Staphylococcus aureus Mastitis in a White-Eared Opossum (Didelphis albiventris) in the Atlantic Forest of Northeast Brazil

Staphylococcus aureus is a well-known colonizer and cause of infection among animals and it has been described from numerous domestic and wild animal species. The aim of the present study was to investigate the molecular epidemiology of S. aureus in a convenience sample of European wildlife and to review what previously has been observed in the subject field. 124 S. aureus isolates were collected from wildlife in Germany, Austria and Sweden; they were characterized by DNA microarray hybridization and, for isolates with novel hybridization patterns, by multilocus sequence typing (MLST). The isolates were assigned to 29 clonal complexes and singleton sequence types (CC1, CC5, CC6, CC7, CC8, CC9, CC12, CC15, CC22, CC25, CC30, CC49, CC59, CC88, CC97, CC130, CC133, CC398, ST425, CC599, CC692, CC707, ST890, CC1956, ST2425, CC2671, ST2691, CC2767 and ST2963), some of which (ST2425, ST2691, ST2963) were not described previously. Resistance rates in wildlife strains were rather low and mecA-MRSA isolates were rare (n = 6). mecC-MRSA (n = 8) were identified from a fox, a fallow deer, hares and hedgehogs. The common cattle-associated lineages CC479 and CC705 were not detected in wildlife in the present study while, in contrast, a third common cattle lineage, CC97, was found to be common among cervids. No Staphylococcus argenteus or Staphylococcus schweitzeri-like isolates were found. Systematic studies are required to monitor the possible transmission of human- and livestock-associated S. aureus/MRSA to wildlife and vice versa as well as the possible transmission, by unprotected contact to animals. The prevalence of S. aureus/MRSA in wildlife as well as its population structures in different wildlife host species warrants further investigation.

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“…Marsupials: wallabies ( Petrogale lateralis and xanthopus , [56]) and White-Eared Opossum ( Didelphis albiventris , [57]).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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“…To our knowledge, this is the first molecular study and in vitro isolation of S. falcatula -like derived from Northeastern Brazil. Previous molecular analysis of S. falcatula -like isolates in Brazil employed parasites derived from opossums in regions located more than 1,600 km far from Salvador (Cesar et al, 2018; Gondim et al, 2017; Monteiro et al, 2013; Siqueira et al, 2010; Valadas et al, 2016). Finding S. falcatula -like in the Northeast of Brazil, whose genetic and biological characteristics match with isolates detected in at least four different Brazilian states, reinforces the premise that these isolates probably represent a new species shed by opossums, besides S. neurona , S. falcatula , S. speeri and S. lindsayi .…”

Section: Discussionmentioning

confidence: 99%

Sarcocystis falcatula-like derived from opossum in Northeastern Brazil: In vitro propagation in avian cells, molecular characterization and bioassay in birds

Gondim

Soares

Tavares

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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Most reported isolates of Sarcocystis spp. derived from Brazilian opossums ( Didelphis sp.) have genetic characteristics distinct from the known species of Sarcocystis , but behave similarly as Sarcocystis falcatula , as they are infective to budgerigars. In previous studies, these Brazilian isolates, classified as Sarcocystis falcatula -like, were originated from South and Southeast regions of Brazil. In the current work, we aimed to culture and to perform multilocus sequence analysis of Sarcocystis sp. derived from a Brazilian opossum ( D. aurita / D. marsupialis ) that inhabited the city of Salvador, Bahia, in the Northeast of Brazil. The parasite was isolated in Vero cells, referred here as Sarco-BA1, and propagated in avian cells (DF-1). Molecular analysis of Sarco-BA1 revealed that the nucleotide sequence of the internal transcribed spacer 1 (ITS1) of the rDNA was identical to all isolates (n = 19) of Sarcocystis spp. reported in two studies from South and Southeast regions of the country. Two budgerigars were inoculated with 10 and 1000 sporocysts of Sarco-BA1, respectively, and developed acute sarcocystosis, showing that the parasite behaves like S. falcatula . It was interesting to observe that Sarco-BA1 had almost identical ITS1 and SAG sequences to all 16 isolates of S. falcatula -like recently described in Magellanic penguins ( Spheniscus magellanicus ) rescued on the coast of Espírito Santo state, Brazil. Our results suggest that Sarco-BA1 and S. falcatula -like may represent a single species of Sarcocystis . Propagation of the parasite in a permanent avian cell line significantly improved the yield of merozoites in cell culture. To our knowledge, this is the first molecular study and in vitro isolation of S. falcatula -like derived from Northeastern Brazil. Studies are under way to determine the infectivity of Sarco-BA1 to other animal species, as well as to investigate serological cross-reactivity among Sarco-BA1, S. neurona and related species.

