Stanniocalcin kinetics in freshwater and seawater european eel (Anguilla anguilla)

Hanssen, Rob; Mayer-Gostan, N.; Flik, G.; Bonga, S.E. Wendelaar

doi:10.1007/bf00004604

Cited by 15 publications

(3 citation statements)

References 27 publications

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“…Also, the metabolic clearance, secretion rate, and distribution space for STC are drastically different in freshwater and seawater eel (15). Therefore, we hypothesize that fish adapted to seawater and freshwater provide powerful models with which to study changes in PTHrP and STC activity and their influence on Mrp2-mediated transport, Mrp2 function, and Mrp2 expression.…”

Section: Discussionmentioning

confidence: 99%

Endothelin and calciotropic hormones share regulatory pathways in multidrug resistance protein 2-mediated transport

Wever¹,

Masereeuw²,

Miller³

et al. 2007

American Journal of Physiology-Renal Physiology

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Wever KE, Masereeuw R, Miller DS, Hang XM, Flik G. Endothelin and calciotropic hormones share regulatory pathways in multidrug resistance protein 2-mediated transport. Am J Physiol Renal Physiol 292: F38 -F46, 2007. First published August 15, 2006; doi:10.1152/ajprenal.00479.2005.-The kidney of vertebrates plays a key role in excretion of endogenous waste products and xenobiotics. Active secretion in the proximal nephron is at the basis of this excretion, mediated by carrier proteins including multidrug resistance protein 2 (Mrp2). We previously showed that Mrp2 function is reduced by endothelin-1 (ET-1) through a basolateral B-type receptor, nitric oxide (NO), cGMP, and PKC (Notenboom S, Miller DS, Smits P, Russel FGM, Masereeuw R. Am J Physiol Renal Physiol 282: F458 -F464, 2002; Notenboom S, Miller DS, Smits P, Russel FG, Masereeuw R. Am J Physiol Renal Physiol 287: F33-F38, 2004). This pathway was rapidly activated by several nephrotoxicants and appeared to be calcium dependent. In the present study, we studied the effect of the calciotropic hormones parathyroid hormone (PTH), PTH-related protein (PTHrP), and stanniocalcin (STC) to interfere with ET-regulated Mrp2 transport. Like ET-1, PTH reduces Mrp2-mediated transport by 40% in killifish renal proximal tubules. When given in combination, an additive effect was seen, which is partially reversed by the PKC inhibitor calphostin C. Recombinant PTHrP shows a comparable inhibitory effect, which is concentration dependent and additive to the inhibition by ET. STC fully reverses PTHrPinhibited transport as does a guanylyl cyclase inhibitor. Finally, to confirm PTHrP bioactivity in a homologous assay, we performed immunolocalization and transport studies in sea bream kidney tubules. Mrp2 immunoreactivity was observed in ϳ40% of the tubules and is associated with the brush-border and apical plasma membrane of cells. Both proximal tubules and distal (collecting) tubules express the antigen. A highly significant 40% inhibition of Mrp2-mediated transport was observed with PTHrP in sea bream tubules. In conclusion, ET-regulated Mrp2 transport is influenced by calciotropic hormones and involves PKC and cGMP signaling. parathyroid hormone; parathyroid hormone-related protein; stanniocalcin; xenobiotic transport; fluorescein-methotrexate; Sparus auratus; gilthead sea bream; Fundulus heteroclitus; killifish THE KIDNEY OF VERTEBRATES plays a key role in excretion of endogenous waste products and xenobiotics. Active secretion in the proximal nephron is at the basis of this excretion. Secretion of xenobiotics in proximal tubules is mediated by carrier proteins, including members of the ATP-binding cassette (ABC) superfamily of transmembrane proteins (53). The multidrug resistance-associated protein 2 (Mrp2; ABCC2) belongs to this family. This transporter is found in liver canalicular membranes (6, 37), luminal membranes of the intestine (52), and luminal membranes of renal proximal tubule cells (42). Mrp2 transports a plethora of chemicals, from large lipophilic, organic ani...

