Standardization and xanthine oxidase inhibitory potential of Zanthoxylum armatum fruits

Ranjana, .; Nooreen, Zulfa; Bushra, Umme; Jyotshna,; Bawankule, Dnyaneshwar Umrao; Shanker, Karuna; Ahmad, Ateeque; Tandon, Sudeep

doi:10.1016/j.jep.2018.10.018

Cited by 21 publications

(9 citation statements)

References 20 publications

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“…1). A dose dependent Xanthine oxidase inhibitory activity (anti-gout) was observed for the extract in the present study, the standard drug allopurinol showed greater percent of inhibition of Xanthine oxidase than the extract of the compounds with some concentration [33]. IC 50 of xanthine oxidase inhibitory potential of methanolic root extract of Acorus calamus was found to be 310 µg/ml (Graph 2) Gout is a disorder whose prevalence has increased in the last 50 years.…”

Section: Resultssupporting

confidence: 56%

Evaluation of Antioxidant and Xanthine Oxidase Inhibitory Potential of Methanolic Root Extract of Acorus calamus- an In vitro Study

Ahmed

Gayathri

Priya

et al. 2021

JPRI

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Background: Gout is an inflammatory joint disease that elevates the uric acid levels in blood that triggers the formation of urate crystals in the joint,especially since the past 50 years. Xanthine oxidase catalyses oxidative hydroxylation of hypoxanthine to Xanthine to uric acid. show it's higher in men aged above 50 years. Acorus calamus is a mid term, perennial, fragrant herb. Therefore, the main approach for the treatment of gout is reducing uric acid. Aim: To analyze the antioxidant and Xanthine Oxidase inhibitory potential of methanolic root extract of Acorus calamus. Materials and Methods: Preparation of methanolic root extract of Acorus calamus was done by hot percolation method. Phytochemical screening test was done. The antioxidant activity was carried out by DPPH radical scavenging assay. Anti gout potential of the herbal extract was analysed by the evaluation of Xanthine oxidase inhibitory potential. The data were analyzed statistically by a one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test to see the statistical significance among the groups. The results with p<0.05 level were considered to be statistically significant. Results: Methanolic root extract of Acorus calamus shows a strong presence of phytochemicals such as alkaloids ,terpenoids, steroids, saponins, flavonoids.IC50 of antioxidant potential of Methanolic root extract of Acorus calamus was found to be 210 µg/ml.Ic 50 of xanthine oxidase inhibitory potential of methanolic root extract of Acorus calamus was found to be 310 µg/ml. Conclusion: Methanolic root extract of Acorus calamus exhibited significant antioxidant and anti gout potential. Further research on the natural Xanthine oxidase inhibitors especially in vivo studies and investigation of active compounds and its pharmacokinetics to be elucidated.

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Section: Resultssupporting

confidence: 56%

Evaluation of Antioxidant and Xanthine Oxidase Inhibitory Potential of Methanolic Root Extract of Acorus calamus- an In vitro Study

Ahmed

Gayathri

Priya

et al. 2021

JPRI

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“…The crude extract of P. granatum was produced with acetone using water (7 : 3) as the solvent, followed by cleaning with hexane to remove nonpolar compounds. The concentrations and diversity of flavonoids and other phenolic compounds play a critical role in studying the quality and biological potential of plant species [20,21] …”

Section: Resultsmentioning

confidence: 99%

“…The concentrations and diversity of flavonoids and other phenolic compounds play a critical role in studying the quality and biological potential of plant species. [20,21] Despite the structural diversity of flavonoids, the presence of double bonds from the aromatic rings allows these compounds to absorb UV light; thus, the ultraviolet (UV/VIS) spectra are modulated by their chemical structure. Therefore, changes in the substituents result in different profiles, such as bathochromic (by the increase in the number of OH or OCH 3 ) or hypsochromic shifts (by methylation and/or glycosylation).…”

Section: Chemical Analysis Of the Crude Extract And Fractionsmentioning

confidence: 99%

Evaluation of Antioxidant, Antibacterial and Enhancement of Antibiotic Action by Punica granatum Leaves Crude Extract and Enriched Fraction against Multidrug‐Resistant Bacteria

