Staphylococcus aureus surface protein G (sasG) allelic variants: correlation between biofilm formation and their prevalence in methicillin-resistant S. aureus (MRSA) clones

Carrera-Salinas, Anna; González-Díaz, Aida; Vázquez-Sánchez, Daniel Antonio; Camoez, Mariana; Niubó, Jordi; Càmara, Jordi; Ardanuy, Carmen; Martí, Sara; Domínguez, M. Ángeles

doi:10.1016/j.resmic.2022.103921

Cited by 9 publications

(10 citation statements)

References 31 publications

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“…An interesting observation was the length of this gene. It was longer than sasG from published sequences of isolates mainly derived from humans [ 43 ], with an average length among the sasG -positive study strains of about 5442 nt (median, 5268 nt; range, 4116 to 7188 nt). This difference was related to the number of the repeat units this gene comprises, translating into a presence of 9 full repeats (median; ranging from 6 to 14 repeats; Table 2 ; Figure 2 ) plus 1 additional, truncated terminal repeat (referred to as “½ repeat” in Table 2 , see also below).…”

Section: Resultsmentioning

confidence: 99%

“…This difference was related to the number of the repeat units this gene comprises, translating into a presence of 9 full repeats (median; ranging from 6 to 14 repeats; Table 2 ; Figure 2 ) plus 1 additional, truncated terminal repeat (referred to as “½ repeat” in Table 2 , see also below). A recent work analysed 353 sasG sequences identified in GenBank from full genomes mostly of human strains [ 43 ]. These contained a median of only 3 full repeats (range from 0 to 9 repeats), plus the 1 truncated terminal repeat.…”

Section: Resultsmentioning

confidence: 99%

“…One can be found in CC1 and a number of sporadic lineages. The other one is present in CC5 and CC8 [ 43 ]. They can be discerned based not on the numbers, which are variable, but on the actual sequence of the repeat units and on the sequence of the “A domain” of the deduced protein [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

“…The other one is present in CC5 and CC8 [ 43 ]. They can be discerned based not on the numbers, which are variable, but on the actual sequence of the repeat units and on the sequence of the “A domain” of the deduced protein [ 43 ]. In the CC1-like allele, lengths (128 amino acids, aa) and sequences of all repeats are uniform, except for the last one, adjacent to the anchor, which is a shorter, truncated version of the others (75 aa rather than 128 aa; Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…The sasG gene was longer, containing a higher number of repeating units than sasG from human isolates usually does [ 43 ], regardless of the allelic variant actually present. Whether this was a host specific adaption needs still to be determined.…”

Section: Discussionmentioning

confidence: 99%

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Sequence Analysis of Novel Staphylococcus aureus Lineages from Wild and Captive Macaques

Monecke

Roberts

Diezel

et al. 2022

IJMS

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Staphylococcus aureus is a widespread and common opportunistic bacterium that can colonise or infect humans as well as a wide range of animals. There are a few studies of both methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolated from monkeys, apes, and lemurs, indicating a presence of a number of poorly or unknown lineages of the pathogen. In order to obtain insight into staphylococcal diversity, we sequenced strains from wild and captive individuals of three macaque species (Macaca mulatta, M. assamensis, and M. sylvanus) using Nanopore and Illumina technologies. These strains were previously identified by microarray as poorly or unknown strains. Isolates of novel lineages ST4168, ST7687, ST7688, ST7689, ST7690, ST7691, ST7692, ST7693, ST7694, ST7695, ST7745, ST7746, ST7747, ST7748, ST7749, ST7750, ST7751, ST7752, ST7753, and ST7754 were sequenced and characterised for the first time. In addition, isolates belonging to ST2990, a lineage also observed in humans, and ST3268, a MRSA strain already known from macaques, were also included into the study. Mobile genetic elements, genomic islands, and carriage of prophages were analysed. There was no evidence for novel host-specific virulence factors. However, a conspicuously high rate of carriage of a pathogenicity island harbouring edinB and etD2/etE as well as a higher number of repeat units within the gene sasG (encoding an adhesion factor) than in human isolates were observed. None of the strains harboured the genes encoding Panton–Valentine leukocidin. In conclusion, wildlife including macaques may harbour an unappreciated diversity of S. aureus lineages that may be of clinical relevance for humans, livestock, or for wildlife conservation, given the declining state of many wildlife populations.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%