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“…(Walther et al., 2008); Colobuses (King colobus ( Colobus polykomos ), Western red colobus ( Piliocolobus badius )) (Heaton et al., 2020); Deer (Roe deer ( Capreolus capreolus ), Silka deer ( Cervus nippon )) (Heaton et al., 2020); Dromedary camel ( Camelus dromedaries ) (Heaton et al., 2020); European badger ( Meles meles ) (Heaton et al., 2020); European beaver ( Castor fiber ) (Heaton et al., 2020); European marmot ( Marmota marmota ) (Heaton et al., 2020); Fox squirrel ( Sciurus niger ) (Heaton et al., 2020); Gabon talapoin ( Miopithecus ogouensis ) (Heaton et al., 2020); Grey‐cheeked mangabey ( Lophocebus albigena ) (Heaton et al., 2020); Harbour porpoise ( Phocoena phocoena ) (Heaton et al., 2020); Killer whale ( Orcinus orca ) (Power and Murphy, 2002); Lion ( Panthera leo ) (Heaton et al., 2020); Macaques (Japanese macaque ( Macaca fuscata ), Barbary macaque ( Macaca sylvanus )) (Heaton et al., 2020); Malayan tapir ( Tapirus indicus ) (Heaton et al., 2020); Mandrill ( Mandrillus sp.) (Heaton et al., 2020); Mice (Yellow‐necked mouse ( Apodemus flavicollis ), House mouse ( Mus musculus ) (Heaton et al., 2020); Mongolian sheep ( Ovis ammon f. aries ) (Heaton et al., 2020); Monkeys (Greater spot‐nose monkey ( Cercopithecus nictitans ), Red‐tailed monkey ( Cercopithecus ascanius )) (Heaton et al., 2020); Moose ( Alces alces ) (Heaton et al., 2020); Mouflons (European mouflon, Mouflon ( Ovis orientalis )) (Heaton et al., 2020); Moustached guenon ( Cercopithecus cephus ) (Heaton et al., 2020); Northern white‐breasted hedgehog ( Erinaceus roumanicus ) (Heaton et al., 2020); Pygmy goat ( Capra hircus ) (Heaton et al., 2020); Raccoon ( Procyon lotor ) (Plommet and Wilson, 1969); Reindeer ( Rangifer tarandus ) (Heaton et al., 2020); Voles (Bank vole ( Myodes glareolus ), Field vole ( Microtus agrestis )) (Heaton et al., 2020); White‐eared opossum ( Didelphis albiventris ) (Siqueira et al., 2010); Wild cats (European wildcat ( Felis silvestris ), African wildcat ( Felis lybica )) (Heaton et al., 2020); Yellow‐footed rock wallaby ( Petrogale xanthopus ) (Heaton et al., 2020). …”

Section: Assessmentmentioning

confidence: 99%

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): antimicrobial‐resistant Staphylococcus aureus in cattle and horses

Nielsen¹,

Bicout²,

Calistri³

et al. 2022

EFS2

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Staphylococcus aureus ( S. aureus ) was identified among the most relevant antimicrobial‐resistant (AMR) bacteria in the EU for cattle and horses in previous scientific opinions. Thus, it has been assessed according to the criteria of the Animal Health Law (AHL), in particular criteria of Article 7 on disease profile and impacts, Article 5 on its eligibility to be listed, Annex IV for its categorisation according to disease prevention and control rules as in Article 9, and Article 8 for listing animal species related to the bacterium. The assessment has been performed following a methodology previously published. The outcome is the median of the probability ranges provided by the experts, which indicates whether each criterion is fulfilled (lower bound ≥ 66%) or not (upper bound ≤ 33%), or whether there is uncertainty about fulfilment. Reasoning points are reported for criteria with uncertain outcome. According to the assessment here performed, it is uncertain whether AMR S. aureus can be considered eligible to be listed for Union intervention according to Article 5 of the AHL (60–90% probability). According to the criteria in Annex IV, for the purpose of categorisation related to the level of prevention and control as in Article 9 of the AHL, the AHAW Panel concluded that the bacterium does not meet the criteria in Sections 1, 2 and 4 (Categories A, B and D; 1–5%, 5–10% and 10–33% probability of meeting the criteria, respectively) and the AHAW Panel was uncertain whether it meets the criteria in Sections 3 and 5 (Categories C and E, 33–90% and 60–90% probability of meeting the criteria, respectively). The animal species to be listed for AMR S. aureus according to Article 8 criteria include mainly mammals, birds, reptiles and fish.

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Staphylococcus aureus Mastitis in a White-Eared Opossum (Didelphis albiventris) in the Atlantic Forest of Northeast Brazil

Cited by 9 publications

References 5 publications

Diversity of Staphylococcus aureus Isolates in European Wildlife

Diversity of Staphylococcus aureus Isolates in European Wildlife

Sarcocystis falcatula-like derived from opossum in Northeastern Brazil: In vitro propagation in avian cells, molecular characterization and bioassay in birds

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): antimicrobial‐resistant Staphylococcus aureus in cattle and horses

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