Standardization of DNA extraction and genetic diversity analysis of Haloxylon salicornicum: An underutilized species of extreme arid environment

Genetic diversity and population structure of Haloxylon salicornicum moq. in Kuwait by ISSR markers

“…Haloxylon salicornicum being an cross pollinated [ 50 ] species followed an analogous pattern of genetic differentiation. Our findings were in agreement with related species of Haloxylon distributed in other Asian countries [ 23 , 48 , 49 , 55 ]. The proportion of variation within populations was as high as 89.4% whereas among population was approximately 10% as detected by eight ISSR markers on 195 scorable loci of H .…”

“…To understand the extent of genetic diversity, genetic structure and differentiation among H . salicornicum populations occurring in different geographic regions of Kuwait, the PCR-based ISSR markers, were selected based on their applicability in desert plants that were used for both inter- and intra-population analysis [ 20 – 23 ]. The markers were able to detect 86.5% of polymorphism in the current study.…”

“…ammodendron from China [ 42 ] and 98.5% in H . salicornicum distributed in the Indian Thar desert [ 23 ]. The high rate of polymorphism in Haloxylon communities would be attributed to its demographic features.…”

“…Similar values (79% variation within and 21% among populations) were also obtained through different marker system of RAPD and ISJ which amplified 67 loci in H . salicornicum collected from six locations of Jaisalmer district in India [ 23 ]. The genetic variability of Haloxylon species revealed in this study is important with respect to the ecological conservation and restoration practice.…”

“…The ISSR markers make use of microsatellite sequences that are inherently highly variable and ubiquitously distributed across the genome, at the same time achieving higher reproducibility as compared to other genetic markers [ 18 , 19 ]. ISSR DNA markers have been widely applied in genetic characterization of desert plant species [ 20 – 23 ]. ISSRs have also been used as a technique to establish conservation strategies for endangered plants such as Agave victoriae [ 24 ], Acacia raddiana [ 25 ] and Breonadia salicina [ 26 ].…”