Staphage lysate: an immunomodulator of the primary immune response in mice

Esber, Henry J.; Ganfield, David; Rosenkrantz, Harris

doi:10.1016/0162-3109(85)90032-3

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…Western blot and PMPT of pooled sera indicate that antibody (IgG) to identifiable protein in phage lysate were associated with protection. Esber et al (1985) revealed that staphylococcal phage lysate elicit a significant increase in antistaphylococcal IgG1, IgG2a and IgG2b level in experimented animals. Many attempts have been tried to explore specific immunogenic proteins of P. multocida isolates from HS and FC.…”

Section: Resultsmentioning

confidence: 93%

Active Immunization with Pasteurella multocida Lysate Elicits Antibody that Protects Rabbits against Virulent Pasteurella multocida and Protects Mice by Passive Immunization

Durairajan

Verma

Prajapati

et al. 2021

IJAR

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Background: Pasteurellosis is an economically important disease of livestock worldwide and vaccination is effective mean to control the disease outbreaks. One of alternative method for vaccine preparation could be the use of lytic phages as bacterial inactivating agents and employing “phage lysate immunogen” as an immunizing agent. Therefore, the study was undertaken to explore the protective immune response of novel phage lysate vaccine against Pasteurella multocida. Methods: A phage lysate vaccine of Pasteurella multocida was prepared using isolated lytic phage and subjected to vaccine response study in rabbits. In this experiment, two mouse fixed-dose of lysate was used and the prophylactic efficacies of lysates were evaluated in rabbits by passive mouse protection test (PMPT), western blotting and direct virulent challenge.Result: Passive transfer of antibodies to mice using post-immunization sera of rabbits protected the animals against the challenge with A: 1 strain (75%) and B: 2 strain (50%). In western blotting, a total number of 4 bands were observed in the region between 130 kDa to 25 kDa in the protein of the phage lysate. The study suggested the significance increase in humoral immunity by phage lysate in murine Pasteurellosis.

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Section: Resultsmentioning

confidence: 93%

Active Immunization with Pasteurella multocida Lysate Elicits Antibody that Protects Rabbits against Virulent Pasteurella multocida and Protects Mice by Passive Immunization

Durairajan

Verma

Prajapati

et al. 2021

IJAR

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“…SPL was shown to increase the production of immunoglobulins in human lymphocyte culture and to induce the production of polyclonal immunoglobulins by murine splenocytes (Sulakvelidze and Kutt er, 2005). One dose of SPL administered to mice induced the production of IgG and, to a lesser extent, IgA (Esber et al, 1985). According to Esber et al (1985), SPL can also stimulate a specifi c humoral response in mice.…”

Section: Immunomodulatory Activity Of Staphage Lysatementioning

confidence: 99%

“…One dose of SPL administered to mice induced the production of IgG and, to a lesser extent, IgA (Esber et al, 1985). According to Esber et al (1985), SPL can also stimulate a specifi c humoral response in mice. Thus, an additional benefi cial eff ect of SPL in the treatment of chronic staphylococcal infections may be the enhancement of antibacterial immune responses.…”

Section: Immunomodulatory Activity Of Staphage Lysatementioning

confidence: 99%

“…Thus, an additional benefi cial eff ect of SPL in the treatment of chronic staphylococcal infections may be the enhancement of antibacterial immune responses. Furthermore, SPL, as a preparation stimulating both humoral and cellular immunity, could be particularly useful in the treatment of infections in immunocompromised patients, including those receiving cytostatic drugs and individuals with immunodefi ciencies (Esber et al, 1985). The immunostimulatory eff ects of phage preparations are probably mediated by both phage virions and some components of bacterial cells present in preparations (Sulakvelidze and Kutt er, 2005).…”

Section: Immunomodulatory Activity Of Staphage Lysatementioning

confidence: 99%

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Bacteriophages in health and disease

Hyman¹,

Abedon²

2012

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Through the application of molecular and cellular microbiology we now recognise the diversity and dominance of microbial life forms on our planet, that exist in all environments. These microbes have many important planetary roles, but for we humans a major problem is their ability to colonise our tissues and cause disease. The same techniques of molecular and cellular microbiology have been applied to the problems of human and animal infection during the past two decades and have proved to be immensely powerful tools in elucidating how microorganisms cause human pathology. This series has the aim of providing information on the advances that have been made in the application of molecular and cellular microbiology to specifi c organisms and the diseases that they cause. The series is edited by researchers active in the application of molecular and cellular microbiology to human disease states. Each volume focuses on a particular aspect of infectious disease and will enable graduate students and researchers to keep up with the rapidly diversifying literature in current microbiological research.

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“…The difference between the mean serum IgG concentration of lysate vaccinated and conventional vaccinated mice at 120, 150 and 180 DPI was found to be significant (P<0.01) with higher IgG concentration in lysate vaccinated mice than the conventional vaccinated mice. Esber et al (1985) reported that staphylococcal phage lysate (SPL) administration caused a significant increase in anti-staphylococcal IgG1, IgG2a and IgG2b level in treated animals.…”

Section: Serum Igg Levels In Vaccinated Micementioning

confidence: 99%

Some immunological studies on a new vaccine derived frombacteriophage lysate of Pasteurella multocida in mice

Saxena

Pushpa

Qureshi

2017

IJAR

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The study was undertaken to evaluate the humoral immune response in mice induced by a novel phage lysate vaccine against Hemorrhagic Septicemia developed from Pasteurella multocida grown under iron-restricted conditions and lysed by a bacteriophage. Two groups of mice were immunized with lysate vaccine (LV) and conventional killed HS vaccine (CV) and blood was collected at various days post -immunization (DPI). The serum protein concentration in lysate vaccinated mice (4.675±0.223) was significantly higher (p<0.05) than the conventional vaccinated mice (4.100±0.282) at 150 DPI. The serum globulin levels at 90 DPI and 180 DPI in LV mice (1.330±0.071 and 0.650±0.100) were significantly (p<0.01) higher than the CV mice (0.850±0.084 and 0.366±0.098). The serum IgG levels at 150 DPI and 180 DPI in LV (0.564±0.188 and 0.485±0121) mice were significantly higher (p<0.01) than the CV mice (0.178±0.039 and 0.121±0.026). Although our results in mice are promising, further studies involving bovines are needed.

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Staphage lysate: an immunomodulator of the primary immune response in mice

Cited by 6 publications

References 13 publications

Active Immunization with Pasteurella multocida Lysate Elicits Antibody that Protects Rabbits against Virulent Pasteurella multocida and Protects Mice by Passive Immunization

Active Immunization with Pasteurella multocida Lysate Elicits Antibody that Protects Rabbits against Virulent Pasteurella multocida and Protects Mice by Passive Immunization

Bacteriophages in health and disease

Some immunological studies on a new vaccine derived frombacteriophage lysate of Pasteurella multocida in mice

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