Staphylococcus aureus clones causing osteomyelitis: a literature review (2000–2020)

Araujo, Fernanda Pimentel de; Monaco, Monica; Grosso, María Del; Pirolo, Mattia; Visca, Paolo; Pantosti, Annalisa

doi:10.1016/j.jgar.2021.03.030

Cited by 39 publications

(25 citation statements)

References 54 publications

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“…Close genetic relatedness between isolates from either the same or different hospitals was rarely observed, suggesting limited transmission of S. aureus strains causing OM within the same hospital and between hospitals in the same geographical area. The S. aureus lineages identified in the present study are largely consistent with those responsible for bone and joint infections worldwide (Pimentel de Araujo et al, 2021), and essentially match the most frequent lineages causing both invasive and non-invasive infections in Italian hospitals (Gagliotti et al, 2012;Grundmann et al, 2014;Giufrè et al, 2017).…”

Section: Discussionsupporting

confidence: 80%

“…The S. aureus lineages identified in the present study are largely consistent with those responsible for bone and joint infections worldwide ( Pimentel de Araujo et al, 2021 ), and essentially match the most frequent lineages causing both invasive and non-invasive infections in Italian hospitals ( Gagliotti et al, 2012 ; Grundmann et al, 2014 ; Giufrè et al, 2017 ). Although ST8/CC8 is the most common clone involved in OM in several countries ( Luedicke et al, 2010 ; Senneville et al, 2014 ; Gaviria-Agudelo et al, 2015 ), the most common lineages in Italy were CC22 and CC5.…”

Section: Discussionsupporting

confidence: 78%

“…S. aureus strains from different sources carry various combinations of virulence genes ( Montanaro et al, 1999 ; Peacock et al, 2002 ; Campoccia et al, 2008 ), and some studies were conducted to identify and characterize the most frequent S. aureus clones cause of OM (reviewed by Pimentel de Araujo et al, 2021 ). Pandemic lineages, including CC5, CC8, CC22, CC30, and CC45, were the most common in OM, and their distribution greatly differed among the countries reflecting the local epidemiology of S. aureus and the MSSA heterogeneity ( Pimentel de Araujo et al, 2021 ). However, the characterization of S. aureus clones causing bone and joint infections in Italy is limited to only few studies ( Campoccia et al, 2008 ; Montanaro et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy

et al. 2022

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Staphylococcus aureus is the most common pathogen causing osteomyelitis (OM). The aim of this study was to explore the clonal complex (CC) distribution and the pattern of virulence determinants of S. aureus isolates from OM in Italy. Whole-genome sequencing was performed on 83 S. aureus isolates from OM cases in six hospitals. Antibiotic susceptibility tests showed that 30.1% of the isolates were methicillin-resistant S. aureus (MRSA). The most frequent CCs detected were CC22, CC5, CC8, CC30, and CC15, which represent the most common lineages circulating in Italian hospitals. MRSA were limited in the number of lineages (CC22, CC5, CC8, and CC1). Phylogenetic analysis followed the sequence type-CC groupings and revealed a non-uniform distribution of the isolates from the different hospitals. No significant difference in the mean number of virulence genes carried by MRSA or MSSA isolates was observed. Some virulence genes, namely cna, fib, fnbA, coa, lukD, lukE, sak, and tst, were correlated with the CC. However, different categories of virulence factors, such as adhesins, exoenzymes, and toxins, were frequently detected and unevenly distributed among all lineages. Indeed, each lineage carried a variable combination of virulence genes, likely reflecting functional redundancy, and arguing for the importance of those traits for the pathogenicity in OM. In conclusion, no specific genetic trait in the most frequent lineages could explain their high prevalence among OM isolates. Our findings highlight that CCs detected in OM isolates follow the epidemiology of S. aureus infections in the country. It is conceivable that any of the most common S. aureus CC can cause a variety of infections, including OM.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

