Standardization of in vitro micropropagation of Winter Jasmine (Jasminum nudiflorum) using nodal explants

Bhat, Momin Showkat; Rather, Z.A.; Nazki, Imtiyaz Tahir; Banday, Neelofar; Wani, T. A.; Rafiq, Sadaf; Farooq, Iqra; Noureldeen, Ahmed; Darwish, Hadeer

doi:10.1016/j.sjbs.2022.02.011

Cited by 9 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the unique ability in induction of adventitious roots, auxins are indispensable components of plant tissue culture media in rooting phase. Our findings regarding superior performance of IBA auxin compared to NAA in adventitious root Greenhouse Plant Production Journal 1(1) (2024), 55-62 formation are consistent with earlier reports in other plant species (Cartabia et al, 2022;Farooq et al, 2021;Rafiq et al, 2021;Showkat Bhat et al, 2022). These differences in root induction abilities among auxins can be attributed to factors such as variations in auxin receptors involved in the rooting process and their stability (Mohamad et al, 2022;Cartabia et al, 2022).…”

Section: Discussionsupporting

confidence: 91%

“…Cytokinins and auxins are two major groups of plant growth regulators commonly used in various types of in vitro plant cultures. Similar to results of our study, the principal role of cytokinins in in vitro organogenesis processes including shoot proliferation has been widely reported (Veraplakorn, 2016;Hosseinabadi et al, 2020;Kumar and Giridhar, 2020;Nithya and Kamalam, 2021;Farooq et al, 2022). In fact, cytokinins play a crucial role in stimulating cell division and synthesis of various macromolecules including RNA, proteins and enzymes and they are notably used in in vitro cultures to suppress apical dominance and promote axillary shoot proliferation (Gomes et al, 2010;Mohamad et al., 2022;Farooq et al, 2021;Fawzia et al, 2018;Raju and Divya, 2020).…”

Section: Discussionsupporting

confidence: 88%

“…This method has gained significant commercial importance as it allows for the large-scale propagation of desired plants, thus playing a crucial role in the global ornamental industry. In fact, in vitro cultures offer several advantages over traditional methods of plant propagation, including the relatively rapid mass production of plant species and a reduced cost of production (Nakano et al, 2004;Al-Aizari et al, 2020;Raju and Divya, 2020;Showkat Bhat et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimization of plant growth regulators for in vitro mass propagation of Philodendron cv. Birkin through shoot tip culture

Akramian,

Khaleghi,

Salehi Arjmand

2024

JGPP

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of plant growth regulators for in vitro mass propagation of Philodendron cv. Birkin through shoot tip culture

Akramian,

Khaleghi,

Salehi Arjmand

2024

JGPP

View full text Add to dashboard Cite

“…Direct shoot regeneration from nodal ex plants was reported in many plants like water hyssop (Bacopa monnieri (L.) Pennell) [27], the winter jasmine (Jasminum nudiflorum Lindl.) [28], and Mansonia altissima [29]. Very similar reports, i.e., for cytokinin-induced shoots production, were observed in other plant species like plantain (Musa sp., "Oniaba" and "Apantu pa" cultivars) [30] and medicinal aloe (Aloe vera (L.) Burm.…”

Section: Discussionsupporting

confidence: 76%

In Vitro Propagation and Phytochemical Composition of Centratherum punctatum Cass—A Medicinal Plant

Talan,

Mujib,

Ejaz

et al. 2023

Horticulturae

View full text Add to dashboard Cite

An effective and reproducible micropropagation protocol was developed for Centratherum punctatum Cass. Successful in vitro initiation of callus and subsequent plant regeneration were obtained on nodal explants cultured on MS medium supplemented with plant growth regulators (PGRs). The maximum frequency of callus formation (98.3%) was noted on MS containing 4.0 mg/L 6-Benzylaminopurine (BAP) and 3.5 mg/L Kinetin with a maximum callus weight of 2.02 g. The best shoot induction frequency (100%) with an average of 30.2 shoots per explant was achieved when 4.5 mg/L BAP and 4.0 mg/L Kinetin were added to the MS. The same PGR combination resulted in the best callus-mediated shoot formation (8.3 shoots/callus mass). The highest rhizogenic response (95.3%) with an average 26.1 roots per shoot and root length of 6.2 cm was obtained with 1.0 mg/L Indole-3-acetic acid (IAA)-supplemented MS medium. The gas chromatography–mass spectrometry (GC-MS) technique was applied in the present study to analyze the methanolic extracts of the leaf, callus, and root of regenerated C. punctatum shoots to detect the different phytochemical constituents. The leaf extract of the regenerated C. punctatum showed 37 phytocompounds; some important bioactive compounds were the Phytol,1,6-Octadien 3,5-Dimethyl-Cis, 4,8-Dimethylnona-3,8-dien-2-one, 2,6-Octadiene, Stigmasterol, Chondrillasterol, Lanosteryl acetate, etc. In the callus, the extract had a total of 57 phytocompounds; among them, the Stigmasterol, Guanosine, and Tri-decanoic acid were the major ones. In the root extract, the GC-MS revealed a low number of 23 phytocompounds, the important compounds of which were Stigmasterol, Trimethylsilyl (TMS) derivative, Chrysantenyl 2-methuylbutanoate, 4-tert-Butoxybutan-1-ol, etc. The order in terms of numbers of phytocompounds present in tissue sources are callus > leaf > root.

show abstract

“…Selecting the proper protocol for plant micropropagation is also a crucial factor, which depends on the plant species. For standardization of the micropropagation protocol of any plant species, many studies have been recently reported (e.g., [155][156][157]). Are these challenges facing both developing and developed countries?…”

Section: Discussionmentioning

confidence: 99%

An Academic and Technical Overview on Plant Micropropagation Challenges

et al. 2022

View full text Add to dashboard Cite

The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.

show abstract

Standardization of in vitro micropropagation of Winter Jasmine (Jasminum nudiflorum) using nodal explants

Cited by 9 publications

References 39 publications

Optimization of plant growth regulators for in vitro mass propagation of Philodendron cv. Birkin through shoot tip culture

Optimization of plant growth regulators for in vitro mass propagation of Philodendron cv. Birkin through shoot tip culture

In Vitro Propagation and Phytochemical Composition of Centratherum punctatum Cass—A Medicinal Plant

An Academic and Technical Overview on Plant Micropropagation Challenges

Contact Info

Product

Resources

About