantification of phytochemical markers (Khan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical profile, in vitro activities, and toxicity of optimized Eugenia uniflora extracts

Almeida¹,

Santos²,

Machado³

et al. 2023

BLACPMA

View full text Add to dashboard Cite

In this study, we investigated the influence of mixture design on the chemical profile of Eugenia unifloraleaves, evaluating the antioxidant and antimicrobial activities, the toxic and hemolytic potential, with the focus on the improvement of the polyphenol’s extraction for incorporation of the extract in semi-solid forms with antifungal action. The chemical analysis was evaluated by UV-Vis and HPLC. The antimicrobial, antioxidant, and hemolytic activities were monitored. The flavonoid content ranged from 2.63-7.98 %w/w and tannins from 5.42-18.29 %w/w. The extract consisted of gallic acid (0.09-1.29%; w/w), ellagic acid (0.09-0.37%; w/w), and myricitrin (0.18-1.20%; w/w). The most successful solvent system with the highest level of active extract was water: ethanol: propylene glycol. The extracts showed fungicidal properties (3.9 μg/mL), high antioxidant activity (IC50: 9.50 μg/mL), and low toxicity. These solvent mixtures can improve the in vitro bioactivities when compared to pure solvents and this result demonstrates the importance of mixture designs as useful tools for creating high-quality herbal products and elucidate the potential of E. unifloraglycolic extracts as active herbal pharmaceutical ingredients in topical delivery systems.

show abstract

Standardization and formulation of an herbal appetite-stimulating drug from Gentiana olivieri

Cited by 6 publications

References 19 publications

Effect of Salix alba leave extract on growth performance, antioxidant level and immune status of broiler chickens reared at high altitude cold desert

Effect of Salix alba leave extract on growth performance, antioxidant level and immune status of broiler chickens reared at high altitude cold desert

Preparation, Characterization, and In Vivo Evaluation of Gentiopicroside-Phospholipid Complex (GTP-PC) and Its Self-Nanoemulsion Drug Delivery System (GTP-PC-SNEDDS)

Phytochemical profile, in vitro activities, and toxicity of optimized Eugenia uniflora extracts

Contact Info

Product

Resources

About