The article focuses on startups within creative industries and the high-tech sector (the so-called TIME sector) and the relevance of the factors affecting the location choice of startups within these newly emerging industries. In terms of building a prospering startup ecosystem, results of this empirical research provide recommendations for representatives and designers in charge of destination management and regional development. For founders and entrepreneurs, the research will define which factors are relevant for their location choice. The article argues that the perspectives of stakeholders (entrepreneurs/startups and external experts) differ in some ways on the relevance of certain location factors. It also argues that the main challenge for destination management and regional development will be the dilemma between a generally positive economic situation and the development of a prosperous startup ecosystem.