Standardization of corneal alkali burn methodology in rabbits

Villabona-Martínez, Valeria; Sampaio, Lycia Pedral; Shiju, Thomas Michael; Wilson, Steven E.

doi:10.1016/j.exer.2023.109443

Cited by 12 publications

(2 citation statements)

References 14 publications

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“…Importantly, the extent of myofibroblast generation and subsequent fibrosis is directly proportional to the severity of the alkali injury. This was recently demonstrated in rabbit CAB models which were generated using variable NaOH concentrations, whilst controlling its mode of application, duration of exposure and irrigation [97]. Myofibroblasts synthesize copious amounts of fibronectin [98] and collagen types I [29], III [9,41,99] and IV [99].…”

Section: Fibrosismentioning

confidence: 95%

Pathogenesis of Alkali Injury-Induced Limbal Stem Cell Deficiency: A Literature Survey of Animal Models

Sprogyte

Park

Girolamo

2023

Cells

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Limbal stem cell deficiency (LSCD) is a debilitating ocular surface disease that eventuates from a depleted or dysfunctional limbal epithelial stem cell (LESC) pool, resulting in corneal epithelial failure and blindness. The leading cause of LSCD is a chemical burn, with alkali substances being the most common inciting agents. Characteristic features of alkali-induced LSCD include corneal conjunctivalization, inflammation, neovascularization and fibrosis. Over the past decades, animal models of corneal alkali burn and alkali-induced LSCD have been instrumental in improving our understanding of the pathophysiological mechanisms responsible for disease development. Through these paradigms, important insights have been gained with regards to signaling pathways that drive inflammation, neovascularization and fibrosis, including NF-κB, ERK, p38 MAPK, JNK, STAT3, PI3K/AKT, mTOR and WNT/β-catenin cascades. Nonetheless, the molecular and cellular events that underpin re-epithelialization and those that govern long-term epithelial behavior are poorly understood. This review provides an overview of the current mechanistic insights into the pathophysiology of alkali-induced LSCD. Moreover, we highlight limitations regarding existing animal models and knowledge gaps which, if addressed, would facilitate development of more efficacious therapeutic strategies for patients with alkali-induced LSCD.

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Section: Fibrosismentioning

confidence: 95%

Pathogenesis of Alkali Injury-Induced Limbal Stem Cell Deficiency: A Literature Survey of Animal Models

Sprogyte

Park

Girolamo

2023

Cells

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“…A chemical burn was made on one eye of each subject in the chemical burn group using NaOH solution as previously described [13][14][15]. Briefly, a piece of circular filter paper suitable for the size of the cornea was dipped in 0.5 N NaOH solution and then placed on the center of the cornea for approximately 30 s. After washing with a balanced salt solution (Alcon, USA) and levofloxacin (Cravit®, Santen, Osaka, Japan), 0.1% fluorometholone (Flumetholon®, Santen, Japan) and a polymyxin B sulfate-neomycin sulfate-dexamethasone ointment (Forus®, Samil Pharm, Seoul, Republic of Korea) were administered to the eye.…”

Section: Establishment Of the Rabbit Corneal Chemical Burn Modelmentioning

confidence: 99%

Biocompatibility and Transplantation Efficacy of the C-Clear Artificial Cornea in a Rabbit Chemical Burn Model

Chung,

Nam,

Lee

et al. 2023

Bioengineering

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We investigated the bioavailability and stability of a C-Clear artificial cornea in a rabbit chemical burn model. Thirty-six rabbits were divided into a control group (n = 16) and a chemical burn group that used NaOH solution (n = 20). After lamellar dissection, the central posterior lamella was excised using a 3 mm diameter trephine, and an artificial cornea was transplanted into the lamellar pocket. After 2 weeks, the central anterior lamella was excised using a 3 mm diameter trephine to secure a clean visual axis. We examined the anterior segment of the eyes weekly for 12 weeks after transplantation. Successful subjects whose artificial corneas were maintained stably for 12 weeks were euthanized and underwent histologic examinations. Artificial corneas remained stable for up to 12 weeks in 62.5 and 50% of rabbits in the control and chemical burn groups, respectively. Two rabbits in the chemical burn group showed the formation of a retroprosthetic membrane, and one rabbit with visual axis blockage underwent membrane removal using a Nd:YAG laser. In histologic examinations, adhesion between artificial cornea and peripheral corneal stoma was observed. In conclusion, we confirmed structural stability and biocompatibility of the C-Clear artificial cornea for up to 12 weeks after implantation in control and chemical burn groups.

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Animal Models for Limbal Stem Cell Deficiency: A Critical Narrative Literature Review

Atalay,

Altuğ,

Çalışkan

et al. 2024

Ophthalmol Ther

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This literature review will provide a critical narrative overview of the highlights and potential pitfalls of the reported animal models for limbal stem cell deficiency (LSCD) and will identify the neglected aspects of this research area. There exists significant heterogeneity in the literature regarding the methodology used to create the model and the predefined duration after the insult when the model is supposedly fully fit for evaluations and/or for testing various therapeutic interventions. The literature is

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Standardization of corneal alkali burn methodology in rabbits

Cited by 12 publications

References 14 publications

Pathogenesis of Alkali Injury-Induced Limbal Stem Cell Deficiency: A Literature Survey of Animal Models

Pathogenesis of Alkali Injury-Induced Limbal Stem Cell Deficiency: A Literature Survey of Animal Models

Biocompatibility and Transplantation Efficacy of the C-Clear Artificial Cornea in a Rabbit Chemical Burn Model

Animal Models for Limbal Stem Cell Deficiency: A Critical Narrative Literature Review

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