Lima

Silva

Santos

et al. 2021

Chemistry & Biodiversity

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The aim of this study was to evaluate the phytochemical composition, antioxidant, and antimicrobial potential of crude extract and fractions of Punica granatum leaves. The extract was produced by turbo extraction, after which hexanic, ethyl acetate, and aqueous fractions were obtained by partitioning. The chemical analyses were performed by thin layer chromatography and high-performance liquid chromatography, and the antioxidant activities were assayed by DPPH * and ABTS * + . Minimal inhibitory and bactericidal concentrations (MIC/MBC) were applied to twenty-two bacteria. Most strains susceptible to extract/fractions and resistant to antibiotics were selected, and ampicillin, azithromycin, ciprofloxacin, and gentamicin were associated with the ethyl acetate fraction (EAF) against multidrug-resistant strains in modulatory and checkboard models. The data from chromatographic analyses showed flavonoids and tannins in the extract, as well as the enrichment of EAF in phenols, mainly flavonoids. The flavonoids were connected to the electron transfer activity demonstrated in the DPPH * and ABTS * + assays. Gram-positive strains are more susceptible to EAF. The subinhibitory concentrations of P. granatum enhanced the antimicrobial activity of the agents and reduced the EAF individual MIC, and the combination of EAF and antibiotics demonstrated a synergistic effect. These results present a promising approach for developing a therapy in which antioxidant extracts and fractions can be used in combination with antibiotics.

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“…Various polyphenolic compounds extracted from plants, especially flavonoids and procyanidins, have been evaluated previously for their inhibitory capacities against xanthine oxidase (6,(25)(26)(27). Moreover, several systemic in vitro screening studies on xanthine oxidase inhibitory activities of consumed beverages (28,29) and medicinal plants used in traditional medicines around the world have been reported (30)(31)(32)(33)(34)(35).…”

Section: Introductionmentioning

confidence: 99%

Metabolites of Procyanidins From Litchi Chinensis Pericarp With Xanthine Oxidase Inhibitory Effect and Antioxidant Activity

et al. 2021

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Procyanidins from litchi pericarp (LPPC) has been evidenced to possess strong antioxidant activities in vivo that is possibly correlated with their intestinal metabolites. However, the xanthine oxidase inhibitory effect of LPPC and its metabolites was less concerned. In this study, three oligomeric procyanidins and eight metabolic phenolic acids were identified in the urine of rats administrated with LPPC by high performance liquid chromatography and liquid chromatography-mass spectrometry analysis. Data indicated that all the metabolites excreted were significantly increased by the treatment of 300 mg/kg body weight of LPPC (P < 0.05), revealing considerable 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH) and hydroxyl radicals activities of scavenging. Moreover, phenolic metabolites involving epicatechin, A-type dimer, A-type trimer, caffeic acid, and shikimic acid exhibited greater xanthine oxidase inhibition effects compared with other metabolites, with an inhibitory rate higher than 50% at the concentration 200 μg/ml. The IC50 value of these five phenols were 58.43 ± 1.86, 68.37 ± 3.50, 74.87 ± 1.30, 95.67 ± 3.82, and 96.17 ± 1.64 μg/ml, respectively. As a whole, this work suggests that the xanthine oxidase inhibition and antioxidant activity of LPPC-derived metabolites as one of the mechanisms involved in the beneficial effects of LPPC against hyperuricemia or gout.

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Standardization and xanthine oxidase inhibitory potential of Zanthoxylum armatum fruits

Cited by 21 publications

References 20 publications

Evaluation of Antioxidant and Xanthine Oxidase Inhibitory Potential of Methanolic Root Extract of Acorus calamus- an In vitro Study

Evaluation of Antioxidant and Xanthine Oxidase Inhibitory Potential of Methanolic Root Extract of Acorus calamus- an In vitro Study

Evaluation of Antioxidant, Antibacterial and Enhancement of Antibiotic Action by Punica granatum Leaves Crude Extract and Enriched Fraction against Multidrug‐Resistant Bacteria

Metabolites of Procyanidins From Litchi Chinensis Pericarp With Xanthine Oxidase Inhibitory Effect and Antioxidant Activity

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