Standardized methodology for in vitro assessment of bone-to-bone adhesion strength

Bou-Francis, Antony; Ghanem, Amyl

doi:10.1016/j.ijadhadh.2017.03.014

Cited by 17 publications

(4 citation statements)

References 17 publications

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“…Both the in vitro and ex vivo tensile adhesion tests achieved in this study have confirmed that nPDA played such a role in enhancing the bond to bone and titanium of the TTCP/OPS-nPDA glue compared to TTCP/OPS glue in a simulated physiologic environment. Several mechanical testing methods for evaluating the adhesive strength of bone adhesives in vitro/ex vivo have been described [15,16,18,19,[37][38][39][40], showing a wide range of bone bond strength values depending on the type of bone as well as the bone condition and surface preparation. It is worth noting that our experimental conditions were set very "unfavorably" for adhesive bonding, such as a flat surface without any imbrication, the absence of pretreatment, and in a wet environment, all of which were set to simulate the in vivo setting in order to obtain the most reliable data on the adhesive properties.…”

Section: Discussionmentioning

confidence: 99%

“…As well as the major need for the development of adequate bone adhesives, the methods of evaluation and further translation of adhesive candidates into clinical use also face a challenge. Several mechanical testing methods for evaluating the adhesive strength and the compressive resistance of bone adhesives in vitro/ex vivo have been described [15,16,18,19,[37][38][39][40], showing a wide range of bone bond strength values depending on the type of bone along with sample surface preparation and testing conditions. So far, biomimetic CPC/OPS glues have been evaluated in vivo mostly regarding their biocompatibility, osseointegration, or bioresorbability [15,18,20,23,24].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro and In Vivo Evaluation of a Bio-Inspired Adhesive for Bone Fixation

Schlund

Dartus

Defrançois³

et al. 2023

Pharmaceutics

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Compared to metallic hardware, an effective bone adhesive can revolutionize the treatment of clinically challenging situations such as comminuted, articular, and pediatric fractures. The present study aims to develop such a bio-inspired bone adhesive, based upon a modified mineral-organic adhesive with tetracalcium phosphate (TTCP) and phosphoserine (OPS) by incorporating nanoparticles of polydopamine (nPDA). The optimal formulation, which was screened using in vitro instrumental tensile adhesion tests, was found to be 50%molTTCP/50%molOPS-2%wtnPDA with a liquid-to-powder ratio of 0.21 mL/g. This adhesive has a substantially stronger adhesive strength (1.0–1.6 MPa) to bovine cortical bone than the adhesive without nPDA (0.5–0.6 MPa). To simulate a clinical scenario of autograft fixation under low mechanical load, we presented the first in vivo model: a rat fibula glued to the tibia, on which the TTCP/OPS-nPDA adhesive (n = 7) was shown to be effective in stabilizing the graft without displacement (a clinical success rate of 86% and 71% at 5 and 12 weeks, respectively) compared to a sham control (0%). Significant coverage of newly formed bone was particularly observed on the surface of the adhesive, thanks to the osteoinductive property of nPDA. To conclude, the TTCP/OPS-nPDA adhesive fulfilled many clinical requirements for the bone fixation, and potentially could be functionalized via nPDA to offer more biological activities, e.g., anti-infection after antibiotic loading.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Evaluation of a Bio-Inspired Adhesive for Bone Fixation

Schlund

Dartus

Defrançois³

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…Adhesion strength of the materials was tested using a previously established protocol, where investigated biomaterials were melted and applied to two bone sections before curing. The mechanical properties of the set fixation was then tested through compressive and tensile force [ 71 ]. The blends retain the adhesive strength of PCL whist having improved hydrophilicity.…”

