Staphylococcal superantigen-like 12 induces the production of interleukin 4 in murine basophils

Nishiyama, Saishi; Urabe, A; Morikawa, Arisa; Kobayashi, Masato; Onozaki, Kikuo; Itoh, Saotomo; Hida, Shigeaki

doi:10.1016/j.bbrc.2020.08.029

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…We previously reported that SSL12 activated two important immune cells in allergic inflammation; SSL12 induced the degranulation and the induction of IL-6 and IL-13 in murine bone marrow-derived mast cells (BMMCs) without cell damage (Kobayashi et al, 2019) and the production of IL-4 in murine bone marrow-derived basophils and murine fresh isolated basophils (Nishiyama et al, 2020). SSL12 would be one of the staphylococcal pathogenic factors in allergic inflammations.…”

Section: Introductionmentioning

confidence: 99%

Identification of responsible amino acid residues in staphylococcal superantigen‐like 12 for the activation of mast cells

et al. 2022

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Staphylococcal superantigen‐like 12 (SSL12) is reported to evoke the degranulation in murine mast cells. The allelic variant of SSL12 in the genome of reference strain NCTC8325 induced the degranulation of murine mast cells, that of MRSA252 strain did not, nevertheless relatively high sequence similarity (82%). To identify responsible amino acid residues of SSL12 for mast cell activation, we created a series of domain swap mutants and amino acid substitution mutants between the active and inactive variants. The mutants that harbored oligonucleotide/oligosaccharide binding (OB)‐fold domain of the active variant activated mast cells. The replacement at position 56 (L56F) in the OB‐fold domain diminished the mast cell stimulatory activity, and the combinatorial substitutions L56F/K92E, L56F/D95S, and L56F/S100V abolished the stimulatory activities of the mutant that harbored OB‐fold domain of the active variant and the intact active variant. These indicate that the responsive elements of SSL12 for mast cell activation are in the OB‐fold of SSL12, and L56 would be an essential amino acid residue for the activation of mast cells. The findings would contribute to the understanding of the molecular mechanism of SSL12 for mast cell activation and the development of toxoids preventing allergic inflammations associated with Staphylococcus aureus.

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Section: Introductionmentioning

confidence: 99%

Identification of responsible amino acid residues in staphylococcal superantigen‐like 12 for the activation of mast cells

et al. 2022

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Staphylococcal γ-hemolysins induce IL-4 production in murine basophils

Ogata

Hayashi

Kitano

et al. 2022

Biochemical and Biophysical Research Communications

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Establishment of a Reporter System to Monitor FcRγ-Dependent Activation of NFAT

Morikawa,

Togawa,

Mukainaka

et al. 2024

BPB Reports

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Basophils are recognized as effectors of type 2 immune responses, producing IL-4 in response to various stimuli such as IL-3 and papain in addition to IgE. In this study, we have established a novel cell-based reporter system that can monitor the activation of the transcription factor NFAT using retroviral vectors. Using this system, we examined whether papain, which is known to induce IL-4 in an FcRγ-dependent manner, could be used to identify its receptor. We created a chimeric receptor in which the extracellular and transmembrane portions are CD8 and the cytoplasmic domain is FcRγ. This chimeric receptor was able to induce GFP in RBL by crosslinking with anti-CD8 antibody. Furthermore, we found that this chimeric receptor can function as a papain receptor, as GFP was upregulated by papain stimulation. On the other hand, statins were able to suppress the expression of GFP by IgE crosslinking. This reporter system can be used to transduce candidate receptors and examine their papain receptor activity using GFP expression as an indicator, and is therefore a useful system that can be used for expression-cloning of unknown papain receptors and for the study of various inhibitors.

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Staphylococcal superantigen-like 12 induces the production of interleukin 4 in murine basophils

Cited by 4 publications

References 27 publications

Identification of responsible amino acid residues in staphylococcal superantigen‐like 12 for the activation of mast cells

Identification of responsible amino acid residues in staphylococcal superantigen‐like 12 for the activation of mast cells

Staphylococcal γ-hemolysins induce IL-4 production in murine basophils

Establishment of a Reporter System to Monitor FcRγ-Dependent Activation of NFAT

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