Staphylococcal Spheroplasts and L Coloniest: I. Population Curves of Vegetative Staphylococci and Spheroplasts in Methicillin-Containing Broth over Long Periods

Hamburger, Morton; Carleton, Judith

doi:10.1093/infdis/116.2.221

Cited by 20 publications

(16 citation statements)

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“…Wall-defective forms (L-forms) of strain E1369 were induced by methicillin by a modification of the method of Hamburger and Carleton (20). Strain E1369 was cultivated on hypertonic truche agar containing methicillin for 4 weeks at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Staphylococcus aureus clumping factor

Espersen¹,

Clemmensen²,

Barkholt³

1985

Infect Immun

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Immunochemically identical components were isolated from water-soluble phases of five Staphylococcus aureus strains by affinity chromatography on fibrinogen-linked Sepharose 4B. The elution was performed with 1 M MgCl2. The component could be isolated from sonicated preparations of whole cells, cell walls, and extracellular products of S. aureus but not from sonicated preparations of staphylococcal L-forms or from Staphylococcus epidermidis. Investigations of the eluted component by immunoelectrophoresis and Western blot analysis by use of different polyspecific antibodies to S. aureus raised in rabbits revealed only one immunoprecipitate or one band. By means of gel filtration on Sepharose CL 6B and sodium dodecyl sulfate-polyacrylamide gel electrophoresis a molecular mass of 420,000 and 360,000 was found, respectively. Chemical analysis showed a carbohydrate content of about 20% by weight. By crossed immunoelectrophoresis the isolated component was demonstrated to bind to human fibrinogen. The finding that this purified component inhibited the fibrinogen-induced clumping of staphylococci strongly suggests that the component is the S. aureus clumping factor.

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Section: Methodsmentioning

confidence: 99%

Isolation of Staphylococcus aureus clumping factor

Espersen¹,

Clemmensen²,

Barkholt³

1985

Infect Immun

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“…Antibiotics which inhibit cell wall synthesis and cell wall-lytic enzymes are effective inducing agents (3). Induction with any of these agents requires theinitial formation of cell wall-defective cells which need increased osmotic protection for growth (2,4,5). NaC1 is the most commonly used osmotic stabilizer for staphylococcal L-forms.…”

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confidence: 99%

Salt‐Induced L‐Forms of Staphylococcus aureus

Yabu

1995

Microbiology and Immunology

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“…The morphology, growth characteristics (2,3,14,20), and antimicrobial susceptibility (14,19) of the three stable L-phase variants induced by ~nethicillin (FXSS), cycloserine (CYCS), and lysostaphin (LSTS) were very similar. As part of a comprehensive investigatioil of L-phase variants of S. u~i r e~i s (16), this study was conducted to compare the fatty acid composition of these three stable L-phase variants induced from the same parent S .…”

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confidence: 99%

“…We have successfully induced stable L-phase variants of Stctpliylococc~ts NL/I.EL/S by illethicillin (3,15), cycloserine (14), and lysostaphin (18,20). These three agents have different inodes of action.…”

mentioning

confidence: 99%