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Section: Discussionmentioning

confidence: 99%

Endothelin and calciotropic hormones share regulatory pathways in multidrug resistance protein 2-mediated transport

Wever¹,

Masereeuw²,

Miller³

et al. 2007

American Journal of Physiology-Renal Physiology

View full text Add to dashboard Cite

show abstract

“…It was concluded that cortisol clearance rates were higher in fully SW-adapted fish than in fully FW-adapted fish (Henderson et al 1974;Leloup-Hatey 1974). Most likely, the distribution space of the hormone is higher in SW than in FW, possibly because an in crease in perfusion of organs such as the gills (Hanssen et al 1993). Accordingly, Redding et al (1984) have demonstrated that gills of SW-adapted coho salmon take up and retain more cortisol than tissue from FW controls.…”

Section: Discussionmentioning

confidence: 99%

Regulation of interrenal function in freshwater and sea water adapted tilapia (Oreochromis mossambicus)

Balm

Haenen

Bonga

1995

Fish Physiol Biochem

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In teleosts, cortisol is one of the key factors regulating the adaptation to environmental challenges, such as salinity changes. This paper compares interrenal function between fully adapted freshwater (FW) and sea water (SW) specimens of the euryhaline teleost Oreochromis mossambicus (tilapia), combining morphometric and biosynthetic approaches. Interrenal tissue and two tissues producing interrenal secretagogues (ACTH and A N P; atrial natriuretic peptide) were studied. The results demonstrate that sea water adaptation concurs with a sustained stimulation of the interrenal cells, as evidenced by a marked hyperplasia of the cells and the higher initial ex vivo cortisol release in seawater adapted tilapia. This difference was not reflected in ultrastructural differences in the pituitary corticotropes. Plasma ACTH levels were also similar in FW and SW adapted tilapia. Moreover, in vitro data indicate that the A C T H sensitivity of the interrenal cells of both groups was also similar. A second potential interrenal secretagogue (ANP) has recently been implicated in teleost ionic regulation during salinity changes. However, plasma immunoreactive A N P levels and in vitro production of the hormone were also indistinguishable between FW and SW tilapia. A N P pretreatment of tilapia head kid neys in vitro strongly inhibited the response to A C T H , an effect previously undocumented for teleosts.Whereas the sustained stimulation of the interrenal cells under sea water conditions corroborates results obtained with other teleost species, thereby supporting a hypoosmoregulatory role for cortisol, it also is evident that notable species differences exist regarding the regulation of the interrenal gland under these conditions.

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“…Another conclusion that can be drawn from these findings is that despite the fact that marine fish synthesize and secrete greater amounts of STC (12,14,39,40), this does not require an increase in the Ca 2ϩ sensitivity of the cells. The most likely explanations for this are the moderate hypercalcemia and CS gland hyperplasia that accompany seawater-adaptation, which together would raise the basal rate of secretion without any change in the Ca 2ϩ sensitivity of the cells.…”

Section: R1181mentioning

confidence: 84%

Stanniocalcin in the seawater salmon: structure, function, and regulation

Wagner

Jaworski

Haddad

1998

American Journal of Physiology-Regulatory, Integrative and Comp

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Stanniocalcin (STC) is a homodimeric glycoprotein hormone that was first discovered in fish, where it is produced by unique endocrine glands known as the corpuscles of Stannius (CS). In freshwater salmon, STC plays an integral role in Ca2+ and phosphate homeostasis. High levels of extracellular Ca2+promote the synthesis and release of STC, which on entering the bloodstream reduces the levels of gill and gut Ca2+ transport and renal phosphate excretion to restore normocalcemia. In this report, we have examined STC in seawater salmon. We have studied the distribution of STC protein and mRNA in marine Atlantic salmon CS cells, the responsiveness of these cells to Ca2+, and some physical properties of the hormone. Our results demonstrated that all Atlantic salmon CS cells expressed the STC gene. Furthermore, these cells exhibited a Ca2+ sensitivity that was remarkably similar to those in freshwater salmon in terms of its ability to stimulate STC secretion and gene expression. When Atlantic salmon glands were fractionated by concanavalin A (ConA)-Sepharose chromatography, two distinct forms of the hormone were identified, both of which were recognized by sockeye salmon STC antiserum, and designated as STC1 and STC2. STC1 was a glycosylated, 42-kDa disulfide-linked dimer, with a high affinity for ConA. STC2 did not bind to ConA, was 44 kDa in size, and had a different subunit structure. STC2 was also a less effective inhibitor of gill Ca2+ transport in fish. Collectively, the results suggest that there is a second form of STC in salmon.

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Stanniocalcin kinetics in freshwater and seawater european eel (Anguilla anguilla)

Cited by 15 publications

References 27 publications

Endothelin and calciotropic hormones share regulatory pathways in multidrug resistance protein 2-mediated transport

Endothelin and calciotropic hormones share regulatory pathways in multidrug resistance protein 2-mediated transport

Regulation of interrenal function in freshwater and sea water adapted tilapia (Oreochromis mossambicus)

Stanniocalcin in the seawater salmon: structure, function, and regulation

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