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Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy

et al. 2022

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“…Staphylococcus aureus is associated with both human commensal and clinical infections ( Lowy, 1998 ; Wertheim et al, 2005 ). Staphylococcus aureus can cause skin and soft tissue disease, pleuropulmonary disease, medical device-related bloodstream infections, food poisoning, and even infective endocarditis or osteomyelitis ( Lowy, 1998 ; Lindsay and Holden, 2004 ; Tong et al, 2015 ; Kavanagh et al, 2018 ; Lakhundi and Zhang, 2018 ; Oliveira et al, 2018 ; Turner et al, 2019 ; Horino and Hori, 2020 ; Pimentel de Araujo et al, 2021 ). Although less virulent than S. aureus , CoNS including Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus saprophyticus, Staphylococcus capitis, Staphylococcus lugdunensis, Staphylococcus hominis, Staphylococcus schleiferi , and Staphylococcus warneri are also important staphylococcal pathogen and are usually associated with hospital infections such as skin and soft tissue disease, sepsis, meningitis, endocarditis, and catheter- or implanted device-mediated infections ( Vuong and Otto, 2002 ; Otto, 2009 ; Becker et al, 2014 ; Natsis and Cohen, 2018 ; Azimi et al, 2020 ; Parthasarathy et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Efficiency of Antimicrobial Peptides Against Multidrug-Resistant Staphylococcal Pathogens

Kawada‐Matsuo

Komatsuzawa

2022

Front. Microbiol.

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Antibiotics play a vital role in saving millions of lives from fatal infections; however, the inappropriate use of antibiotics has led to the emergence and propagation of drug resistance worldwide. Multidrug-resistant bacteria represent a significant challenge to treating infections due to the limitation of available antibiotics, necessitating the investigation of alternative treatments for combating these superbugs. Under such circumstances, antimicrobial peptides (AMPs), including human-derived AMPs and bacteria-derived AMPs (so-called bacteriocins), are considered potential therapeutic drugs owing to their high efficacy against infectious bacteria and the poor ability of these microorganisms to develop resistance to them. Several staphylococcal species including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus saprophyticus are commensal bacteria and known to cause many opportunistic infectious diseases. Methicillin-resistant Staphylococci, especially methicillin-resistant S. aureus (MRSA), are of particular concern among the critical multidrug-resistant infectious Gram-positive pathogens. Within the past decade, studies have reported promising AMPs that are effective against MRSA and other methicillin-resistant Staphylococci. This review discusses the sources and mechanisms of AMPs against staphylococcal species, as well as their potential to become chemotherapies for clinical infections caused by multidrug-resistant staphylococci.

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“…[8] S. aureus often infects humans and animals and causes many conditions, such as sepsis, endocarditis, skin infections, pneumonia and osteomyelitis. [9,10,11] Diseases caused by foodborne pathogens have become a major public health problem in developed and developing countries. [12] S. typhimurium and S. aureus are important foodborne pathogens and there is an urgent need to detect them quickly, accurately and sensitively.…”

Section: Introductionmentioning

confidence: 99%

Multiplex polymerase spiral reaction combined with melting curve analysis for simultaneous detection of Salmonella typhimurium and Staphylococcus aureus

Yin

Pang

Huang

et al. 2022

Preprint

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Salmonella typhimurium (S. typhimurium) and Staphylococcus aureus (S. aureus) are common food-borne pathogens that cause food poisoning and acute gastroenteritis in humans. In this study, we developed a method for rapid, sensitive and specific simultaneous determination of S. typhimurium and S. aureus based on multiplex polymerase spiral reaction (m-PSR) and melting curve analysis. Two pairs of primers were designed specifically to target the conserved invA gene sequence of S. typhimurium and nuc gene sequence of S. aureus, and the nucleic acid amplification reaction was achieved using Bst DNA polymerase under isothermal conditions in the same reaction tube. After amplification for 40 min at 61°C, melting curve analysis of the amplification product was carried out. The distinct mean melting temperature allowed simultaneous differentiation of the two target bacteria in the m-PSR assay. The limit of detection of S. typhimurium and S. aureus that could be detected simultaneously was 4.1 × 10−4 ng genomic DNA and 20 CFU/mL pure bacterial culture for each reaction. m-PSR had similar detection limits to multiplex polymerase chain reaction. Based on this method, analysis of contaminated food samples showed excellent sensitivity and specificity consistent with those of pure bacterial cultures. In conclusion, our method is rapid, simultaneous and specific, and promises to be a useful tool for the detection of food-borne pathogens in the food industry.

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Staphylococcus aureus clones causing osteomyelitis: a literature review (2000–2020)

Cited by 39 publications

References 54 publications

Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy

Virulence Determinants in Staphylococcus aureus Clones Causing Osteomyelitis in Italy

Efficiency of Antimicrobial Peptides Against Multidrug-Resistant Staphylococcal Pathogens

Multiplex polymerase spiral reaction combined with melting curve analysis for simultaneous detection of Salmonella typhimurium and Staphylococcus aureus

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