Standardization of a colorimetric method for the determination of fructose using o-cresol: Sulphuric acid reagent

Kumar, Vishu; Pattabiraman, T. N.

doi:10.1007/bf02867965

Cited by 9 publications

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“…The complete removal of fructose from the fructose imprinted PEG-Fe 3 O 4 nanoparticles was confirmed by o-cresol: H 2 SO 4 assay. 17 The standard range of fructose was between 1 and 300 µmol ml -1 .…”

Section: Elution Of Template (Fructose)mentioning

confidence: 99%

The magnetic fructose imprinted polymer for determination of fructose from apple juice

Okutucu¹

2018

International Journal of Chemistry and Technology

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Molecularly imprinted polymers (MIP) are synthetic receptors that have an ability to recognize and select its template molecule from complex matrix. Nowadays many new approaches of MIPs are researched. One of them is the preparing of magnetic MIPs. Magnetic molecularly imprinted polymer (MMIP) nanoparticles can be a candidate for solid phase extraction adsorbent by the porous morphology, narrow size distribution, stable chemical and thermal property. The aim of this study is to prepare MMIP nanoparticles for solid phase extraction of fructose from apple juice. The PEG treated Fe 3 O 4 magnetic nanoparticles surface was coated with monosaccharide (fructose) imprinted polymer. The monomer acrylamide, cross-linking agent ethylene glycol dimethacrylate (EGDMA), initiator azobisisobutyronitrile (AIBN), the porogen dimethylsulfoxide were used for fructose imprinting. The structural characterization of MMIP was performed by FT-IR, and the imprinting characteristics of polymer were also studied by adsorption experiment and Scatchard analysis. The 65% of fructose was recognized with fructose MMIP from apple juice using only an external permanent magnet without filtration/centrifugation.

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Section: Elution Of Template (Fructose)mentioning

confidence: 99%

The magnetic fructose imprinted polymer for determination of fructose from apple juice

Okutucu¹

2018

International Journal of Chemistry and Technology

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“…The control polymer was also washed with same solutions at the same time. The amount of sucrose extracted from MIP was assayed by o-cresol-sulfuric acid method (Kumar et al 1997).…”

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confidence: 99%

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Telli

Okutucu

2018

KSEJ

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Separation of carbohydrates from different matrices were still remains a challenge because of their wide variety of possible isomers, high polarity and similar chemical structures. There are many chromatographic and colorimetric techniques were usually used for analyzing carbohydrates. Due to their chemical properties that were given above, laborious derivatization procedures were needed for sensitive analysis. In order to overcome these techniques problems, it is necessary to establish a rapid, low cost and efficient method. Molecularly imprinted polymers can be used instead of these conventional techniques because of their easy preparation; no need of sample derivatization and low cost (Okutucu et al. 2009).

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2021

Protocols in Biochemistry and Clinical Biochemistry

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Standardization of a colorimetric method for the determination of fructose using o-cresol: Sulphuric acid reagent

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Cited by 9 publications

References 10 publications

The magnetic fructose imprinted polymer for determination of fructose from apple juice

The magnetic fructose imprinted polymer for determination of fructose from apple juice

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