Study design: Single case study. Objectives: To evaluate the energy expenditure during ambulation with the Advanced Reciprocating Gait Orthosis (ARGO), with and without functional electrical stimulation (FES), and with the Parastep system in a single subject, in order to avoid the e ect of intersubject variability. Setting: The Centre of Sport Medicine and Bioengineering Centre`Don C Gnocchi' Foundation ONLUS IRCCS, Milano, Italy. Methods: A single patient (lesion level T 5 ± T 6 ) was trained speci®cally for each walking system and was evaluated after each training period. The e ects of FES on muscle conditioning, spasticity and bone density were also evaluated. The HR/ VO 2 relationship and the energy cost of locomotion were measured during wheelchair (WHCH) use, during locomotion with ARGO (with and without FES) and Parastep system at di erent speeds. Results: The following was observed at the end of the whole training: (a) circumferences of both lower limbs and quadriceps forces were increased, whereas fatigue index was slightly decreased, spasticity and bone density were unchanged; (b) compared to WHCH locomotion, the slope of HR/ VO 2 curves with ARGO was higher (slope di erence=51.1 b 1O 2 71 ), with ARGO+FES was similar (slope di erence=75.3 b 1O 2 71 ) and with Parastep was smaller (slope di erence=755.6 b 1O 2 71 ); (c) HR increased linearly with all locomotion systems, but did not rise above 125 bpm with Parastep; (d) the cost of locomotion was higher with Parastep than with ARGO (with and without FES), tested at each velocity; (e) Parastep appears to be easier to use for the subject. Conclusions: (a) FES can improve ambulation with orthosis, but the cost of locomotion remains very high; (b) the Parastep assisted gait elicits a higher energy expenditure than other orthoses, probably due to the lower speed of locomotion and to the high isometric e ort of the stimulated muscles. Sponsorship: This work has been partially supported by the Italian Minister of Public Health (Ricerca Finalizzata IRCCS no ICS030.7/RF97.25).