Standardization of a duplex RT-PCR for the detection of Influenza A and Newcastle disease viruses in migratory birds

Soares, P.; Demétrio, C.; Sanfilippo, Luiz F.; Kawanoto, Adelia Hiroko Nagamori; Brentano, Liana; Durigon, Edison Luíz

doi:10.1016/j.jviromet.2004.09.011

Cited by 27 publications

(19 citation statements)

References 26 publications

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“…NDV, a pathogen of social a n d economical importance, is known t o be disseminated throughout t h e world by migratory and wild birds (Soares et al, 2005). There is also a general concern that NDV wild strains, circulating among wild birds, can become highly pathogenic i n poultry (Shengqing et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Pathogens in Great White Pelicans (Pelecanus onocrotalus) from the Western Cape, South Africa

Assunção

Machado

et al. 2007

Journal of Applied Animal Research

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Section: Resultsmentioning

confidence: 99%

Prevalence of Pathogens in Great White Pelicans (Pelecanus onocrotalus) from the Western Cape, South Africa

Assunção

Machado

et al. 2007

Journal of Applied Animal Research

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“…The negative results found in this study for Influenza viruses are similar to those reported by Buscaglia (2012), who captured 157 birds in Argentina, all of which were tested negative for the presence of the avian influenza virus. On the other hand, Kawamoto et al (2005) and Soares et al (2005) detected the presence of Influenza A viruses in wild and migratory birds in the state of São Paulo. The first subtype H11N9 isolated from migratory birds collected in the Amazon region was recently reported, and was found to present a very high similarity with viral subtypes described in North America (Araujo et al, 2014).…”

Section: Discussionmentioning

confidence: 97%

Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil

Pinto

Ometto

Araújo

et al. 2016

Rev. Bras. Cienc. Avic.

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The Pantanal is the world's largest wetland biome with a seasonal flood pulse that attracts a great diversity of birds, many of which are migratory. Birds can be natural reservoirs Influenza A, West Nile and Newcastle Disease viruses. However, the occurrence of carriers for these viruses in the Pantanal was not verified yet. The present study evaluated the occurrence of natural infection by Influenza A, WN and ND virus of birds in the municipality of Poconé, a subregion of the Pantanal in the state of Mato Grosso, Brazil. A total of 76 birds belonging to 11 orders and 20 families were captured using mist nets. The most representative order was Passeriformes, followed by the other nine orders, which included Columbiformes, Psittaciformes, Charadriiformes and Anseriformes. The most representative family was Thamnophilidae, with 16 individuals (21.0%), followed by the family Tyrannidae with 10 individuals (7.6%) and the family Furnariidae, with eight individuals (10.5%). The bird species were identified, and cloacal and tracheal swab samples were collected. The samples were subjected to RNA extraction and tested for the presence of the three agents by real-time polymerase chain reaction (qRT-PCR). All the sampled birds were considered healthy, had no clinical sign of infection, and were tested negative for the three viruses. Based on our findings, we can conclude that Influenza, West Nile and Newcastle Disease viruses were absent from the samples in this region of the Pantanal wetlands during the period of this study.

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“…The presence of AIV was confirmed by HI testing and real-time reverse transcription polymerase chain reaction (rRT-PCR), and all strains were later characterized as H3N2. 22,23 On the other hand, Soares et al used the same methods but did not detect AIV in 86 ducks (Anatidae) captive at the São Paulo zoo. 22 In 2004, the Brazilian Ministry of Health conducted two surveys for influenza virus in wild birds.…”

Section: Introductionmentioning

confidence: 99%

“…22,23 On the other hand, Soares et al used the same methods but did not detect AIV in 86 ducks (Anatidae) captive at the São Paulo zoo. 22 In 2004, the Brazilian Ministry of Health conducted two surveys for influenza virus in wild birds. The first study examined wild birds of Galinhos, Rio Grande do Norte state.…”

Section: Introductionmentioning

confidence: 99%

Avian Influenza in Wild Birds from South America: Review, Implications and Perspectives

2016

ERHM

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Standardization of a duplex RT-PCR for the detection of Influenza A and Newcastle disease viruses in migratory birds

Cited by 27 publications

References 26 publications

Prevalence of Pathogens in Great White Pelicans (Pelecanus onocrotalus) from the Western Cape, South Africa

Prevalence of Pathogens in Great White Pelicans (Pelecanus onocrotalus) from the Western Cape, South Africa

Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil

Avian Influenza in Wild Birds from South America: Review, Implications and Perspectives

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