Staphylococcus aureus Disruption of Keratinocytes in Atopic Dermatitis

Steidl, Olivia; Starr, Nicole Lane; Smith, John H.; Kalan, Lindsay; Singh, Anne Marie

doi:10.1016/j.jaci.2021.12.748

Cited by 1 publication

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“…Changes of bacterial species were then linked to skin gene expression profiles, especially dermatitis-related genes (i.e., STAT3 , STAT5B , FLG , CDSN , and ADAM17 ) previously associated with Staphylococcus species, as Staphylococcus aureus -dependent atopic dermatitis have been reported to activate immune system and reduce microbe diversity 35 – 37 (Fig. 4e and Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Spatial multi-omics of human skin reveals KRAS and inflammatory responses to spaceflight

Park,

Overbey,

Narayanan

et al. 2024

Nat Commun

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Spaceflight can change metabolic, immunological, and biological homeostasis and cause skin rashes and irritation, yet the molecular basis remains unclear. To investigate the impact of short-duration spaceflight on the skin, we conducted skin biopsies on the Inspiration4 crew members before (L-44) and after (R + 1) flight. Leveraging multi-omics assays including GeoMx™ Digital Spatial Profiler, single-cell RNA/ATAC-seq, and metagenomics/metatranscriptomics, we assessed spatial gene expressions and associated microbial and immune changes across 95 skin regions in four compartments: outer epidermis, inner epidermis, outer dermis, and vasculature. Post-flight samples showed significant up-regulation of genes related to inflammation and KRAS signaling across all skin regions. These spaceflight-associated changes mapped to specific cellular responses, including altered interferon responses, DNA damage, epithelial barrier disruptions, T-cell migration, and hindered regeneration were located primarily in outer tissue compartments. We also linked epithelial disruption to microbial shifts in skin swab and immune cell activity to PBMC single-cell data from the same crew and timepoints. Our findings present the inaugural collection and examination of astronaut skin, offering insights for future space missions and response countermeasures.

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Section: Resultsmentioning

confidence: 99%