Staphylococcus delphini sp. nov., a Coagulase-Positive Species Isolated from Dolphins

Varaldo, Pietro E.; Kilpper-Bälz, R.; Biavasco, Francesca; Satta, G.; Schleifer, Karl Heinz

doi:10.1099/00207713-38-4-436

Cited by 107 publications

(58 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Beck and Rice (2003) reported on antibody levels in sera against several pathogenic and nonpathogenic bacteria in Atlantic ocean bottlenose dolphins. Potential human pathogens, previously unknown in marine mammals, also have been reported from numerous cetacean species and emphasize the need for baseline data (Varaldo et al, 1988;Neese et al, 1993;Buck and McCarthy, 1994;Foster et al, 1996;Swenshon et al, 1998;Dunn et al, 2001;Harper et al, 2002Harper et al, , 2003Miller et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Aerobic Microorganisms Associated With Free-Ranging Bottlenose Dolphins in Coastal Gulf of Mexico and Atlantic Ocean Waters

Buck

Wells

Rhinehart

et al. 2006

Journal of Wildlife Diseases

View full text Add to dashboard Cite

ABSTRACT:Our abilities to assess health risks to free-ranging dolphin populations, to treat livestranded or captive dolphins, and to evaluate the risks of disease transmission between humans and dolphins have suffered from a lack of basic information on microorganisms associated with normal, presumably healthy free-ranging individuals. In order to provide these data, we sampled freeranging bottlenose dolphins (Tursiops truncatus) off Florida, Texas, and North Carolina during 1990-2002. Blowhole and anal/fecal samples yielded 1,871 bacteria and yeast isolates and included 85 different species or groups of organisms. Vibrios, unidentified pseudomonads, Escherichia coli, Staphylococcus spp., and a large group of nonfermenting Gram-negative bacteria represented .50% of isolates. Vibrio alginolyticus and Vibrio damsela were the most commonly recovered bacteria from both anal/fecal and blowhole samples. Many organisms occurred sporadically in dolphins that were sampled repeatedly, but some were consistently isolated from individual animals and may indicate the carrier state. Vibrios were common, but some geographic variability in the presence of these and other organisms was noted. Potential pathogens of significance to humans and other animals were recovered.

show abstract

Section: Introductionmentioning

confidence: 99%

Aerobic Microorganisms Associated With Free-Ranging Bottlenose Dolphins in Coastal Gulf of Mexico and Atlantic Ocean Waters

Buck

Wells

Rhinehart

et al. 2006

Journal of Wildlife Diseases

View full text Add to dashboard Cite

show abstract

“…The type strain of this species is strain MB4 (= CCM 4175). (5,11,21,26). Most of the new species were isolated from animals; the exceptions were S .…”

mentioning

confidence: 99%

“…A total of 28 species are currently recognized in the genus Staphylococcus; 19 species are listed in Bergey's Manual of Systematic Bacteriology (14), two species (Staphylococcus chromogenes and Staphylococcus lentus) have been elevated from the subspecies level (8, 19), and 7 species have been described since 1984 (Staphylococcus arlettae, Staphylococcus delphini, Staphylococcus equorum, Staphylococcus felis, Staphylococcus kloosii, Staphylococcus lugdunensis, and Staphylococcus schleiferi) (5,11,21,26). Most of the new species were isolated from animals; the exceptions were S .…”

mentioning

confidence: 99%

Staphylococcus muscae, a New Species Isolated from Flies

Hájek

Ludwig

Schleifer

et al. 1992

International Journal of Systematic Bacteriology

Self Cite

View full text Add to dashboard Cite

A new coagulase-negative species of the genus Staphylococcus, Staphylococcus muscae, is described on the basis of the results of a study of four strains that were isolated from flies. 16s rRNA sequences of the type strains of S . muscae, Staphylococcus schleiferi, and staphylococcus sciuri were determined and used, together with the corresponding sequences of Staphylococcus aureus and Staphylococcus epidermidis, for a comparative analysis. The new species is characterized taxonomically; this species is differentiated from the other novobiocin-susceptible staphylococci by its physiological and biochemical activities, cell wall composition, and levels of genetic relatedness. The type strain of this species is strain MB4 (= CCM 4175). (5,11,21,26). Most of the new species were isolated from animals; the exceptions were S . lugdunensis and S. schleiferi, which were isolated from humans. The descriptions of all 28 Staphylococcus species were based on studies of strains that were isolated from mammals, birds, and foodstuffs of animal origin.In this paper, we describe a new coagulase-negative species of staphylococci that was obtained from the body surfaces of flies. These organisms were found in about 7% of the flies caught in certain cow sheds but not on flies caught in human dwellings, stables, or piggeries (7). For this reason, this bacterium should be regarded as a transient rather than a resident on flies.In this report we present phenetic and molecular taxonomic data for a new species, Staphylococcus muscae. MATERIALS AND METHODSBacterial strains. Four strains were isolated from flies trapped in cow sheds; strains MB4T, (T = type strain), MB21, and MB50 were isolated from Stomoxys calcitrans, and strain MB30 was isolated from Musca domestica (7). The medium used for isolation was blood agar base no. 2 (Oxoid) supplemented with 5% defibrinated ovine blood. Nutrient agar (Oxoid) containing glucose (2%, wt/vol) and P agar (15) were used to propagate all of the isolates. Inocula for tests were prepared from 1-day cultures that were incubated at 37°C. All strains were stored as frozen suspensions in glycerol broth at -25°C (10) and also in a freezedried state.Methods. The procedures used for determining morphological, physiological, and biochemical characteristics have been described elsewhere (6). The type strains of the 14 novobiocin-susceptible Staphylococcus species (see Table 2) * Corresponding author. 97were obtained from the Deutsche Sammlung von Mikroorganismen (DSM), Braunschweig, Germany, the American Type Culture Collection (ATCC), Rockville, Md., and the Czechoslovak Collection of Microorganisms (CCM), Brno, Czechoslovakia.Phage typing was carried out by using the method of Blair and Williams (1). We used 24 phages (25) for human Staphylococcus aureus strains, 12 phages (24) for bovine S. aureus strains, 4 experimental phages (2) for Staphylococcus intermedius strains, and 4 phages (9) for Staphylococcus hyicus strains.We used the methods described by Schleifer and Kandler (20) and Schleifer (18) for cel...

show abstract

“…Skin lesions containing Staphylococcus were reported for the first time in cetaceans in 1988 [27]. In that study, two isolates of Staphylococcus were obtained from purulent tissue that occurred in two captive dolphins with multiple, suppurating lesions, and subsequently described as a new species, Staphylococcus delphini.…”

Section: Case Studies Of Skin Lesions In Cetaceans -The Signs?mentioning

confidence: 99%

“…These include species of Brucella, Corynebacterium, Dermatophilus, Escherichia, Erysipelothrix, Klebsiella, Mycobacterium, Pseudomonas, Staphylococcus, Streptococcus and Vibrio (Table 1) [27,47,55]. Bacteria also cause death of cetaceans in many cases [55].…”

Section: Bacterial Infections Of Cetacean Skinmentioning

confidence: 99%