Staphylococcal Infections

Ruben, Frederick L.; Norden, Carl W.

doi:10.1007/978-1-4757-1140-0_28

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1993

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“…We were interested in studying the antibiotic resistance plasmids in Staphylococcus aureus, the cause of more than 10% of all nosocomial infections [1]. It has been found that more than 90% of clinical isolates of S. aureus obtained from Calcutta hospitals are resistant to the penicillin group of antibiotics [2].…”

Section: Introductionmentioning

confidence: 99%

Transformation and expression of a staphylococcal plasmid in Escherichia coli

Saha¹,

Bal²

1993

FEMS Microbiology Letters

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A multiple antibiotic‐resistant Staphylococcus aureus, was found to possess three plasmid bands in agarose gel electrophoresis. A plasmid of approximately 4.3 kb (pMC790/2) was found to code for ampicillin and tetracycline resistance and to have one EcoRI site when transformed into S. aureus RN 4220. pMC790/2 in unmodified form was transformed into a recA−E. coli at a frequency of 1.2×104 transformants/μg of plasmid DNA. Plasmid (pMC790/2) replicated, maintained itself stably and expressed far better in the E. coli host than in S. aureus.

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Section: Introductionmentioning

confidence: 99%