Standardization and phytochemical screening of Centella asiatica, Moringa oleifera L. leaf and Spirulina sp. simplicia

Abstract

Centella asiatica, Spirulina sp., Moringa oleifera have been used for traditional medicine. Studying the phytochemical constituents helps to reveal the usage of plants used as lead compounds for active pharmaceutical ingredients in commercial purposes. This study aims to determine the standardization and screening of phytochemicals from CA, ME and Spirulina. Standardization of CA, ME and spirulina showed as follows: total water 5.29 ± 1.1%; 5.98 ± 0.006% and 8.64 ± 1.16, total water soluble simplicia: 19.7± 0.57%; 10.97 ± 0.99 and 10.98 ± 1%, total ethanol soluble simplicia: 22.3 ± 0.57%; 15.99 ± 1.73% and 39.92 ± 1.73%, total ash: 23.11 ± 0.56%; 8.15 ± 0.28% and 8.65 ± 0.28%, total ash insoluble acid: 0.42 ± 0.07%; 2.15 ± 0.28% and 0.66 ± 0.28% respectively. Both simplicia of ME and spirulina contained of alkaloid, tannin, saponin, triterpene/steroid, flavonoid and glycoside whereas CA simplicia contained tannin, saponin, triterpene/steroid, flavonoid and glycoside.