2011
DOI: 10.1186/1297-9716-42-35
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Staphylococcus aureus seroproteomes discriminate ruminant isolates causing mild or severe mastitis

Abstract: Staphylococcus aureus is a major cause of mastitis in ruminants. In ewe mastitis, symptoms range from subclinical to gangrenous mastitis. S. aureus factors or host-factors contributing to the different outcomes are not completely elucidated. In this study, experimental mastitis was induced on primiparous ewes using two S. aureus strains, isolated from gangrenous (strain O11) or subclinical (strain O46) mastitis. Strains induced drastically distinct clinical symptoms when tested in ewe and mice experimental mas… Show more

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Cited by 38 publications
(56 citation statements)
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“…We characterized two S. aureus ovine strains, which were shown to be clonally related (9) and reproducibly induced severe (strain O11) and mild (strain O46) mastitis in experimental ewe mastitis (8).…”
mentioning
confidence: 99%
“…We characterized two S. aureus ovine strains, which were shown to be clonally related (9) and reproducibly induced severe (strain O11) and mild (strain O46) mastitis in experimental ewe mastitis (8).…”
mentioning
confidence: 99%
“…Similarly, Cphp4 and Cphp7 presented conserved HtaA domains found in secreted proteins involved in iron uptake and transport (Drazek et al, 2000). As shown for S. aureus in mastitis (Le Maré chal et al, 2011), it seems that C. pseudotuberculosis expresses proteins related to iron acquisition and metabolism in the CLA context.…”
Section: Resultsmentioning
confidence: 84%
“…Pellets were solubilized in sample solution containing 7 M urea, 2 M thio-urea, 25 mM dithiothreitol (DTT), 4% (w/v) 3-[(3-cholamidopropyl)dimethylammonio]-1-propane-sulfonate (CHAPS), and 2% (w/v) ampholyte containing buffer (IPG-Buffer 3-10 NL, GE Healthcare, Orsay, France). Isoelectric focusing was carried out using 18 cm Immobiline Dry Strips on a Multiphor II electrophoresis system (Amersham Biosciences) using a standard procedure described previously (Le Maré chal et al, 2011). The second dimensional separation was performed using a 12% polyacrylamide gel without stacking on an Ettan TM DALTtwelve electrophoresis system (GE Healthcare) in TrisGlycine-SDS buffer at 180 V for 9 h. The gels were stained with colloidal Coomassie blue and then cleared with an aqueous solution containing 10% acetic acid and 30% methanol.…”
Section: Serpa Of C Pseudotuberculosismentioning
confidence: 99%
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