Standardization of a new non-invasive device for assessment of arterial stiffness in rats: Correlation with age-related arteries’ structure

Fabrício, Mayara F.; Jordão, Maria Tereza; Miotto, Danyelle Siqueira; Ruiz, Thalles Fernando Rocha; Vicentini, Carlos Alberto; Lacchini, Sílvia; Santos, Carlos; Michelini, Lisete Compagno; Amaral, Sandra Lia do

doi:10.1016/j.mex.2020.100901

Cited by 10 publications

(22 citation statements)

References 19 publications

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“…Assessments of PWV were performed at the beginning and on the 12th day of the experimental protocol, following the procedures already published (Fabricio et al, 2020). In summary, the 2 pOpet® probes (Axelife SAS, Saint Nicolas de Redon, France) were positioned on the right forelimb and hindlimb of the anesthetized rats.…”

Section: Methodsmentioning

confidence: 99%

Differential effects of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats

Tardelli

Duchatsch

Herrera

et al. 2021

J of Applied Toxicology

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Dexamethasone (DEX)‐induced hypertension is observed in normotensive rats, but little is known about the effects of DEX on spontaneously hypertensive animals (SHR). This study aimed to evaluate the effects of DEX on hemodynamics, cardiac hypertrophy and arterial stiffness in normotensive and hypertensive rats. Wistar rats and SHR were treated with DEX (50 μg/kg s.c., 14 d) or saline. Pulse wave velocity (PWV), echocardiographic parameters, blood pressure (BP), autonomic modulation and histological analyses of heart and thoracic aorta were performed. SHR had higher BP compared with Wistar, associated with autonomic unbalance to the heart. Echocardiographic changes in SHR (vs. Wistar) were suggestive of cardiac remodeling: higher relative wall thickness (RWT, +28%) and left ventricle mass index (LVMI, +26%) and lower left ventricle systolic diameter (LVSD, −19%) and LV diastolic diameter (LVDD, −10%), with slightly systolic dysfunction and preserved diastolic dysfunction. Also, SHR had lower myocardial capillary density and similar collagen deposition area. PWV was higher in SHR due to higher aortic collagen deposition. DEX‐treated Wistar rats presented higher BP (~23%) and autonomic unbalance. DEX did not change cardiac structure in Wistar, but PWV (+21%) and aortic collagen deposition area (+21%) were higher compared with control. On the other side, DEX did not change BP or autonomic balance to the heart in SHR, but reduced RWT and LV collagen deposition area (−12% vs. SHRCT). In conclusion, the results suggest a differential effect of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats.

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Section: Methodsmentioning

confidence: 99%

Differential effects of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats

Tardelli

Duchatsch

Herrera

et al. 2021

J of Applied Toxicology

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“…This study confirmed the participation of RAS in hypertension, as shown elsewhere ( Marque et al, 2002 ; Safar, 2010 ; Hong et al, 2013 ; Natalin et al, 2016 ; Mancini et al, 2017 ; Steppan et al, 2020 ), since 8 weeks of perindopril treatment significantly reduced BP (−11%), as shown by the time course of SBP. We did not measure PWV at the beginning of the treatment, but since we have shown that PWV positively correlates with BP in SHR ( Fabricio et al, 2020 ), we may assume that PWV was also high at the beginning of the protocol. Because RAS has also been implicated in vessel remodeling and aortic stiffening, ACE inhibitors play a crucial role in the restoration of the balance between plasma (and tissue) angiotensin II and bradykinin levels ( Ferrari et al, 2005 ), which improves arteriolar structure, independent of their ability to reduce BP ( Mancini et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…After 60 days of pharmacological treatment, the assessment of PWV was performed as previously published ( Fabricio et al, 2020 ). In summary, each rat was anesthetized with xylazine hydrochloride (Anasedan ® , 10 mg/kg) and ketamine hydrochloride (Dopalen ® , 50 mg/kg), and two pOpet ® probes (Axelife SAS, Saint Nicolas de Redon, France) were positioned on the right forelimb (close to elbow) and hindlimb (close to knee).…”

