ObjectiveTo report the technique, surgical approach, and postoperative features in horses treated via a 3‐dimensional (3D) printed guide‐assisted keratoma resection created using computed tomography (CT) or magnetic resonance imaging (MRI)‐based segmentation.AnimalsFive client‐owned horses.Study designShort case series.MethodsHorses were placed under general anesthesia for imaging (CT and MRI) and underwent a second anesthesia for surgery. Two horses had guides created from CT‐based imaging, 3 horses had guides created from MRI. Various sized nonarbored hole saws were used to create accurate and precise portals for keratoma removal. Surgical sites were managed until keratinized granulation tissue had formed and the defect was sealed with an artificial hoof wall patch.ResultsAll keratomas were successfully removed as a single piece either intact with the hoof wall or easily extracted after the hoof wall portal was created in a surgical time between 20 and 90 min. All CT created guides fitted without issue; MRI‐created guides required minor adjustments with a rotary device for proper fit. All cases had minor debridement adjacent to P3 and circumferential lamellar tissue. All horses returned to previous level of performance 2 to 4 months postoperatively.ConclusionUse of 3D printed guides led to accurate targeting of keratomas with small surgical portals and short surgical times. Due to challenges with MRI‐based segmentation, CT is preferred.