Standards for photoluminescence quantum yield measurements in solution (IUPAC Technical Report)

Abstract

The use of standards for the measurement of photoluminescence quantum yields (QYs) in dilute solutions is reviewed. Only three standards can be considered well established. Another group of six standards has been investigated by several independent researchers. A large group of standards is frequently used in recent literature, but the validity of these is less certain. The needs for future development comprise: (i) confirmation of the validity of the QY values of many commonly used standard materials, preferably in the form of SI traceable standards; (ii) extension of the set of standard materials to the UV and near-IR spectral ranges; and (iii) good standards or robust protocols for the measurements of low QYs.

The photophysics of LOV-based fluorescent proteins — new tools for cell biology

“…However, the comparison of the fluorescence spectra of a fluorescent dye with known Φ F (standard) and that of the fluorophore under investigation is error prone, since it requires proper correction for differences in absorption and fluorescence spectra. The instrumental setup with the integrating sphere that has been used here was first verified by measuring the quantum yield (ΦF) of the well characterized fluorescent dye fluorescein in 0.1M NaOH(aq) (published ΦF between 0.91 and 0.95 55 ; measured ΦF = 0.931 ± 0.008), as well as riboflavin (published Φ F = 0.26 56 ; measured Φ F = 0.247 ± 0.007) and flavin adenine dinucleotide (published Φ F = 0.03 57 ; measured Φ F = 0.032 ± 0.0004). All three determined fluorescence quantum yields (low, intermediate and high) are in excellent agreement with the literature data and prove the high accuracy of measurements with an integrating sphere.…”

Symmetrically Disubstituted Bithiophene Derivatives of 1,3,4-Oxadiazole, 1,3,4-Thiadiazole, and 1,2,4-Triazole – Spectroscopic, Electrochemical, and Spectroelectrochemical Properties

“…29 Spectroelectrochemical investigations were carried out using Ocean Optics QE65000 and NIRQuest512 diode array spectrometers. In these experiments the electropolymerization products were deposited on an ITO transparent electrode in the same conditions as in the case of the electropolymerization on platinum (vide supra).…”

Hydrothermal Synthesis of Photoluminescent Nanocarbon from Hydroxylic Acids and Amines

“…The quantum yield of the CDs was determined by the comparative method [19]. The quinine sulfate solution in 0.5 M H 2 SO 4 was used as the reference [20]. A drop of aqueous solution of the CDs was dried on a TEM grid which was pretreated by plasma in vacuum to make the surface hydrophilic.…”