Section: Methodsmentioning

confidence: 99%

“…The possibility of having an experimental model with the measurement of both blood pressure (BP) and PWV, simultaneously with direct access to the arteries for gene, protein, histological studies, and other assays, represents an important advancement for better understanding the mechanisms involved in arterial stiffness changes. In this regard, our group recently standardized a new non-invasive device for assessment of arterial stiffness in rats ( Fabricio et al, 2020 ) and showed that it is able to detect changes in arterial stiffness that are conditioned by age- and pressure-related arterial remodeling.…”

Section: Introductionmentioning

confidence: 99%

“…Aortic stiffening is associated with either a remodeling or disorganization of the vascular wall, which derives from an increased collagenous material, fibrotic components, the presence of elastin fiber fracture, arterial elasticity, or vascular smooth muscle cell (VSMC) hypertrophy ( Morgan et al, 2014 ; Lindesay et al, 2016 , 2018 ; Fabricio et al, 2020 ; Steppan et al, 2020 ). Among several causes of aortic stiffness, the central role of the renin–angiotensin system (RAS) is well known, and therefore, some studies have shown the effects of RAS inhibition on arterial stiffness ( Marque et al, 2002 ; Gonzalez et al, 2018 ) and, consequently, on BP, but the exact molecular mechanisms induced by RAS on aortic stiffness are not completely understood.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach

et al. 2021

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Arterial stiffness, frequently associated with hypertension, is associated with disorganization of the vascular wall and has been recognized as an independent predictor of all-cause mortality. The identification of the molecular mechanisms involved in aortic stiffness would be an emerging target for hypertension therapeutic intervention. This study evaluated the effects of perindopril on pulse wave velocity (PWV) and on the differentially expressed proteins in aorta of spontaneously hypertensive rats (SHR), using a proteomic approach. SHR and Wistar rats were treated with perindopril (SHRP) or water (SHRc and Wistar rats) for 8 weeks. At the end, SHRC presented higher systolic blood pressure (SBP, +70%) and PWV (+31%) compared with Wistar rats. SHRP had higher values of nitrite concentration and lower PWV compared with SHRC. From 21 upregulated proteins in the aortic wall from SHRC, most of them were involved with the actin cytoskeleton organization, like Tropomyosin and Cofilin-1. After perindopril treatment, there was an upregulation of the GDP dissociation inhibitors (GDIs), which normally inhibits the RhoA/Rho-kinase/cofilin-1 pathway and may contribute to decreased arterial stiffening. In conclusion, the results of the present study revealed that treatment with perindopril reduced SBP and PWV in SHR. In addition, the proteomic analysis in aorta suggested, for the first time, that the RhoA/Rho-kinase/Cofilin-1 pathway may be inhibited by perindopril-induced upregulation of GDIs or increases in NO bioavailability in SHR. Therefore, we may propose that activation of GDIs or inhibition of RhoA/Rho-kinase pathway could be a possible strategy to treat arterial stiffness.

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Dexamethasone-Induced Arterial Stiffening Is Attenuated by Training due to a Better Balance Between Aortic Collagen and Elastin Levels

Paula

Tardelli

Amaral

2023

Cardiovasc Drugs Ther

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Standardization of a new non-invasive device for assessment of arterial stiffness in rats: Correlation with age-related arteries’ structure

Cited by 10 publications

References 19 publications

Differential effects of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats

Differential effects of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats

Identification of Aortic Proteins Involved in Arterial Stiffness in Spontaneously Hypertensive Rats Treated With Perindopril:A Proteomic Approach

Dexamethasone-Induced Arterial Stiffening Is Attenuated by Training due to a Better Balance Between Aortic Collagen and Elastin Levels

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