Standardization of the conditions for extraction of polyphenols from pomegranate peel

Abstract

Pomegranate (Punica granatum) peel possess higher antioxidant activity than the edible portion. Efforts were made to extract dry peel powder at room temperature (28-30°C) using combination of water and ethanol (EtOH, a green combination) and standardize the factors that may affect the extraction efficiency. The storage stability of the extract has been studied for >100 days at room temperature (28-30°C), 4, 0 and −80°C. The extract quality was monitored by measuring the radical scavenging activity (RSA) by diphenyl picrylhydrazyl (DPPH) method, total polyphenol content and by estimating the contents of punicalagins and ellagic acid, the two major ellagitannins present in the peel. The standardized conditions for extraction were found to be; Water and EtOH in 1:1 (v/v) ratio, duration of 24-48 h, ratio of 1:10 for solid to solvent and particle size in the range of 100-400 μ. Consecutive extraction of the peel powder did neither improve the yield nor polyphenol content, hence single extraction was adopted. The extract stored at room temperature for 110 days resulted in 13.2 % loss of polyphenol content followed by 8.9, 2.8, 27.5 and 14.1 % loss in Punicalgin A, B, Ellagic acid and RSA content, respectively.

Processing of pomegranate seed waste and mass transfer studies of extraction of pomegranate seed oil

“…This phenomenon has also been observed in soxhlet extracted (Figure 3g) particles. Similar observation of extraction has also been demonstrated in the peel of pomegranate after polyphenols extraction(Venkataramanamma et al, 2016).…”

Meme kanseri kök hücrelerinde elajik asit ile indüklenmiş miRNA’ların ifadesi ve elajik asidin apoptoz üzerine etkisi

“…EA ve diğer fito-kimyasalları içeren bitki ekstraktları meme kanseri hücrelerinde bölünmeyi durdurmakta ve apoptozu indüklemektedir (10,14). Ekstrakt içeriklerinin HPLC metodu ile incelenmesi, EA'nın diğer moleküllere göre daha fazla bulunduğunu ortaya çıkarmıştır (6,25). EA'nın tek başına meme kanseri hücrelerine uygulandığı çalışmalarda da hücre döngüsünün baskılandığı ve apoptozun meydana geldiği bildirilmiştir (14).…”

Development of ω‐5 and antioxidant enriched bar with pomegranate seed powder

“…Known weight of the control bar and PSP bars were taken and were extracted with 1:1 of Ethanol: water at 27°C, according to the method Venkataramanamma, Aruna, and Singh (2016). The combined extracts were centrifuged, filtered, and stored at room temperature.…”