Section: Composite Materialsmentioning

confidence: 99%

Strategies for Enhancing Polyester-Based Materials for Bone Fixation Applications

et al. 2021

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Polyester-based materials are established options, regarding the manufacturing of bone fixation devices and devices in routine clinical use. This paper reviews the approaches researchers have taken to develop these materials to improve their mechanical and biological performances. Polymer blending, copolymerisation, and the use of particulates and fibre bioceramic materials to make composite materials and surface modifications have all been studied. Polymer blending, copolymerisation, and particulate composite approaches have been adopted commercially, with the primary focus on influencing the in vivo degradation rate. There are emerging opportunities in novel polymer blends and nanoscale particulate systems, to tune bulk properties, and, in terms of surface functionalisation, to optimise the initial interaction of devices with the implanted environment, offering the potential to improve the clinical performances of fracture fixation devices.

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“…As well as the major need for the development of adequate bone adhesives, the methods of evaluation and further translation of adhesive candidates into clinical use also face a challenge. Several mechanical testing methods for evaluating the adhesive strength and the compressive resistance of bone adhesives in vitro/ ex vivo have been described (15,16,18,19,(37)(38)(39)(40), showing a wide range of bone bond strength values depending on the type of bone as well as bone condition and surface preparation. So far, biomimetic CPC/OPS glues have been evaluated in vivo mostly regarding their biocompatibility, osseointegration or bioresorbability (15,18,20,23,24).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo evaluation of a bio-inspired adhesive for bone fixation

Schlund

Dartus

Defrançois

et al. 2023

Preprint

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Background: Compared to metallic hardware, an effective bone adhesive can revolutionize the treatment of clinically challenging situations such as comminuted, articular and pediatric fractures. The present study aims at developing such a bio-inspired bone adhesive, based upon a modified mineral-organic adhesive with tetracalcium phosphate (TTCP) and phosphoserine (OPS) by incorporating nanoparticles of polydopamine (nPDA). Methods: The optimal formulation was screened using in vitro instrumental traction tests. Adhesion to bone, cohesion, setting time, and biomineralization of the optimized adhesive was then assessed with ex vivo instrumental and manual tests, compression tests, setting time measurement and simulated body fluid assay. Cytotoxicity was assessed by extraction test (ISO 10993-5). A new in vivo model was developed: the rat fibula was glued to the ipsilateral tibia, simulating the clinical scenario of autograft fixation under low mechanical load. Analysis was performed clinically, radiologically with micro-computed tomography and histologically (without decalcification). Results: The optimal formulation was found as 50%molTTCP/50%molOPS-2%wtnPDA with a liquid-to-powder ratio of 0.21 mL/g. This adhesive has a substantially stronger adhesive strength (1.0–1.6 MPa) to bovine cortical bone (after a 24-hour soak in a physiological saline) than the adhesive without nPDA (0.5–0.6 MPa), and is more quickly induced and has more abundant surface mineralization when immersed in simulated body fluid. Moreover, non-cytotoxicity of this adhesive was confirmed in vitro. In vivo, the TTCP/OPS-nPDA adhesive (n=7) was shown to be effective in stabilizing the graft without displacement (clinical success rate of 86% and 71% respectively at 5 and 12 weeks) compared to a sham control (0%). Significant coverage of newly formed bone was particularly observed on the surface of the adhesive, thanks to the osteoinductive property of nPDA. Conclusions: To conclude, the TTCP/OPS-nPDA adhesive fulfilled many clinical requirements for the bone fixation, and potentially could be functionalized via nPDA to offer more biological activities, e.g. anti-infection after antibiotics loading. Trial Registration: Not applicable

show abstract

Standardized methodology for in vitro assessment of bone-to-bone adhesion strength

Cited by 17 publications

References 17 publications

In Vitro and In Vivo Evaluation of a Bio-Inspired Adhesive for Bone Fixation

In Vitro and In Vivo Evaluation of a Bio-Inspired Adhesive for Bone Fixation

Strategies for Enhancing Polyester-Based Materials for Bone Fixation Applications

In vitro and in vivo evaluation of a bio-inspired adhesive for bone fixation

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