Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue

Debnath, Tanya; Chelluri, Lakshmi Kiran

doi:10.1016/j.htct.2018.05.001

Cited by 41 publications

(30 citation statements)

References 26 publications

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“…Some studies showed different cell line stabilities in cell phenotype and functional characteristics from P10 up to P30, regarding (54,55) and genomic stability from P5 to P13 (52,53,56). We used cells in a lower passage based on a previous literature that described genomic stability in lower passages and the use of all cells in a similar passage for experimental homogeneity.…”

Section: Discussionmentioning

confidence: 99%

Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo

et al. 2020

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Mammary tumors affect intact and elderly female dogs, and almost 50% of these cases are malignant. Cell culture offers a promising preclinical model to study this disease and creates the opportunity to deposit cell lines at a cell bank to allow greater assay reproducibility and more reliable validation of the results. Another important aspect is the possibility of establishing models and improving our understanding of tumor characteristics, such as vasculogenic mimicry. Because of the importance of cancer cell lines in preclinical models, the present study established and characterized primary cell lines from canine mammary gland tumors. Cell cultures were evaluated for morphology, phenotype, vasculogenic mimicry (VM), and tumorigenicity abilities. We collected 17 primary mammary carcinoma and three metastases and obtained satisfactory results from 10 samples. The cells were transplanted to a xenograft model. All cell lines exhibited a spindle-shaped or polygonal morphology and expressed concomitant pancytokeratin and cytokeratin 8/18. Four cell lines had vasculogenic mimicry ability in vitro, and two cell lines showed in vivo tumorigenicity and VM in the xenotransplanted tumor. Cellular characterization will help create a database to increase our knowledge of mammary carcinomas in dogs, including studies of tumor behavior and the identification of new therapeutic targets.

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Section: Discussionmentioning

confidence: 99%

Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo

et al. 2020

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“…The number of stem/progenitor cells that can be obtained for use in BTE construct preparation is often limited by the amount of donor material that can be harvested, usually from bone marrow or adipose tissue. While possible, in vitro expansion of donor cells, to reach adequate numbers for therapeutic doses, is not an optimal solution, as it is time consuming and can negatively impact stem cell "quality" [35]. Although EStim has mainly been shown to increase the rate of cell proliferation, contrary findings exist, that show EStim can also decrease, or have no effect on cell proliferation (for general review refer to [36]).…”

Section: Cell Proliferation and Apoptosismentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

162

148

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Electrical stimulation (EStim) has been shown to promote bone healing and regeneration both in animal experiments and clinical treatments. Therefore, incorporating EStim into promising new bone tissue engineering (BTE) therapies is a logical next step. The goal of current BTE research is to develop combinations of cells, scaffolds, and chemical and physical stimuli that optimize treatment outcomes. Recent studies demonstrating EStim's positive osteogenic effects at the cellular and molecular level provide intriguing clues to the underlying mechanisms by which it promotes bone healing. In this review, we discuss results of recent in vitro and in vivo research focused on using EStim to promote bone healing and regeneration and consider possible strategies for its application to improve outcomes in BTE treatments. Technical aspects of exposing cells and tissues to EStim in in vitro and in vivo model systems are also discussed.

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“…It is vital to obtain primary cells or cultured cells with fewer passages owing to the dynamic phenotype of ASCs in vitro. Although the selection of optimal passages has also been studied [87,88], the researchers did not report a consistent conclusion. Future studies need to investigate how to isolate, purify, and characterize ASCs in vitro.…”

Section: Application Of Ascs and Future Perspectivesmentioning

confidence: 99%

“…CD34) decreased [11]. The genetic integrity was preserved with minimal alteration up to five passages [87]. It is vital to obtain primary cells or cultured cells with fewer passages owing to the dynamic phenotype of ASCs in vitro.…”

Section: Application Of Ascs and Future Perspectivesmentioning

confidence: 99%

Adipose-derived stromal cells in regulation of hematopoiesis

et al. 2020

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Over the past decade, mesenchymal stromal cells (MSCs) found in the bone marrow microenvironment have been considered to be important candidates in cellular therapy. However, the application of MSCs in clinical settings is limited by the difficulty and low efficiency associated with the separation of MSCs from the bone marrow. Therefore, distinct sources of MSCs have been extensively explored. Adipose-derived stromal cells (ASCs), a cell line similar to MSCs, have been identified as a promising source. ASCs have become increasingly popular in many fields, as they can be conveniently extracted from fat tissue. This review focuses on the properties of ASCs in hematopoietic regulation and the underlying mechanisms, as well as the current applications and future perspectives in ASC-based therapy.

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Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue

Cited by 41 publications

References 26 publications

Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo

Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines With Vasculogenic Mimicry Ability in vitro and in vivo

Electrical stimulation in bone tissue engineering treatments

Adipose-derived stromal cells in regulation of hematopoiesis

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