Standardized Tape Stripping: A Practical and Reproducible Protocol to Reduce Uniformly the Stratum Corneum

Goulioumis, Alexandros; Gambichler, Thilo; Fluhr, Joachim W.; Kamphowe, Jeanette; Altmeyer, Peter; Kuß, Oliver

doi:10.1007/978-3-319-32383-1_28

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…Hence, the skin is not manipulated prior to the experiment, that is, cut, squeezed, or stretched, but remains in its original state throughout the whole experiment. The ex vivo pig ear model is typically used for the classical tape stripping procedure [31,[41][42][43][44], but can also be used to evaluate the penetration depth into the stratum corneum by epifluorescence microscopy if the drug substance is fluorescent [26]. For this, punch biopsies of the treated skin areas are taken, cut into thin slices, and analysed via confocal laser scanning microscopy or simply by fluorescence microscopy (Fig.…”

Section: Theoretical Aspects For the Development Of The Skin Penetration Modelmentioning

confidence: 99%

Dermal Penetration Analysis of Curcumin in an ex vivo Porcine Ear Model Using Epifluorescence Microscopy and Digital Image Processing

Pelikh

Pinnapireddy

Keck

2021

Skin Pharmacol Physiol

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Introduction: Curcumin is a promising drug candidate, but its use for dermal application is limited due to its poor aqueous solubility. Thus, formulations that increase the solubility of curcumin are needed to fully exploit the therapeutic potential of curcumin. Various previous studies address this issue, but a comparison of the efficacy between these formulations remains difficult. The reason for this is a missing standard formulation as benchmark control and an easy-to-use skin penetration model that allows for a fast discrimination between different formulations. Objective: Thus, the aims of this study were the development of a curcumin standard formulation and a screening tool that allows for a fast discrimination between the dermal penetration efficacies of curcumin from different formulations. Methods: Ethanolic curcumin solutions were selected as simple and easy to produce standard formulations, and the ex vivo porcine ear model, coupled with epifluorescence microscopy and subsequent digital image analysis, was utilized to determine the dermal penetration efficacy of curcumin from the different formulations. Results: Results show that the utilized skin penetration model is a suitable and versatile tool that enables not only a fast determination of the dermal penetration efficacy of curcumin from different formulations but also a detailed and mechanistic information on the fate of chemical compounds after dermal penetration. Ethanolic solutions containing 0.25% curcumin were found to be the most suitable standard formulation. Conclusions: Results of the study provide a new, effective screening tool for the development of dermal formulations for improved dermal delivery of curcumin.

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Section: Theoretical Aspects For the Development Of The Skin Penetration Modelmentioning

confidence: 99%

Dermal Penetration Analysis of Curcumin in an ex vivo Porcine Ear Model Using Epifluorescence Microscopy and Digital Image Processing

Pelikh

Pinnapireddy

Keck

2021

Skin Pharmacol Physiol

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“…One method for delivering allergens to dermal Langerhans cells is by disruption of the stratum corneum with adhesive strips, which also stimulates keratinocytes, enhances Th1 cytokine production, and further activates Langerhans cells [70,71]. Another method for EPIT is by applying an occlusive, polyethylene membrane (Viaskin EDS), allowing for the diffusion of allergens through perspiration without disruption of the stratum corneum [72].…”

Section: Epicutaneous Immunotherapymentioning

confidence: 99%