Staphylococcal biofilms impair wound healing by delaying reepithelialization in a murine cutaneous wound model

Abstract

Bacterial biofilms have gained increasing visibility in recent years as a ubiquitous form of survival for microorganisms in myriad environments. A number of in vivo models exist for the study of biofilms in the setting of medically relevant implanted foreign bodies. Growing evidence has demonstrated the presence of bacterial biofilms in the setting of a number of chronic wound states including pressure sores, diabetic foot ulcers, and venous stasis ulcers. Here we present a novel murine cutaneous wound system that directly demonstrates delayed reepithelialization caused by the presence of a bacterial biofilm. We established biofilms using either Staphylococcus aureus or Staphylococcus epidermidis in splinted cutaneous punch wounds created on the backs of normal C57Bl6/J mice. Wound reepithelialization was significantly delayed by bacterial biofilms. This effect was specifically dependent on the ability of the bacteria to form biofilms as demonstrated by exogenous administration of biofilm inhibiting peptides and the use of mutant Staphylococcus spp. deficient in biofilm formation. This represents the first direct evidence for the effect of bacterial biofilms on cutaneous wound healing.

Perspectives on the prevention and treatment of infection for orthopedic tissue engineering applications

“…Infection is known to inhibit healing in several tissue types, including bone, gastrointestinal epithelium, and cutaneous epithelium [8][9][10]. Physiologic wound healing in response to injury occurs in four sequential and timed phases: hemostasis, inflammation, proliferation and repair, and remodeling.…”

Efficcacy of Lantana camara Linn. leaf extracts ointment on dermal wound healing were infected with Staphylococcus epidermidis

“…Therefore, biofilm formed by bacteria inhibit on dermal wound healing. 36 Based on the study result, it turn out that local infection inhibits on dermal wound healing. This happens can caused by host immune response and synergistic interactions among bacteria species that infect.…”

d-amino Acid Inhibits Biofilm but not New Bone Formation in an Ovine Model

“…For orthopaedic infections, Staphylococcus aureus is the most commonly isolated agent, accounting for more than 1 . 2 of all infections [28]. In addition to increasing trends of antimicrobial resistance, the ability of bacteria to develop and persist in biofilms on implanted biomedical devices is recognized as a major factor contributing to chronic relapsing infections and nonosseous union [4,35,42,44,48,49].…”