Standardization of in vitro nervous tissue culture for honeybee: A high specificity toxicological approach

Azevedo, Patricia; Butolo, Nicole Pavan; Alencar, Luciano Delmondes de; Soares-Lima, Hellen Maria; Sales, Victor Ribeiro; Malaspina, Osmar; Nocelli, Roberta Cornélio Ferreira

doi:10.1016/j.ecoenv.2019.110040

Cited by 6 publications

(1 citation statement)

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“…[237] Therefore, establishing standardized and effective neural cell culture processes is of great importance and urgency. [238] Given that most of the reported standardized neural culture models focused on animal cells, the development of effective, reproducible protocols for human neural cultures is crucial for engineering human cell-based, innervated MSK tissues. [239] Stem cells are highly sought after in tissue engineering due to their proliferation and differentiation potential.…”

Section: Effective Neuron Culture and Stem Cell Differentiationmentioning

confidence: 99%

Engineering Innervated Musculoskeletal Tissues for Regenerative Orthopedics and Disease Modeling

Zhou,

Liu,

Xiong

et al. 2024

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Musculoskeletal (MSK) disorders significantly burden patients and society, resulting in high healthcare costs and productivity loss. These disorders are the leading cause of physical disability, and their prevalence is expected to increase as sedentary lifestyles become common and the global population of the elderly increases. Proper innervation is critical to maintaining MSK function, and nerve damage or dysfunction underlies various MSK disorders, underscoring the potential of restoring nerve function in MSK disorder treatment. However, most MSK tissue engineering strategies have overlooked the significance of innervation. This review first expounds upon innervation in the MSK system and its importance in maintaining MSK homeostasis and functions. This will be followed by strategies for engineering MSK tissues that induce post‐implantation in situ innervation or are pre‐innervated. Subsequently, research progress in modeling MSK disorders using innervated MSK organoids and organs‐on‐chips (OoCs) is analyzed. Finally, the future development of engineering innervated MSK tissues to treat MSK disorders and recapitulate disease mechanisms is discussed. This review provides valuable insights into the underlying principles, engineering methods, and applications of innervated MSK tissues, paving the way for the development of targeted, efficacious therapies for various MSK conditions.

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Section: Effective Neuron Culture and Stem Cell Differentiationmentioning

confidence: 99%